Experiment: Flask grown at 18C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thiC and Synpcc7942_1097 are separated by 97 nucleotides Synpcc7942_1097 and Synpcc7942_1098 are separated by 72 nucleotides Synpcc7942_1098 and ubiE are separated by 2 nucleotides ubiE and dnaA are separated by 140 nucleotides
Synpcc7942_1096: thiC - thiamine biosynthesis protein ThiC, at 1,113,354 to 1,114,724
thiC
Synpcc7942_1097: Synpcc7942_1097 - hypothetical protein, at 1,114,822 to 1,114,947
_1097
Synpcc7942_1098: Synpcc7942_1098 - hypothetical protein, at 1,115,020 to 1,116,258
_1098
Synpcc7942_1099: ubiE - ubiquinone/menaquinone biosynthesis methyltransferase, at 1,116,261 to 1,116,962
ubiE
Synpcc7942_1100: dnaA - chromosomal replication initiation protein, at 1,117,103 to 1,118,551
dnaA
Position (kb)
1115
1116
1117 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 1114.948 kb on - strand at 1114.958 kb on + strand at 1114.971 kb on - strand at 1114.971 kb on - strand at 1114.971 kb on - strand at 1115.006 kb on + strand at 1115.006 kb on + strand at 1115.020 kb on - strand at 1115.024 kb on - strand at 1115.049 kb on + strand at 1115.050 kb on + strand at 1115.062 kb on + strand at 1115.062 kb on + strand at 1115.062 kb on + strand at 1115.062 kb on + strand at 1115.065 kb on + strand at 1115.070 kb on - strand at 1115.070 kb on - strand at 1115.070 kb on - strand at 1115.070 kb on - strand at 1115.070 kb on - strand at 1115.070 kb on - strand at 1115.075 kb on - strand at 1115.092 kb on + strand at 1115.118 kb on + strand at 1115.174 kb on + strand, within Synpcc7942_1098 at 1115.208 kb on + strand, within Synpcc7942_1098 at 1115.218 kb on + strand, within Synpcc7942_1098 at 1115.299 kb on + strand, within Synpcc7942_1098 at 1115.299 kb on + strand, within Synpcc7942_1098 at 1115.299 kb on + strand, within Synpcc7942_1098 at 1115.302 kb on + strand, within Synpcc7942_1098 at 1115.304 kb on + strand, within Synpcc7942_1098 at 1115.305 kb on - strand, within Synpcc7942_1098 at 1115.307 kb on + strand, within Synpcc7942_1098 at 1115.335 kb on - strand, within Synpcc7942_1098 at 1115.348 kb on + strand, within Synpcc7942_1098 at 1115.348 kb on + strand, within Synpcc7942_1098 at 1115.372 kb on + strand, within Synpcc7942_1098 at 1115.415 kb on + strand, within Synpcc7942_1098 at 1115.425 kb on - strand, within Synpcc7942_1098 at 1115.427 kb on + strand, within Synpcc7942_1098 at 1115.427 kb on + strand, within Synpcc7942_1098 at 1115.427 kb on + strand, within Synpcc7942_1098 at 1115.435 kb on - strand, within Synpcc7942_1098 at 1115.439 kb on + strand, within Synpcc7942_1098 at 1115.456 kb on - strand, within