Experiment: Flask grown at 18C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fur and ycf54 are separated by 224 nucleotides ycf54 and Synpcc7942_0989 are separated by 104 nucleotides Synpcc7942_0989 and cobU are separated by 89 nucleotides cobU and pxcA are separated by 9 nucleotides
Synpcc7942_0987: fur - putative ferric uptake regulator, FUR family, at 996,280 to 996,723
fur
Synpcc7942_0988: ycf54 - conserved hypothetical protein YCF54, at 996,948 to 997,274
ycf54
Synpcc7942_0989: Synpcc7942_0989 - hypothetical protein, at 997,379 to 997,822
_0989
Synpcc7942_0990: cobU - adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase, at 997,912 to 998,454
cobU
Synpcc7942_0991: pxcA - proton extrusion protein PcxA, at 998,464 to 999,729
pxcA
Position (kb) 997
998 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 996.769 kb on + strand at 996.802 kb on - strand at 996.804 kb on + strand at 996.804 kb on + strand at 996.822 kb on - strand at 996.841 kb on + strand at 996.841 kb on + strand at 996.849 kb on - strand at 996.849 kb on - strand at 996.849 kb on - strand at 996.849 kb on - strand at 996.851 kb on + strand at 996.851 kb on + strand at 996.882 kb on - strand at 996.917 kb on + strand at 996.917 kb on + strand at 996.917 kb on + strand at 996.917 kb on - strand at 996.917 kb on - strand at 996.925 kb on - strand at 996.925 kb on - strand at 996.953 kb on - strand at 997.325 kb on + strand at 997.333 kb on - strand at 997.393 kb on - strand at 997.458 kb on - strand, within Synpcc7942_0989 at 997.459 kb on - strand, within Synpcc7942_0989 at 997.473 kb on + strand, within Synpcc7942_0989 at 997.507 kb on + strand, within Synpcc7942_0989 at 997.515 kb on - strand, within Synpcc7942_0989 at 997.518 kb on + strand at 997.526 kb on - strand, within Synpcc7942_0989 at 997.529 kb on + strand, within Synpcc7942_0989 at 997.529 kb on + strand, within Synpcc7942_0989 at 997.531 kb on - strand, within Synpcc7942_0989 at 997.537 kb on - strand, within Synpcc7942_0989 at 997.537 kb on - strand, within Synpcc7942_0989 at 997.561 kb on + strand, within Synpcc7942_0989 at 997.561 kb on + strand, within Synpcc7942_0989 at 997.561 kb on + strand, within Synpcc7942_0989 at 997.561 kb on + strand, within Synpcc7942_0989 at 997.569 kb on - strand, within Synpcc7942_0989 at 997.569 kb on - strand, within Synpcc7942_0989 at 997.569 kb on - strand, within Synpcc7942_0989 at 997.569 kb on - strand, within Synpcc7942_0989 at 997.580 kb on + strand, within Synpcc7942_0989 at 997.582 kb on - strand, within Synpcc7942_0989 at 997.599 kb on + strand, within Synpcc7942_0989 at 997.607 kb on - strand, within Synpcc7942_0989 at 997.607 kb on - strand, within Synpcc7942_0989 at 997.607 kb on - strand at 997.626 kb on + strand, within Synpcc7942_0989 at 997.652 kb on - strand, within Synpcc7942_0989 at 997.695 kb on + strand, within Synpcc7942_0989 at 997.700 kb on + strand, within Synpcc7942_0989 at 997.703 kb on - strand, within Synpcc7942_0989 at 997.723 kb on + strand, within Synpcc7942_0989 at 997.731 kb on + strand, within Synpcc7942_0989 at 997.731 kb on + strand, within Synpcc7942_0989 at 997.731 kb on + strand, within Synpcc7942_0989 at 997.744 kb on - strand, within Synpcc7942_0989 at 997.766 kb on + strand, within Synpcc7942_0989 at 997.774 kb on + strand, within Synpcc7942_0989 at 997.776 kb on - strand, within Synpcc7942_0989 at 997.820 kb on - strand at 997.843 kb on + strand at 998.452 kb on - strand at 998.642 kb on + strand, within pxcA at 998.647 kb on + strand, within pxcA at 998.647 kb on + strand, within pxcA at 998.653 kb on + strand, within pxcA
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown at 18C remove 996,769 + +1.7 996,802 - +3.2 996,804 + -0.4 996,804 + +0.4 996,822 - +1.4 996,841 + -0.5 996,841 + -2.6 996,849 - +1.1 996,849 - +0.7 996,849 - -1.8 996,849 - -0.3 996,851 + -2.3 996,851 + +2.0 996,882 - +1.1 996,917 + +0.2 996,917 + +2.0 996,917 + +4.5 996,917 - -1.2 996,917 - -0.1 996,925 - +0.4 996,925 - -0.1 996,953 - +2.2 997,325 + +1.3 997,333 - -0.2 997,393 - -2.7 997,458 - Synpcc7942_0989 0.18 +0.8 997,459 - Synpcc7942_0989 0.18 +0.0 997,473 + Synpcc7942_0989 0.21 -1.7 997,507 + Synpcc7942_0989 0.29 +0.4 997,515 - Synpcc7942_0989 0.31 +0.7 997,518 + +1.1 997,526 - Synpcc7942_0989 0.33 +1.0 997,529 + Synpcc7942_0989 0.34 -1.8 997,529 + Synpcc7942_0989 0.34 +1.8 997,531 - Synpcc7942_0989 0.34 +0.4 997,537 - Synpcc7942_0989 0.36 -2.1 997,537 - Synpcc7942_0989 0.36 +2.2 997,561 + Synpcc7942_0989 0.41 +1.0 997,561 + Synpcc7942_0989 0.41 -0.5 997,561 + Synpcc7942_0989 0.41 +0.3 997,561 + Synpcc7942_0989 0.41 +0.6 997,569 - Synpcc7942_0989 0.43 +1.9 997,569 - Synpcc7942_0989 0.43 +0.9 997,569 - Synpcc7942_0989 0.43 +0.2 997,569 - Synpcc7942_0989 0.43 -2.1 997,580 + Synpcc7942_0989 0.45 +0.5 997,582 - Synpcc7942_0989 0.46 +0.6 997,599 + Synpcc7942_0989 0.50 +0.4 997,607 - Synpcc7942_0989 0.51 +0.5 997,607 - Synpcc7942_0989 0.51 +0.7 997,607 - -0.1 997,626 + Synpcc7942_0989 0.56 -2.0 997,652 - Synpcc7942_0989 0.61 +0.8 997,695 + Synpcc7942_0989 0.71 -2.0 997,700 + Synpcc7942_0989 0.72 +0.3 997,703 - Synpcc7942_0989 0.73 -2.8 997,723 + Synpcc7942_0989 0.77 +1.0 997,731 + Synpcc7942_0989 0.79 -2.4 997,731 + Synpcc7942_0989 0.79 +3.4 997,731 + Synpcc7942_0989 0.79 -1.2 997,744 - Synpcc7942_0989 0.82 +0.7 997,766 + Synpcc7942_0989 0.87 +1.4 997,774 + Synpcc7942_0989 0.89 +1.5 997,776 - Synpcc7942_0989 0.89 +0.2 997,820 - +0.8 997,843 + +0.7 998,452 - -0.2 998,642 + pxcA Synpcc7942_0991 0.14 -0.1 998,647 + pxcA Synpcc7942_0991 0.14 +0.5 998,647 + pxcA Synpcc7942_0991 0.14 -1.3 998,653 + pxcA Synpcc7942_0991 0.15 -1.9
Or see this region's nucleotide sequence