Experiment: Flask grown at 18C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_2433 and ilvH are separated by 16 nucleotides ilvH and Synpcc7942_2435 are separated by 3 nucleotides Synpcc7942_2435 and msrA are separated by 43 nucleotides msrA and ileS are separated by 58 nucleotides
Synpcc7942_2433: Synpcc7942_2433 - hypothetical protein, at 2,506,726 to 2,507,646
_2433
Synpcc7942_2434: ilvH - acetolactate synthase 3 regulatory subunit, at 2,507,663 to 2,508,181
ilvH
Synpcc7942_2435: Synpcc7942_2435 - hypothetical protein, at 2,508,185 to 2,508,805
_2435
Synpcc7942_2436: msrA - peptide methionine sulfoxide reductase, at 2,508,849 to 2,509,445
msrA
Synpcc7942_2437: ileS - isoleucyl-tRNA synthetase, at 2,509,504 to 2,512,368
ileS
Position (kb)
2508
2509 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2507.619 kb on - strand at 2507.619 kb on - strand at 2508.175 kb on + strand at 2508.175 kb on + strand at 2508.175 kb on + strand at 2508.175 kb on + strand at 2508.175 kb on + strand at 2508.175 kb on + strand at 2508.175 kb on + strand at 2508.175 kb on + strand at 2508.183 kb on - strand at 2508.183 kb on - strand at 2508.183 kb on - strand at 2508.183 kb on - strand at 2508.183 kb on - strand at 2508.183 kb on - strand at 2508.183 kb on - strand at 2508.183 kb on - strand at 2508.183 kb on - strand at 2508.183 kb on - strand at 2508.183 kb on - strand at 2508.183 kb on - strand at 2508.204 kb on - strand at 2508.212 kb on + strand at 2508.242 kb on + strand at 2508.242 kb on + strand at 2508.244 kb on + strand at 2508.275 kb on + strand, within Synpcc7942_2435 at 2508.275 kb on + strand, within Synpcc7942_2435 at 2508.275 kb on + strand, within Synpcc7942_2435 at 2508.275 kb on + strand, within Synpcc7942_2435 at 2508.283 kb on - strand, within Synpcc7942_2435 at 2508.283 kb on - strand, within Synpcc7942_2435 at 2508.283 kb on - strand, within Synpcc7942_2435 at 2508.283 kb on - strand, within Synpcc7942_2435 at 2508.283 kb on - strand, within Synpcc7942_2435 at 2508.405 kb on + strand, within Synpcc7942_2435 at 2508.616 kb on - strand, within Synpcc7942_2435 at 2508.631 kb on - strand at 2508.681 kb on - strand, within Synpcc7942_2435 at 2508.690 kb on - strand, within Synpcc7942_2435 at 2508.833 kb on + strand at 2508.843 kb on - strand at 2508.845 kb on + strand at 2508.848 kb on - strand at 2508.855 kb on - strand at 2508.868 kb on - strand at 2508.871 kb on + strand at 2508.871 kb on + strand at 2508.879 kb on - strand at 2508.963 kb on + strand, within msrA at 2508.963 kb on + strand, within msrA at 2508.971 kb on - strand, within msrA at 2509.031 kb on + strand, within msrA at 2509.458 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown at 18C remove 2,507,619 - +1.3 2,507,619 - +1.4 2,508,175 + +1.4 2,508,175 + +0.5 2,508,175 + +0.2 2,508,175 + -2.6 2,508,175 + -1.1 2,508,175 + +0.2 2,508,175 + -2.2 2,508,175 + -2.4 2,508,183 - +1.0 2,508,183 - -0.6 2,508,183 - +0.8 2,508,183 - +0.3 2,508,183 - +1.0 2,508,183 - -0.6 2,508,183 - +1.0 2,508,183 - +2.8 2,508,183 - -1.6 2,508,183 - +0.5 2,508,183 - +1.1 2,508,183 - -0.4 2,508,204 - +1.1 2,508,212 + -0.9 2,508,242 + +2.5 2,508,242 + -2.0 2,508,244 + +0.9 2,508,275 + Synpcc7942_2435 0.14 +0.5 2,508,275 + Synpcc7942_2435 0.14 +1.4 2,508,275 + Synpcc7942_2435 0.14 -1.1 2,508,275 + Synpcc7942_2435 0.14 +1.6 2,508,283 - Synpcc7942_2435 0.16 -1.7 2,508,283 - Synpcc7942_2435 0.16 +1.0 2,508,283 - Synpcc7942_2435 0.16 +1.9 2,508,283 - Synpcc7942_2435 0.16 -0.9 2,508,283 - Synpcc7942_2435 0.16 +3.3 2,508,405 + Synpcc7942_2435 0.35 +1.5 2,508,616 - Synpcc7942_2435 0.69 +0.5 2,508,631 - -1.1 2,508,681 - Synpcc7942_2435 0.80 -2.9 2,508,690 - Synpcc7942_2435 0.81 +2.1 2,508,833 + +0.1 2,508,843 - +0.8 2,508,845 + -0.1 2,508,848 - +1.4 2,508,855 - +2.8 2,508,868 - -0.0 2,508,871 + -3.4 2,508,871 + -1.1 2,508,879 - +1.9 2,508,963 + msrA Synpcc7942_2436 0.19 +0.1 2,508,963 + msrA Synpcc7942_2436 0.19 +0.6 2,508,971 - msrA Synpcc7942_2436 0.20 +0.2 2,509,031 + msrA Synpcc7942_2436 0.30 +0.3 2,509,458 + -1.9
Or see this region's nucleotide sequence