Synpcc7942_1098 at 1115.472 kb on + strand, within Synpcc7942_1098 at 1115.472 kb on + strand, within Synpcc7942_1098 at 1115.472 kb on + strand, within Synpcc7942_1098 at 1115.472 kb on + strand, within Synpcc7942_1098 at 1115.472 kb on + strand, within Synpcc7942_1098 at 1115.472 kb on + strand, within Synpcc7942_1098 at 1115.480 kb on - strand, within Synpcc7942_1098 at 1115.480 kb on - strand, within Synpcc7942_1098 at 1115.502 kb on - strand, within Synpcc7942_1098 at 1115.517 kb on + strand, within Synpcc7942_1098 at 1115.517 kb on + strand, within Synpcc7942_1098 at 1115.527 kb on - strand, within Synpcc7942_1098 at 1115.552 kb on + strand, within Synpcc7942_1098 at 1115.552 kb on + strand, within Synpcc7942_1098 at 1115.552 kb on + strand, within Synpcc7942_1098 at 1115.559 kb on - strand, within Synpcc7942_1098 at 1115.591 kb on + strand, within Synpcc7942_1098 at 1115.595 kb on - strand, within Synpcc7942_1098 at 1115.599 kb on - strand, within Synpcc7942_1098 at 1115.600 kb on - strand, within Synpcc7942_1098 at 1115.666 kb on + strand, within Synpcc7942_1098 at 1115.666 kb on + strand, within Synpcc7942_1098 at 1115.731 kb on - strand, within Synpcc7942_1098 at 1115.747 kb on - strand, within Synpcc7942_1098 at 1115.757 kb on + strand, within Synpcc7942_1098 at 1115.761 kb on - strand, within Synpcc7942_1098 at 1115.789 kb on - strand, within Synpcc7942_1098 at 1115.789 kb on - strand, within Synpcc7942_1098 at 1115.810 kb on - strand, within Synpcc7942_1098 at 1115.810 kb on - strand, within Synpcc7942_1098 at 1115.826 kb on + strand, within Synpcc7942_1098 at 1115.827 kb on + strand, within Synpcc7942_1098 at 1115.827 kb on + strand, within Synpcc7942_1098 at 1115.827 kb on + strand, within Synpcc7942_1098 at 1115.835 kb on - strand, within Synpcc7942_1098 at 1115.835 kb on - strand, within Synpcc7942_1098 at 1115.835 kb on - strand, within Synpcc7942_1098 at 1115.835 kb on - strand, within Synpcc7942_1098 at 1115.856 kb on + strand, within Synpcc7942_1098 at 1115.856 kb on + strand, within Synpcc7942_1098 at 1115.856 kb on + strand, within Synpcc7942_1098 at 1115.856 kb on + strand, within Synpcc7942_1098 at 1115.869 kb on + strand, within Synpcc7942_1098 at 1115.877 kb on - strand, within Synpcc7942_1098 at 1115.877 kb on - strand, within Synpcc7942_1098 at 1115.877 kb on - strand, within Synpcc7942_1098 at 1115.901 kb on + strand, within Synpcc7942_1098 at 1115.901 kb on - strand, within Synpcc7942_1098 at 1115.901 kb on - strand, within Synpcc7942_1098 at 1115.913 kb on + strand, within Synpcc7942_1098 at 1115.913 kb on + strand, within Synpcc7942_1098 at 1115.921 kb on - strand, within Synpcc7942_1098 