Experiment: Flask grown at 18C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_0104 and nadE are separated by 244 nucleotides nadE and nadD overlap by 4 nucleotides nadD and Synpcc7942_0107 are separated by 12 nucleotides
Synpcc7942_0104: Synpcc7942_0104 - hypothetical protein, at 103,632 to 104,408
_0104
Synpcc7942_0105: nadE - NAD synthetase, at 104,653 to 106,377
nadE
Synpcc7942_0106: nadD - nicotinic acid mononucleotide adenyltransferase, at 106,374 to 106,952
nadD
Synpcc7942_0107: Synpcc7942_0107 - Small GTP-binding protein domain, at 106,965 to 108,383
_0107
Position (kb) 104
105
106
107 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 103.700 kb on - strand at 103.757 kb on - strand, within Synpcc7942_0104 at 103.757 kb on - strand, within Synpcc7942_0104 at 103.907 kb on - strand, within Synpcc7942_0104 at 103.947 kb on + strand, within Synpcc7942_0104 at 103.957 kb on + strand, within Synpcc7942_0104 at 103.957 kb on + strand, within Synpcc7942_0104 at 103.957 kb on + strand, within Synpcc7942_0104 at 103.989 kb on - strand, within Synpcc7942_0104 at 103.992 kb on + strand, within Synpcc7942_0104 at 104.047 kb on + strand, within Synpcc7942_0104 at 104.117 kb on + strand, within Synpcc7942_0104 at 104.137 kb on + strand, within Synpcc7942_0104 at 104.148 kb on - strand, within Synpcc7942_0104 at 104.188 kb on + strand, within Synpcc7942_0104 at 104.188 kb on + strand, within Synpcc7942_0104 at 104.196 kb on - strand, within Synpcc7942_0104 at 104.196 kb on - strand, within Synpcc7942_0104 at 104.239 kb on + strand, within Synpcc7942_0104 at 104.247 kb on + strand, within Synpcc7942_0104 at 104.258 kb on - strand, within Synpcc7942_0104 at 104.280 kb on + strand, within Synpcc7942_0104 at 104.287 kb on + strand, within Synpcc7942_0104 at 104.288 kb on - strand, within Synpcc7942_0104 at 104.294 kb on + strand, within Synpcc7942_0104 at 104.299 kb on + strand, within Synpcc7942_0104 at 104.302 kb on - strand, within Synpcc7942_0104 at 104.319 kb on - strand, within Synpcc7942_0104 at 104.331 kb on + strand at 104.339 kb on - strand at 104.346 kb on + strand at 104.364 kb on - strand at 104.365 kb on - strand at 104.380 kb on + strand at 104.574 kb on + strand at 104.581 kb on - strand at 106.379 kb on - strand at 106.396 kb on + strand at 106.396 kb on + strand at 106.396 kb on + strand at 106.404 kb on - strand at 106.410 kb on - strand at 106.763 kb on - strand, within nadD at 106.763 kb on - strand, within nadD at 106.801 kb on - strand, within nadD at 106.829 kb on + strand, within nadD at 106.837 kb on - strand, within nadD at 106.892 kb on - strand, within nadD at 106.935 kb on + strand at 106.954 kb on + strand at 106.955 kb on - strand at 107.004 kb on + strand at 107.030 kb on + strand at 107.038 kb on - strand at 107.040 kb on + strand at 107.079 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown at 18C remove 103,700 - -0.7 103,757 - Synpcc7942_0104 0.16 -2.5 103,757 - Synpcc7942_0104 0.16 -0.4 103,907 - Synpcc7942_0104 0.35 -1.3 103,947 + Synpcc7942_0104 0.41 -1.3 103,957 + Synpcc7942_0104 0.42 +0.7 103,957 + Synpcc7942_0104 0.42 -1.9 103,957 + Synpcc7942_0104 0.42 +1.4 103,989 - Synpcc7942_0104 0.46 +2.0 103,992 + Synpcc7942_0104 0.46 -1.2 104,047 + Synpcc7942_0104 0.53 +1.4 104,117 + Synpcc7942_0104 0.62 +0.1 104,137 + Synpcc7942_0104 0.65 -0.2 104,148 - Synpcc7942_0104 0.66 +0.7 104,188 + Synpcc7942_0104 0.72 -2.1 104,188 + Synpcc7942_0104 0.72 +1.9 104,196 - Synpcc7942_0104 0.73 +0.5 104,196 - Synpcc7942_0104 0.73 +0.5 104,239 + Synpcc7942_0104 0.78 +0.4 104,247 + Synpcc7942_0104 0.79 -0.9 104,258 - Synpcc7942_0104 0.81 +0.1 104,280 + Synpcc7942_0104 0.83 +0.7 104,287 + Synpcc7942_0104 0.84 -1.7 104,288 - Synpcc7942_0104 0.84 -1.4 104,294 + Synpcc7942_0104 0.85 +1.4 104,299 + Synpcc7942_0104 0.86 -2.7 104,302 - Synpcc7942_0104 0.86 +1.4 104,319 - Synpcc7942_0104 0.88 -2.1 104,331 + +1.8 104,339 - +0.8 104,346 + +2.1 104,364 - +0.9 104,365 - +2.0 104,380 + +1.3 104,574 + -1.9 104,581 - +2.0 106,379 - +0.5 106,396 + -4.0 106,396 + -3.1 106,396 + -0.8 106,404 - -1.9 106,410 - +0.5 106,763 - nadD Synpcc7942_0106 0.67 +2.5 106,763 - nadD Synpcc7942_0106 0.67 -0.8 106,801 - nadD Synpcc7942_0106 0.74 -2.0 106,829 + nadD Synpcc7942_0106 0.79 -1.8 106,837 - nadD Synpcc7942_0106 0.80 +0.3 106,892 - nadD Synpcc7942_0106 0.89 -2.5 106,935 + +0.5 106,954 + -0.3 106,955 - +0.4 107,004 + -3.1 107,030 + -4.0 107,038 - -2.7 107,040 + -1.1 107,079 + -3.6
Or see this region's nucleotide sequence