Experiment: Flask grown at 18C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_1113 and rbgA are separated by 6 nucleotides rbgA and Synpcc7942_1115 overlap by 4 nucleotides Synpcc7942_1115 and pgk are separated by 247 nucleotides
Synpcc7942_1113: Synpcc7942_1113 - hypothetical protein, at 1,130,382 to 1,131,473
_1113
Synpcc7942_1114: rbgA - ribosomal biogenesis GTPase, at 1,131,480 to 1,132,343
rbgA
Synpcc7942_1115: Synpcc7942_1115 - hypothetical protein, at 1,132,340 to 1,132,720
_1115
Synpcc7942_1116: pgk - phosphoglycerate kinase, at 1,132,968 to 1,134,176
pgk
Position (kb)
1132
1133 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 1131.355 kb on + strand, within Synpcc7942_1113 at 1131.363 kb on - strand, within Synpcc7942_1113 at 1131.376 kb on - strand at 1131.376 kb on - strand at 1131.399 kb on - strand at 1131.401 kb on + strand at 1131.413 kb on + strand at 1131.439 kb on + strand at 1131.439 kb on + strand at 1131.471 kb on + strand at 1131.479 kb on - strand at 1131.485 kb on - strand at 1132.344 kb on - strand at 1132.359 kb on + strand at 1132.359 kb on + strand at 1132.359 kb on + strand at 1132.359 kb on + strand at 1132.367 kb on - strand at 1132.389 kb on - strand, within Synpcc7942_1115 at 1132.450 kb on + strand, within Synpcc7942_1115 at 1132.458 kb on - strand, within Synpcc7942_1115 at 1132.465 kb on + strand, within Synpcc7942_1115 at 1132.470 kb on - strand, within Synpcc7942_1115 at 1132.490 kb on + strand, within Synpcc7942_1115 at 1132.498 kb on - strand, within Synpcc7942_1115 at 1132.498 kb on - strand, within Synpcc7942_1115 at 1132.499 kb on + strand, within Synpcc7942_1115 at 1132.529 kb on + strand, within Synpcc7942_1115 at 1132.567 kb on + strand, within Synpcc7942_1115 at 1132.575 kb on - strand, within Synpcc7942_1115 at 1132.575 kb on - strand, within Synpcc7942_1115 at 1132.575 kb on - strand, within Synpcc7942_1115 at 1132.575 kb on - strand, within Synpcc7942_1115 at 1132.580 kb on - strand, within Synpcc7942_1115 at 1132.580 kb on - strand, within Synpcc7942_1115 at 1132.613 kb on + strand, within Synpcc7942_1115 at 1132.621 kb on - strand, within Synpcc7942_1115 at 1132.632 kb on + strand, within Synpcc7942_1115 at 1132.649 kb on + strand, within Synpcc7942_1115 at 1132.649 kb on + strand, within Synpcc7942_1115 at 1132.657 kb on - strand, within Synpcc7942_1115 at 1132.657 kb on - strand, within Synpcc7942_1115 at 1132.658 kb on - strand, within Synpcc7942_1115 at 1132.676 kb on - strand, within Synpcc7942_1115 at 1132.677 kb on - strand, within Synpcc7942_1115 at 1132.678 kb on - strand, within Synpcc7942_1115 at 1132.678 kb on - strand, within Synpcc7942_1115 at 1132.729 kb on + strand at 1132.747 kb on - strand at 1132.747 kb on - strand at 1132.748 kb on + strand at 1132.756 kb on - strand at 1132.756 kb on - strand at 1132.913 kb on - strand at 1132.954 kb on - strand at 1132.954 kb on - strand at 1132.954 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown at 18C remove 1,131,355 + Synpcc7942_1113 0.89 -2.6 1,131,363 - Synpcc7942_1113 0.90 -1.1 1,131,376 - +1.4 1,131,376 - -0.5 1,131,399 - +3.2 1,131,401 + -0.1 1,131,413 + +0.0 1,131,439 + +1.0 1,131,439 + +1.4 1,131,471 + -2.3 1,131,479 - -1.1 1,131,485 - +0.9 1,132,344 - +2.0 1,132,359 + +3.4 1,132,359 + -2.6 1,132,359 + +1.6 1,132,359 + +1.2 1,132,367 - +3.2 1,132,389 - Synpcc7942_1115 0.13 -2.1 1,132,450 + Synpcc7942_1115 0.29 +0.6 1,132,458 - Synpcc7942_1115 0.31 +0.5 1,132,465 + Synpcc7942_1115 0.33 +3.5 1,132,470 - Synpcc7942_1115 0.34 +2.5 1,132,490 + Synpcc7942_1115 0.39 +1.7 1,132,498 - Synpcc7942_1115 0.41 -1.4 1,132,498 - Synpcc7942_1115 0.41 -1.1 1,132,499 + Synpcc7942_1115 0.42 -0.2 1,132,529 + Synpcc7942_1115 0.50 -0.3 1,132,567 + Synpcc7942_1115 0.60 +1.9 1,132,575 - Synpcc7942_1115 0.62 -3.1 1,132,575 - Synpcc7942_1115 0.62 -0.7 1,132,575 - Synpcc7942_1115 0.62 +3.6 1,132,575 - Synpcc7942_1115 0.62 +2.9 1,132,580 - Synpcc7942_1115 0.63 +1.9 1,132,580 - Synpcc7942_1115 0.63 +1.4 1,132,613 + Synpcc7942_1115 0.72 +1.9 1,132,621 - Synpcc7942_1115 0.74 +2.9 1,132,632 + Synpcc7942_1115 0.77 +2.6 1,132,649 + Synpcc7942_1115 0.81 -1.2 1,132,649 + Synpcc7942_1115 0.81 +1.8 1,132,657 - Synpcc7942_1115 0.83 +1.6 1,132,657 - Synpcc7942_1115 0.83 +2.7 1,132,658 - Synpcc7942_1115 0.83 +5.1 1,132,676 - Synpcc7942_1115 0.88 +3.3 1,132,677 - Synpcc7942_1115 0.88 +3.5 1,132,678 - Synpcc7942_1115 0.89 +3.3 1,132,678 - Synpcc7942_1115 0.89 +4.6 1,132,729 + +1.4 1,132,747 - -1.5 1,132,747 - +0.5 1,132,748 + +1.1 1,132,756 - +1.2 1,132,756 - +0.2 1,132,913 - +5.0 1,132,954 - +3.6 1,132,954 - +2.8 1,132,954 - +3.2
Or see this region's nucleotide sequence