at 1116.020 kb on + strand, within Synpcc7942_1098 at 1116.033 kb on + strand, within Synpcc7942_1098 at 1116.033 kb on + strand, within Synpcc7942_1098 at 1116.033 kb on + strand, within Synpcc7942_1098 at 1116.033 kb on + strand, within Synpcc7942_1098 at 1116.033 kb on + strand, within Synpcc7942_1098 at 1116.041 kb on - strand, within Synpcc7942_1098 at 1116.041 kb on - strand, within Synpcc7942_1098 at 1116.041 kb on - strand, within Synpcc7942_1098 at 1116.041 kb on - strand, within Synpcc7942_1098 at 1116.041 kb on - strand, within Synpcc7942_1098 at 1116.041 kb on - strand, within Synpcc7942_1098 at 1116.041 kb on - strand, within Synpcc7942_1098 at 1116.041 kb on - strand, within Synpcc7942_1098 at 1116.041 kb on - strand, within Synpcc7942_1098 at 1116.075 kb on + strand, within Synpcc7942_1098 at 1116.096 kb on - strand, within Synpcc7942_1098 at 1116.116 kb on - strand, within Synpcc7942_1098 at 1116.139 kb on + strand at 1116.147 kb on - strand at 1116.231 kb on + strand at 1116.231 kb on + strand at 1116.231 kb on + strand at 1116.231 kb on + strand at 1116.231 kb on + strand at 1116.266 kb on + strand at 1116.273 kb on + strand at 1116.280 kb on + strand at 1116.290 kb on + strand at 1116.293 kb on - strand at 1116.298 kb on - strand at 1116.298 kb on - strand at 1116.318 kb on - strand at 1116.353 kb on + strand, within ubiE at 1116.360 kb on + strand, within ubiE at 1116.365 kb on + strand, within ubiE at 1116.369 kb on - strand, within ubiE at 1116.382 kb on - strand, within ubiE at 1116.409 kb on + strand, within ubiE at 1116.409 kb on + strand, within ubiE at 1116.414 kb on + strand, within ubiE at 1116.422 kb on - strand, within ubiE at 1116.422 kb on - strand, within ubiE at 1116.459 kb on - strand, within ubiE at 1116.459 kb on - strand, within ubiE at 1116.470 kb on + strand, within ubiE at 1116.484 kb on + strand, within ubiE at 1116.492 kb on - strand, within ubiE at 1116.523 kb on - strand, within ubiE at 1116.528 kb on + strand, within ubiE at 1116.529 kb on + strand, within ubiE at 1116.582 kb on - strand, within ubiE at 1116.584 kb on + strand, within ubiE at 1116.584 kb on + strand, within ubiE at 1116.584 kb on + strand, within ubiE at 1116.584 kb on + strand, within ubiE at 1116.584 kb on + strand, within ubiE at 1116.584 kb on + strand, within ubiE at 1116.584 kb on + strand, within ubiE at 1116.584 kb on + strand, within ubiE at 1116.584 kb on + strand, within ubiE at 1116.592 kb on - strand, within ubiE at 1116.592 kb on - strand, within ubiE at 1116.592 kb on - strand, within ubiE at 1116.592 kb on - strand, within ubiE at 1116.592 kb on - strand, within ubiE at 1116.592 