Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glyQ and Shew_0011 are separated by 109 nucleotides Shew_0011 and Shew_0012 are separated by 5 nucleotides Shew_0012 and Shew_0013 are separated by 212 nucleotides
Shew_0010: glyQ - glycyl-tRNA synthetase subunit alpha (RefSeq), at 11,589 to 12,494
glyQ
Shew_0011: Shew_0011 - DNA-3-methyladenine glycosylase I (RefSeq), at 12,604 to 13,173
_0011
Shew_0012: Shew_0012 - hypothetical protein (RefSeq), at 13,179 to 13,859
_0012
Shew_0013: Shew_0013 - amidohydrolase (RefSeq), at 14,072 to 17,275
_0013
Position (kb) 12
13
14 Strain fitness (log2 ratio) -1
0
1
2 at 12.666 kb on + strand, within Shew_0011 at 12.690 kb on - strand, within Shew_0011 at 12.744 kb on - strand, within Shew_0011 at 12.828 kb on + strand, within Shew_0011 at 12.836 kb on - strand, within Shew_0011 at 12.875 kb on + strand, within Shew_0011 at 12.883 kb on - strand, within Shew_0011 at 12.986 kb on - strand, within Shew_0011 at 13.201 kb on + strand at 13.245 kb on - strand at 13.247 kb on + strand, within Shew_0012 at 13.584 kb on - strand, within Shew_0012 at 13.675 kb on + strand, within Shew_0012 at 13.726 kb on + strand, within Shew_0012 at 13.726 kb on + strand, within Shew_0012 at 13.726 kb on + strand, within Shew_0012 at 13.726 kb on + strand, within Shew_0012 at 13.734 kb on - strand, within Shew_0012 at 13.734 kb on - strand, within Shew_0012 at 13.734 kb on - strand, within Shew_0012 at 13.786 kb on - strand, within Shew_0012 at 13.876 kb on + strand at 13.882 kb on - strand at 14.171 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 12,666 + Shew_0011 0.11 +0.1 12,690 - Shew_0011 0.15 +1.9 12,744 - Shew_0011 0.25 -0.9 12,828 + Shew_0011 0.39 -0.1 12,836 - Shew_0011 0.41 -0.6 12,875 + Shew_0011 0.48 -0.0 12,883 - Shew_0011 0.49 +0.2 12,986 - Shew_0011 0.67 +1.1 13,201 + -0.5 13,245 - -0.6 13,247 + Shew_0012 0.10 +1.1 13,584 - Shew_0012 0.59 +0.6 13,675 + Shew_0012 0.73 -1.2 13,726 + Shew_0012 0.80 -0.2 13,726 + Shew_0012 0.80 +1.0 13,726 + Shew_0012 0.80 +0.0 13,726 + Shew_0012 0.80 -1.3 13,734 - Shew_0012 0.81 -1.6 13,734 - Shew_0012 0.81 +0.2 13,734 - Shew_0012 0.81 +0.3 13,786 - Shew_0012 0.89 +0.2 13,876 + -0.0 13,882 - -0.1 14,171 - +0.3
Or see this region's nucleotide sequence