Experiment: Flask grown at 18C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mntC and mntA are separated by 58 nucleotides mntA and mntB are separated by 6 nucleotides mntB and Synpcc7942_1319 are separated by 65 nucleotides Synpcc7942_1319 and Synpcc7942_1320 overlap by 34 nucleotides
Synpcc7942_1316: mntC - Mn transporter MntC, at 1,346,077 to 1,347,114
mntC
Synpcc7942_1317: mntA - ATPase, at 1,347,173 to 1,347,910
mntA
Synpcc7942_1318: mntB - manganese transport system membrane protein MntB, at 1,347,917 to 1,348,768
mntB
Synpcc7942_1319: Synpcc7942_1319 - pyrimidine regulatory protein PyrR, at 1,348,834 to 1,349,373
_1319
Synpcc7942_1320: Synpcc7942_1320 - hypothetical protein, at 1,349,340 to 1,350,296
_1320
Position (kb) 1347
1348
1349 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 1346.943 kb on - strand, within mntC at 1346.948 kb on - strand, within mntC at 1346.975 kb on + strand, within mntC at 1346.982 kb on + strand, within mntC at 1346.988 kb on - strand, within mntC at 1346.990 kb on + strand, within mntC at 1346.990 kb on + strand at 1346.998 kb on - strand, within mntC at 1347.002 kb on + strand, within mntC at 1347.002 kb on + strand, within mntC at 1347.002 kb on + strand, within mntC at 1347.002 kb on + strand, within mntC at 1347.010 kb on - strand, within mntC at 1347.010 kb on - strand, within mntC at 1347.010 kb on - strand, within mntC at 1347.010 kb on - strand at 1347.010 kb on - strand, within mntC at 1347.010 kb on - strand, within mntC at 1347.020 kb on - strand at 1347.020 kb on - strand at 1347.038 kb on + strand at 1347.044 kb on + strand at 1347.044 kb on - strand at 1347.062 kb on + strand at 1347.075 kb on - strand at 1347.075 kb on - strand at 1347.075 kb on - strand at 1347.080 kb on + strand at 1347.091 kb on + strand at 1347.103 kb on - strand at 1347.117 kb on + strand at 1347.125 kb on - strand at 1347.125 kb on - strand at 1347.125 kb on - strand at 1347.127 kb on + strand at 1347.190 kb on - strand at 1347.250 kb on - strand, within mntA at 1347.263 kb on + strand, within mntA at 1347.263 kb on + strand, within mntA at 1347.277 kb on + strand, within mntA at 1347.311 kb on + strand, within mntA at 1347.311 kb on + strand, within mntA at 1347.319 kb on - strand, within mntA at 1347.319 kb on - strand, within mntA at 1347.322 kb on + strand, within mntA at 1347.344 kb on + strand, within mntA at 1347.354 kb on - strand, within mntA at 1347.379 kb on + strand, within mntA at 1347.379 kb on + strand, within mntA at 1347.398 kb on - strand, within mntA at 1347.424 kb on - strand, within mntA at 1347.571 kb on + strand, within mntA at 1347.579 kb on - strand, within mntA at 1347.654 kb on - strand, within mntA at 1347.748 kb on + strand, within mntA at 1347.758 kb on + strand, within mntA at 1347.768 kb on + strand, within mntA at 1347.852 kb on + strand at 1347.852 kb on + strand at 1347.852 kb on + strand at 1347.852 kb on + strand at 1347.852 kb on + strand at 1347.860 kb on - strand at 1347.860 kb on - strand at 1347.860 kb on - strand at 1347.876 