kb on - strand, within ubiE at 1116.593 kb on - strand, within ubiE at 1116.604 kb on + strand, within ubiE at 1116.622 kb on + strand, within ubiE at 1116.636 kb on - strand, within ubiE at 1116.641 kb on - strand, within ubiE at 1116.641 kb on - strand, within ubiE at 1116.673 kb on - strand, within ubiE at 1116.676 kb on - strand, within ubiE at 1116.678 kb on + strand, within ubiE at 1116.726 kb on + strand, within ubiE at 1116.738 kb on + strand, within ubiE at 1116.793 kb on + strand, within ubiE at 1116.837 kb on + strand, within ubiE at 1116.845 kb on - strand, within ubiE at 1116.847 kb on + strand, within ubiE at 1116.877 kb on + strand, within ubiE at 1116.889 kb on + strand, within ubiE at 1116.900 kb on + strand at 1116.930 kb on + strand at 1116.951 kb on - strand at 1116.953 kb on + strand at 1116.958 kb on + strand at 1116.968 kb on - strand at 1116.974 kb on - strand at 1116.974 kb on - strand at 1116.989 kb on + strand at 1116.990 kb on - strand at 1117.030 kb on + strand at 1117.030 kb on + strand at 1117.047 kb on + strand at 1117.058 kb on + strand at 1117.058 kb on + strand at 1117.068 kb on - strand at 1117.079 kb on + strand at 1117.124 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown at 18C remove 1,114,948 - -0.6 1,114,958 + +1.5 1,114,971 - -3.0 1,114,971 - +0.5 1,114,971 - +2.9 1,115,006 + +2.2 1,115,006 + -3.8 1,115,020 - -0.1 1,115,024 - +1.0 1,115,049 + -0.3 1,115,050 + -0.9 1,115,062 + +0.2 1,115,062 + -1.9 1,115,062 + -1.1 1,115,062 + -2.3 1,115,065 + +1.4 1,115,070 - -1.0 1,115,070 - +1.1 1,115,070 - +1.0 1,115,070 - -0.5 1,115,070 - -3.6 1,115,070 - -0.8 1,115,075 - -1.3 1,115,092 + -4.1 1,115,118 + -1.2 1,115,174 + Synpcc7942_1098 0.12 -2.2 1,115,208 + Synpcc7942_1098 0.15 -0.3 1,115,218 + Synpcc7942_1098 0.16 -1.2 1,115,299 + Synpcc7942_1098 0.23 +0.2 1,115,299 + Synpcc7942_1098 0.23 -0.6 1,115,299 + Synpcc7942_1098 0.23 -3.2 1,115,302 + Synpcc7942_1098 0.23 -4.3 1,115,304 + Synpcc7942_1098 0.23 -0.1 1,115,305 - Synpcc7942_1098 0.23 -3.6 1,115,307 + Synpcc7942_1098 0.23 -1.7 1,115,335 - Synpcc7942_1098 0.25 -1.1 1,115,348 + Synpcc7942_1098 0.26 -2.8 1,115,348 + Synpcc7942_1098 0.26 -1.3 1,115,372 + Synpcc7942_1098 0.28 -3.7 1,115,415 + Synpcc7942_1098 0.32 -0.6 1,115,425 - Synpcc7942_1098 0.33 +0.1 1,115,427 + Synpcc7942_1098 0.33 -2.3 1,115,427 + Synpcc7942_1098 0.33 -0.1 1,115,427 + Synpcc7942_1098 0.33 -2.8 1,115,435 - Synpcc7942_1098 0.33 -0.3 1,115,439 + Synpcc7942_1098 0.34 -0.5 1,115,456 - Synpcc7942_1098 0.35 -1.1 1,115,472 + Synpcc7942_1098 0.36 -5.2 1,115,472 + Synpcc7942_1098 0.36 -2.8 1,115,472 + Synpcc7942_1098 0.36 -0.3 1,115,472 + Synpcc7942_1098 