Experiment: Flask grown at 18C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_1445 and Synpcc7942_1446 are separated by 105 nucleotides Synpcc7942_1446 and icfA are separated by 146 nucleotides icfA and nadA are separated by 35 nucleotides
Synpcc7942_1445: Synpcc7942_1445 - hypothetical protein, at 1,498,702 to 1,499,817
_1445
Synpcc7942_1446: Synpcc7942_1446 - hypothetical protein, at 1,499,923 to 1,500,342
_1446
Synpcc7942_1447: icfA - Carbonate dehydratase, at 1,500,489 to 1,501,307
icfA
Synpcc7942_1448: nadA - quinolinate synthetase, at 1,501,343 to 1,502,305
nadA
Position (kb)
1500
1501
1502 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 1499.489 kb on + strand, within Synpcc7942_1445 at 1499.489 kb on - strand, within Synpcc7942_1445 at 1499.491 kb on - strand, within Synpcc7942_1445 at 1499.496 kb on + strand, within Synpcc7942_1445 at 1499.517 kb on + strand, within Synpcc7942_1445 at 1499.525 kb on - strand, within Synpcc7942_1445 at 1499.539 kb on + strand, within Synpcc7942_1445 at 1499.577 kb on - strand, within Synpcc7942_1445 at 1499.589 kb on + strand, within Synpcc7942_1445 at 1499.644 kb on - strand, within Synpcc7942_1445 at 1499.779 kb on - strand at 1499.792 kb on + strand at 1499.802 kb on - strand at 1499.837 kb on - strand at 1499.903 kb on - strand at 1499.905 kb on + strand at 1499.910 kb on - strand at 1499.987 kb on - strand, within Synpcc7942_1446 at 1500.016 kb on - strand, within Synpcc7942_1446 at 1500.107 kb on + strand, within Synpcc7942_1446 at 1500.115 kb on - strand, within Synpcc7942_1446 at 1500.123 kb on + strand, within Synpcc7942_1446 at 1500.232 kb on + strand, within Synpcc7942_1446 at 1500.232 kb on + strand, within Synpcc7942_1446 at 1500.235 kb on - strand, within Synpcc7942_1446 at 1500.245 kb on - strand, within Synpcc7942_1446 at 1500.254 kb on + strand, within Synpcc7942_1446 at 1500.261 kb on + strand, within Synpcc7942_1446 at 1500.269 kb on - strand, within Synpcc7942_1446 at 1500.279 kb on - strand, within Synpcc7942_1446 at 1500.313 kb on + strand at 1500.316 kb on - strand at 1500.318 kb on + strand at 1500.321 kb on - strand at 1500.321 kb on - strand at 1500.326 kb on - strand at 1500.326 kb on - strand at 1500.351 kb on + strand at 1500.351 kb on + strand at 1500.359 kb on - strand at 1500.359 kb on - strand at 1500.359 kb on - strand at 1500.359 kb on - strand at 1500.363 kb on + strand at 1500.369 kb on + strand at 1500.370 kb on - strand at 1500.392 kb on - strand at 1500.402 kb on - strand at 1500.456 kb on + strand at 1500.469 kb on - strand at 1500.471 kb on + strand at 1501.317 kb on + strand at 1501.326 kb on - strand at 1501.337 kb on - strand at 1501.653 kb on - strand, within nadA at 1501.726 kb on + strand, within nadA at 1501.726 kb on + strand, within nadA at 1501.903 kb on - strand, within nadA at 1501.974 kb on - strand, within nadA at 1502.026 kb on + strand, within nadA at 1502.074 kb on - strand, within nadA at 1502.130 kb on - strand, within nadA at 1502.138 kb on + strand, within nadA at 1502.157 kb on + strand, within nadA at 1502.290 kb on + strand at 1502.305 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown at 18C remove 1,499,489 + Synpcc7942_1445 0.71 +0.9 1,499,489 - Synpcc7942_1445 0.71 +0.3 1,499,491 - Synpcc7942_1445 0.71 -0.1 1,499,496 + Synpcc7942_1445 0.71 -0.4 1,499,517 + Synpcc7942_1445 0.73 +1.6 1,499,525 - Synpcc7942_1445 0.74 -0.1 1,499,539 + Synpcc7942_1445 0.75 -2.1 1,499,577 - Synpcc7942_1445 0.78 -2.5 1,499,589 + Synpcc7942_1445 0.79 +0.8 1,499,644 - Synpcc7942_1445 0.84 -0.2 1,499,779 - +0.5 1,499,792 + -1.4 1,499,802 - -2.8 1,499,837 - -2.1 1,499,903 - +0.7 1,499,905 + -0.7 1,499,910 - +1.1 1,499,987 - Synpcc7942_1446 0.15 -3.0 1,500,016 - Synpcc7942_1446 0.22 +0.5 1,500,107 + Synpcc7942_1446 0.44 +3.1 1,500,115 - Synpcc7942_1446 0.46 -0.0 1,500,123 + Synpcc7942_1446 0.48 -2.1 1,500,232 + Synpcc7942_1446 0.74 +0.2 1,500,232 + Synpcc7942_1446 0.74 -1.5 1,500,235 - Synpcc7942_1446 0.74 -3.3 1,500,245 - Synpcc7942_1446 0.77 -2.2 1,500,254 + Synpcc7942_1446 0.79 +0.9 1,500,261 + Synpcc7942_1446 0.80 -0.9 1,500,269 - Synpcc7942_1446 0.82 -1.4 1,500,279 - Synpcc7942_1446 0.85 -0.4 1,500,313 + -1.6 1,500,316 - -0.6 1,500,318 + +0.4 1,500,321 - +0.9 1,500,321 - -1.0 1,500,326 - +1.2 1,500,326 - +0.5 1,500,351 + +0.3 1,500,351 + +0.7 1,500,359 - +1.2 1,500,359 - +0.1 1,500,359 - -1.0 1,500,359 - +0.1 1,500,363 + -0.6 1,500,369 + -0.7 1,500,370 - +0.0 1,500,392 - -5.0 1,500,402 - +0.5 1,500,456 + +0.4 1,500,469 - -2.0 1,500,471 + -0.9 1,501,317 + -2.5 1,501,326 - -1.2 1,501,337 - -4.9 1,501,653 - nadA Synpcc7942_1448 0.32 -2.4 1,501,726 + nadA Synpcc7942_1448 0.40 -3.4 1,501,726 + nadA Synpcc7942_1448 0.40 -3.3 1,501,903 - nadA Synpcc7942_1448 0.58 -2.0 1,501,974 - nadA Synpcc7942_1448 0.66 -1.2 1,502,026 + nadA Synpcc7942_1448 0.71 -2.1 1,502,074 - nadA Synpcc7942_1448 0.76 -1.0 1,502,130 - nadA Synpcc7942_1448 0.82 -2.0 1,502,138 + nadA Synpcc7942_1448 0.83 -1.4 1,502,157 + nadA Synpcc7942_1448 0.85 -1.8 1,502,290 + -2.8 1,502,305 + +0.9
Or see this region's nucleotide sequence