kb on - strand at 1347.882 kb on - strand at 1347.926 kb on - strand at 1347.949 kb on - strand at 1347.974 kb on - strand at 1348.055 kb on + strand, within mntB at 1348.070 kb on - strand, within mntB at 1348.112 kb on - strand, within mntB at 1348.118 kb on - strand, within mntB at 1348.154 kb on - strand, within mntB at 1348.177 kb on + strand, within mntB at 1348.188 kb on + strand, within mntB at 1348.251 kb on - strand, within mntB at 1348.340 kb on - strand, within mntB at 1348.352 kb on - strand, within mntB at 1348.368 kb on - strand, within mntB at 1348.390 kb on + strand, within mntB at 1348.398 kb on - strand, within mntB at 1348.413 kb on + strand, within mntB at 1348.433 kb on + strand, within mntB at 1348.448 kb on + strand, within mntB at 1348.471 kb on - strand, within mntB at 1348.518 kb on + strand, within mntB at 1348.548 kb on - strand, within mntB at 1348.549 kb on + strand, within mntB at 1348.554 kb on + strand, within mntB at 1348.554 kb on + strand, within mntB at 1348.568 kb on + strand, within mntB at 1348.573 kb on + strand, within mntB at 1348.581 kb on - strand, within mntB at 1348.586 kb on + strand, within mntB at 1348.601 kb on - strand, within mntB at 1348.617 kb on - strand, within mntB at 1348.620 kb on + strand, within mntB at 1348.644 kb on + strand, within mntB at 1348.652 kb on - strand, within mntB at 1348.673 kb on + strand, within mntB at 1348.678 kb on + strand, within mntB at 1348.738 kb on - strand at 1348.741 kb on + strand at 1348.764 kb on + strand at 1348.780 kb on - strand at 1348.794 kb on + strand at 1348.796 kb on - strand at 1348.840 kb on - strand at 1348.890 kb on - strand, within Synpcc7942_1319 at 1348.972 kb on - strand, within Synpcc7942_1319 at 1348.982 kb on - strand, within Synpcc7942_1319 at 1348.987 kb on + strand, within Synpcc7942_1319 at 1349.002 kb on - strand, within Synpcc7942_1319 at 1349.004 kb on + strand, within Synpcc7942_1319 at 1349.012 kb on - strand, within Synpcc7942_1319 at 1349.041 kb on - strand, within Synpcc7942_1319 at 1349.041 kb on - strand, within Synpcc7942_1319 at 1349.070 kb on + strand, within Synpcc7942_1319 at 1349.104 kb on + strand at 1349.104 kb on + strand, within Synpcc7942_1319 at 1349.104 kb on + strand, within Synpcc7942_1319 at 1349.104 kb on + strand, within Synpcc7942_1319 at 1349.104 kb on + strand, within Synpcc7942_1319 at 1349.104 kb on + strand, within Synpcc7942_1319 at 1349.104 kb on + strand, within Synpcc7942_1319 at 1349.112 kb on - strand, within Synpcc7942_1319 at 1349.112 kb on - strand, within Synpcc7942_1319 at 1349.112 kb on - strand, within Synpcc7942_1319 at 1349.112 kb on - strand, within Synpcc7942_1319 at 1349.112 kb on - strand, within Synpcc7942_1319 at 1349.112 kb on - strand, within Synpcc7942_1319 at 1349.112 kb on - strand, within Synpcc7942_1319 at 1349.112 kb on - strand, within Synpcc7942_1319 at 1349.112 kb on - strand, within Synpcc7942_1319 at 1349.118 kb on + strand, within Synpcc7942_1319 at 1349.130 kb on + strand, within Synpcc7942_1319 at 1349.131 kb on + strand, within Synpcc7942_1319 at 1349.134 kb on - strand, within Synpcc7942_1319 at 1349.144 kb on - strand, within Synpcc7942_1319 at 1349.148 kb on + strand, within Synpcc7942_1319 at 1349.149 kb on + strand, within Synpcc7942_1319 at 1349.184 kb on - strand, within Synpcc7942_1319 at 1349.186 kb on + strand, within Synpcc7942_1319 at 1349.222 kb on + strand, within Synpcc7942_1319 at 1349.254 kb on + strand, within Synpcc7942_1319 at 1349.259 kb on + strand, within Synpcc7942_1319 at 1349.282 kb on + strand, within Synpcc7942_1319 at 1349.293 kb on + strand, within Synpcc7942_1319 at 1349.318 kb on - strand, within Synpcc7942_1319 at 1349.326 kb on - strand at 1349.347 kb on - strand at 1349.348 kb on + strand at 1349.348 kb on + strand at 1349.353 kb on + strand at 1349.356 kb on - strand at 1349.358 kb on + strand at 1349.358 kb on + strand at 1349.366 kb on - strand at 1349.394 kb on + strand at 1349.401 kb on + strand at 1349.402 kb on - strand at 1349.402 kb on - strand at 1349.402 kb on - strand at 1349.402 kb on - strand at 1349.409 kb on - strand at 1349.414 kb on + strand at 1349.417 kb on - strand at 1349.437 kb on + strand, within Synpcc7942_1320 at 1349.447 kb on + strand, within Synpcc7942_1320 at 1349.468 kb on + strand, within Synpcc7942_1320 at 1349.498 kb on - strand, within Synpcc7942_1320 at 1349.549 kb on - strand, within Synpcc7942_1320 at 1349.557 kb on - strand, within Synpcc7942_1320 at 1349.596 kb on + strand, within Synpcc7942_1320 at 1349.596 kb on + strand, within Synpcc7942_1320 at 1349.604 kb on - strand, within Synpcc7942_1320 at 1349.604 kb on - strand, within Synpcc7942_1320 at 1349.604 kb on - strand, within Synpcc7942_1320 at 1349.604 kb on - strand, within Synpcc7942_1320 at 1349.604 kb on - strand, within Synpcc7942_1320 at 1349.625 kb on + strand, within Synpcc7942_1320 at 1349.651 kb on + strand, within Synpcc7942_1320 at 1349.659 kb on - strand, within Synpcc7942_1320 at 1349.660 kb on - strand, within Synpcc7942_1320 at 1349.660 kb on - strand, within Synpcc7942_1320 at 1349.673 kb on + strand, within Synpcc7942_1320 at 1349.680 kb on + strand, within Synpcc7942_1320 at 1349.686 kb on - strand, within Synpcc7942_1320 at 1349.688 kb on - strand, within Synpcc7942_1320 at 1349.688 kb on - strand, within Synpcc7942_1320 at 1349.688 kb on - strand, within Synpcc7942_1320 at 1349.724 kb on + strand, within Synpcc7942_1320 at 1349.735 kb on - strand, within Synpcc7942_1320 at 1349.738 kb on - strand, within Synpcc7942_1320
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown at 18C remove 1,346,943 - mntC Synpcc7942_1316 0.83 -3.7 1,346,948 - mntC Synpcc7942_1316 0.84 -1.7 1,346,975 + mntC Synpcc7942_1316 0.87 -1.4 1,346,982 + mntC Synpcc7942_1316 0.87 -4.4 1,346,988 - mntC Synpcc7942_1316 0.88 -0.9 1,346,990 + mntC Synpcc7942_1316 0.88 -2.4 1,346,990 + -0.9 1,346,998 - mntC Synpcc7942_1316 0.89 -1.8 1,347,002 + mntC Synpcc7942_1316 0.89 -1.4 1,347,002 + mntC Synpcc7942_1316 0.89 -1.6 1,347,002 + mntC Synpcc7942_1316 0.89 -1.9 1,347,002 + mntC Synpcc7942_1316 0.89 +3.4 1,347,010 - mntC Synpcc7942_1316 0.90 -0.5 1,347,010 - mntC Synpcc7942_1316 0.90 -3.5 1,347,010 - mntC Synpcc7942_1316 0.90 -1.4 1,347,010 - -1.9 1,347,010 - mntC Synpcc7942_1316 0.90 -2.4 1,347,010 - mntC Synpcc7942_1316 0.90 -0.9 1,347,020 - -1.2 1,347,020 - -2.8 1,347,038 + -0.9 1,347,044 + -1.9 1,347,044 - -1.4 1,347,062 + -2.7 1,347,075 - -0.2 1,347,075 - -2.0 1,347,075 - -0.4 1,347,080 + -2.1 1,347,091 + -0.2 1,347,103 - +2.3 1,347,117 + -2.3 1,347,125 - -0.5 1,347,125 - -2.6 1,347,125 - -0.2 1,347,127 + +0.4 1,347,190 - -2.7 1,347,250 - mntA Synpcc7942_1317 0.10 -1.2 1,347,263 + mntA Synpcc7942_1317 0.12 -2.0 1,347,263 + mntA Synpcc7942_1317 0.12 -3.8 1,347,277 + mntA Synpcc7942_1317 0.14 -2.2 1,347,311 + mntA Synpcc7942_1317 0.19 -2.6 1,347,311 + mntA Synpcc7942_1317 0.19 -2.4 1,347,319 - mntA Synpcc7942_1317 0.20 -4.3 1,347,319 - mntA Synpcc7942_1317 0.20 -2.7 1,347,322 + mntA Synpcc7942_1317 0.20 -1.5 1,347,344 + mntA Synpcc7942_1317 0.23 -2.1 1,347,354 - mntA Synpcc7942_1317 0.25 -1.8 1,347,379 + mntA Synpcc7942_1317 0.28 -3.9 1,347,379 + mntA Synpcc7942_1317 0.28 -2.6 1,347,398 - mntA Synpcc7942_1317 0.30 -2.5 1,347,424 - mntA Synpcc7942_1317 0.34 -2.6 1,347,571 + mntA Synpcc7942_1317 0.54 -3.0 1,347,579 - mntA Synpcc7942_1317 0.55 -3.4 1,347,654 - mntA Synpcc7942_1317 0.65 -2.0 1,347,748 + mntA Synpcc7942_1317 0.78 -1.4 1,347,758 + mntA Synpcc7942_1317 0.79 -0.4 1,347,768 + mntA Synpcc7942_1317 0.81 -0.3 1,347,852 + -1.4 1,347,852 + +0.1 1,347,852 + +1.1 1,347,852 + -2.1 1,347,852 + +0.2 1,347,860 - -4.2 1,347,860 - +0.6 1,347,860 - +0.8 1,347,876 - +0.7 1,347,882 - +0.1 1,347,926 - +1.0 1,347,949 - +0.3 1,347,974 - -1.2 1,348,055 + mntB Synpcc7942_1318 0.16 -1.9 1,348,070 - mntB Synpcc7942_1318 0.18 -2.2 1,348,112 - mntB Synpcc7942_1318 0.23 -1.5 1,348,118 - mntB Synpcc7942_1318 0.24 -1.9 1,348,154 - mntB Synpcc7942_1318 0.28 -1.8 1,348,177 + mntB Synpcc7942_1318 0.31 -3.1 1,348,188 + mntB Synpcc7942_1318 0.32 -1.9 1,348,251 - mntB Synpcc7942_1318 0.39 -0.2 1,348,340 - mntB Synpcc7942_1318 0.50 -2.9 1,348,352 - mntB Synpcc7942_1318 0.51 -1.6 1,348,368 - mntB Synpcc7942_1318 0.53 -3.5 1,348,390 + mntB Synpcc7942_1318 0.56 -2.2 1,348,398 - mntB Synpcc7942_1318 0.56 -1.8 1,348,413 + mntB Synpcc7942_1318 0.58 -1.1 1,348,433 + mntB Synpcc7942_1318 0.61 -3.7 1,348,448 + mntB Synpcc7942_1318 0.62 -0.7 1,348,471 - mntB Synpcc7942_1318 0.65 -3.9 1,348,518 + mntB Synpcc7942_1318 0.71 -1.2 1,348,548 - mntB Synpcc7942_1318 0.74 -3.3 1,348,549 + mntB Synpcc7942_1318 0.74 -1.9 1,348,554 + mntB Synpcc7942_1318 0.75 -1.5 1,348,554 + mntB Synpcc7942_1318 0.75 -0.8 1,348,568 + mntB Synpcc7942_1318 0.76 -1.4 1,348,573 + mntB Synpcc7942_1318 0.77 -2.0 1,348,581 - mntB Synpcc7942_1318 0.78 -0.4 1,348,586 + mntB Synpcc7942_1318 0.79 -2.8 1,348,601 - mntB Synpcc7942_1318 0.80 -2.0 1,348,617 - mntB Synpcc7942_1318 0.82 -1.4 1,348,620 + mntB Synpcc7942_1318 0.83 -1.8 1,348,644 + mntB Synpcc7942_1318 0.85 -1.4 1,348,652 - mntB Synpcc7942_1318 0.86 -3.0 1,348,673 + mntB Synpcc7942_1318 0.89 -2.3 1,348,678 + mntB Synpcc7942_1318 0.89 -1.7 1,348,738 - -0.6 1,348,741 + +2.1 1,348,764 + +0.4 1,348,780 - -0.2 1,348,794 + -1.2 1,348,796 - +1.2 1,348,840 - +1.1 1,348,890 - Synpcc7942_1319 0.10 +2.2 1,348,972 - Synpcc7942_1319 0.26 -1.9 1,348,982 - Synpcc7942_1319 0.27 +1.2 1,348,987 + Synpcc7942_1319 0.28 -1.0 1,349,002 - Synpcc7942_1319 0.31 -0.5 1,349,004 + Synpcc7942_1319 0.31 +2.0 1,349,012 - Synpcc7942_1319 0.33 +1.7 1,349,041 - Synpcc7942_1319 0.38 +0.7 1,349,041 - Synpcc7942_1319 0.38 +0.2 1,349,070 + Synpcc7942_1319 0.44 +0.1 1,349,104 + -0.6 1,349,104 + Synpcc7942_1319 0.50 +3.3 1,349,104 + Synpcc7942_1319 0.50 -0.7 1,349,104 + Synpcc7942_1319 0.50 -1.1 1,349,104 + Synpcc7942_1319 0.50 -1.3 1,349,104 + Synpcc7942_1319 0.50 -2.8 1,349,104 + Synpcc7942_1319 0.50 +0.8 1,349,112 - Synpcc7942_1319 0.51 -3.8 1,349,112 - Synpcc7942_1319 0.51 -0.7 1,349,112 - Synpcc7942_1319 0.51 -2.9 1,349,112 - Synpcc7942_1319 0.51 +3.2 1,349,112 - Synpcc7942_1319 0.51 +0.8 1,349,112 - Synpcc7942_1319 0.51 +1.0 1,349,112 - Synpcc7942_1319 0.51 -0.3 1,349,112 - Synpcc7942_1319 0.51 +0.6 1,349,112 - Synpcc7942_1319 0.51 -0.7 1,349,118 + Synpcc7942_1319 0.53 -1.7 1,349,130 + Synpcc7942_1319 0.55 +0.9 1,349,131 + Synpcc7942_1319 0.55 +1.8 1,349,134 - Synpcc7942_1319 0.56 +0.5 1,349,144 - Synpcc7942_1319 0.57 -1.7 1,349,148 + Synpcc7942_1319 0.58 +2.2 1,349,149 + Synpcc7942_1319 0.58 +1.7 1,349,184 - Synpcc7942_1319 0.65 +0.9 1,349,186 + Synpcc7942_1319 0.65 +1.6 1,349,222 + Synpcc7942_1319 0.72 +0.0 1,349,254 + Synpcc7942_1319 0.78 +0.0 1,349,259 + Synpcc7942_1319 0.79 -1.4 1,349,282 + Synpcc7942_1319 0.83 -3.3 1,349,293 + Synpcc7942_1319 0.85 +0.7 1,349,318 - Synpcc7942_1319 0.90 +2.3 1,349,326 - -1.7 1,349,347 - -1.9 1,349,348 + +1.1 1,349,348 + +1.4 1,349,353 + +1.8 1,349,356 - +0.4 1,349,358 + -1.5 1,349,358 + +1.5 1,349,366 - +0.1 1,349,394 + -1.0 1,349,401 + +1.3 1,349,402 - +1.8 1,349,402 - +0.6 1,349,402 - -0.3 1,349,402 - +0.1 1,349,409 - -2.1 1,349,414 + +0.7 1,349,417 - +0.1 1,349,437 + Synpcc7942_1320 0.10 +1.1 1,349,447 + Synpcc7942_1320 0.11 +0.1 1,349,468 + Synpcc7942_1320 0.13 +0.7 1,349,498 - Synpcc7942_1320 0.17 -2.3 1,349,549 - Synpcc7942_1320 0.22 -1.6 1,349,557 - Synpcc7942_1320 0.23 -1.3 1,349,596 + Synpcc7942_1320 0.27 -2.7 1,349,596 + Synpcc7942_1320 0.27 -0.4 1,349,604 - Synpcc7942_1320 0.28 -4.1 1,349,604 - Synpcc7942_1320 0.28 +0.1 1,349,604 - Synpcc7942_1320 0.28 +0.8 1,349,604 - Synpcc7942_1320 0.28 -0.4 1,349,604 - Synpcc7942_1320 0.28 -2.1 1,349,625 + Synpcc7942_1320 0.30 +0.1 1,349,651 + Synpcc7942_1320 0.32 -1.4 1,349,659 - Synpcc7942_1320 0.33 +0.9 1,349,660 - Synpcc7942_1320 0.33 -2.4 1,349,660 - Synpcc7942_1320 0.33 -2.6 1,349,673 + Synpcc7942_1320 0.35 +0.7 1,349,680 + Synpcc7942_1320 0.36 -1.4 1,349,686 - Synpcc7942_1320 0.36 +0.9 1,349,688 - Synpcc7942_1320 0.36 -0.5 1,349,688 - Synpcc7942_1320 0.36 -2.4 1,349,688 - Synpcc7942_1320 0.36 +0.3 1,349,724 + Synpcc7942_1320 0.40 -2.8 1,349,735 - Synpcc7942_1320 0.41 -2.5 1,349,738 - Synpcc7942_1320 0.42 +1.1
Or see this region's nucleotide sequence