0.36 -2.1 1,115,472 + Synpcc7942_1098 0.36 -3.3 1,115,472 + Synpcc7942_1098 0.36 +0.0 1,115,480 - Synpcc7942_1098 0.37 -4.2 1,115,480 - Synpcc7942_1098 0.37 -3.1 1,115,502 - Synpcc7942_1098 0.39 -2.7 1,115,517 + Synpcc7942_1098 0.40 -0.9 1,115,517 + Synpcc7942_1098 0.40 -0.3 1,115,527 - Synpcc7942_1098 0.41 -0.8 1,115,552 + Synpcc7942_1098 0.43 -0.6 1,115,552 + Synpcc7942_1098 0.43 -1.1 1,115,552 + Synpcc7942_1098 0.43 -1.8 1,115,559 - Synpcc7942_1098 0.44 -0.5 1,115,591 + Synpcc7942_1098 0.46 -0.5 1,115,595 - Synpcc7942_1098 0.46 +0.7 1,115,599 - Synpcc7942_1098 0.47 -0.9 1,115,600 - Synpcc7942_1098 0.47 -1.4 1,115,666 + Synpcc7942_1098 0.52 -3.6 1,115,666 + Synpcc7942_1098 0.52 +0.1 1,115,731 - Synpcc7942_1098 0.57 -0.7 1,115,747 - Synpcc7942_1098 0.59 +0.1 1,115,757 + Synpcc7942_1098 0.59 -1.5 1,115,761 - Synpcc7942_1098 0.60 +0.2 1,115,789 - Synpcc7942_1098 0.62 +0.1 1,115,789 - Synpcc7942_1098 0.62 +0.1 1,115,810 - Synpcc7942_1098 0.64 -2.4 1,115,810 - Synpcc7942_1098 0.64 -4.5 1,115,826 + Synpcc7942_1098 0.65 -0.7 1,115,827 + Synpcc7942_1098 0.65 -1.2 1,115,827 + Synpcc7942_1098 0.65 -0.8 1,115,827 + Synpcc7942_1098 0.65 -1.8 1,115,835 - Synpcc7942_1098 0.66 +0.8 1,115,835 - Synpcc7942_1098 0.66 -1.9 1,115,835 - Synpcc7942_1098 0.66 -2.2 1,115,835 - Synpcc7942_1098 0.66 -0.2 1,115,856 + Synpcc7942_1098 0.67 -1.5 1,115,856 + Synpcc7942_1098 0.67 -1.3 1,115,856 + Synpcc7942_1098 0.67 +0.1 1,115,856 + Synpcc7942_1098 0.67 +1.3 1,115,869 + Synpcc7942_1098 0.69 -0.3 1,115,877 - Synpcc7942_1098 0.69 -2.6 1,115,877 - Synpcc7942_1098 0.69 -2.1 1,115,877 - Synpcc7942_1098 0.69 +2.1 1,115,901 + Synpcc7942_1098 0.71 -1.6 1,115,901 - Synpcc7942_1098 0.71 -2.8 1,115,901 - Synpcc7942_1098 0.71 -0.6 1,115,913 + Synpcc7942_1098 0.72 -0.4 1,115,913 + Synpcc7942_1098 0.72 -0.5 1,115,921 - Synpcc7942_1098 0.73 -2.2 1,116,020 + Synpcc7942_1098 0.81 +0.1 1,116,033 + Synpcc7942_1098 0.82 +2.8 1,116,033 + Synpcc7942_1098 0.82 -0.9 1,116,033 + Synpcc7942_1098 0.82 -0.7 1,116,033 + Synpcc7942_1098 0.82 +0.3 1,116,033 + Synpcc7942_1098 0.82 -0.7 1,116,041 - Synpcc7942_1098 0.82 -1.5 1,116,041 - Synpcc7942_1098 0.82 -2.9 1,116,041 - Synpcc7942_1098 0.82 -2.9 1,116,041 - Synpcc7942_1098 0.82 +0.2 1,116,041 - Synpcc7942_1098 0.82 +1.5 1,116,041 - Synpcc7942_1098 0.82 -0.5 1,116,041 - Synpcc7942_1098 0.82 -0.6 1,116,041 - Synpcc7942_1098 0.82 -0.5 1,116,041 - Synpcc7942_1098 0.82 -0.0 1,116,075 + Synpcc7942_1098 0.85 -1.6 1,116,096 - Synpcc7942_1098 0.87 -0.8 1,116,116 - Synpcc7942_1098 0.88 -4.3 1,116,139 + +1.5 1,116,147 - -2.9 1,116,231 + -0.7 1,116,231 + -3.3 1,116,231 + -1.0 1,116,231 + -1.4 1,116,231 + -0.8 1,116,266 + +2.3 1,116,273 + +1.3 1,116,280 + -0.5 1,116,290 + +1.8 1,116,293 - +2.0 1,116,298 - +1.0 1,116,298 - +2.2 1,116,318 - +0.8 1,116,353 + ubiE Synpcc7942_1099 0.13 +1.8 1,116,360 + ubiE Synpcc7942_1099 0.14 +3.2 1,116,365 + ubiE Synpcc7942_1099 0.15 +0.6 1,116,369 - ubiE Synpcc7942_1099 0.15 -0.6 1,116,382 - ubiE Synpcc7942_1099 0.17 +1.8 1,116,409 + ubiE Synpcc7942_1099 0.21 +1.1 1,116,409 + ubiE Synpcc7942_1099 0.21 +1.2 1,116,414 + ubiE Synpcc7942_1099 0.22 +0.6 1,116,422 - ubiE Synpcc7942_1099 0.23 +1.1 1,116,422 - ubiE Synpcc7942_1099 0.23 -5.7 1,116,459 - ubiE Synpcc7942_1099 0.28 +1.7 1,116,459 - ubiE Synpcc7942_1099 0.28 -0.2 1,116,470 + ubiE Synpcc7942_1099 0.30 +1.7 1,116,484 + ubiE Synpcc7942_1099 0.32 -3.9 1,116,492 - ubiE Synpcc7942_1099 0.33 +2.1 1,116,523 - ubiE Synpcc7942_1099 0.37 -2.3 1,116,528 + ubiE Synpcc7942_1099 0.38 -1.3 1,116,529 + ubiE Synpcc7942_1099 0.38 +3.2 1,116,582 - ubiE Synpcc7942_1099 0.46 +0.3 1,116,584 + ubiE Synpcc7942_1099 0.46 +2.5 1,116,584 + ubiE Synpcc7942_1099 0.46 -1.6 1,116,584 + ubiE Synpcc7942_1099 0.46 -0.1 1,116,584 + ubiE Synpcc7942_1099 0.46 +0.5 1,116,584 + ubiE Synpcc7942_1099 0.46 +2.2 1,116,584 + ubiE Synpcc7942_1099 0.46 -1.8 1,116,584 + ubiE Synpcc7942_1099 0.46 -1.1 1,116,584 + ubiE Synpcc7942_1099 0.46 -1.6 1,116,584 + ubiE Synpcc7942_1099 0.46 +1.1 1,116,592 - ubiE Synpcc7942_1099 0.47 -0.0 1,116,592 - ubiE Synpcc7942_1099 0.47 +2.3 1,116,592 - ubiE Synpcc7942_1099 0.47 +1.7 1,116,592 - ubiE Synpcc7942_1099 0.47 -1.1 1,116,592 - ubiE Synpcc7942_1099 0.47 -0.7 1,116,592 - ubiE Synpcc7942_1099 0.47 +0.3 1,116,593 - ubiE Synpcc7942_1099 0.47 -1.8 1,116,604 + ubiE Synpcc7942_1099 0.49 +2.7 1,116,622 + ubiE Synpcc7942_1099 0.51 -0.2 1,116,636 - ubiE Synpcc7942_1099 0.53 +1.4 1,116,641 - ubiE Synpcc7942_1099 0.54 +0.4 1,116,641 - ubiE Synpcc7942_1099 0.54 -2.9 1,116,673 - ubiE Synpcc7942_1099 0.59 +1.6 1,116,676 - ubiE Synpcc7942_1099 0.59 +0.3 1,116,678 + ubiE Synpcc7942_1099 0.59 +2.0 1,116,726 + ubiE Synpcc7942_1099 0.66 +1.6 1,116,738 + ubiE Synpcc7942_1099 0.68 +0.5 1,116,793 + ubiE Synpcc7942_1099 0.76 +2.4 1,116,837 + ubiE Synpcc7942_1099 0.82 -1.7 1,116,845 - ubiE Synpcc7942_1099 0.83 -0.1 1,116,847 + ubiE Synpcc7942_1099 0.83 -1.8 1,116,877 + ubiE Synpcc7942_1099 0.88 +1.8 1,116,889 + ubiE Synpcc7942_1099 0.89 +2.1 1,116,900 + -1.8 1,116,930 + -1.3 1,116,951 - -0.2 1,116,953 + +0.6 1,116,958 + -0.2 1,116,968 - -2.6 1,116,974 - +1.1 1,116,974 - +1.7 1,116,989 + -2.7 1,116,990 - -0.4 1,117,030 + +1.4 1,117,030 + -1.1 1,117,047 + +1.0 1,117,058 + -2.1 1,117,058 + -2.3 1,117,068 - +1.4 1,117,079 + +1.7 1,117,124 - -1.1
Or see this region's nucleotide sequence