Experiment: Flask grown at 18C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pyk2 and Synpcc7942_0099 are separated by 115 nucleotides Synpcc7942_0099 and Synpcc7942_0100 overlap by 4 nucleotides Synpcc7942_0100 and ndbA are separated by 176 nucleotides
Synpcc7942_0098: pyk2 - pyruvate kinase, at 98,302 to 100,086
pyk2
Synpcc7942_0099: Synpcc7942_0099 - hypothetical protein, at 100,202 to 100,531
_0099
Synpcc7942_0100: Synpcc7942_0100 - hypothetical protein, at 100,528 to 101,082
_0100
Synpcc7942_0101: ndbA - type 2 NADH dehydrogenase, at 101,259 to 102,593
ndbA
Position (kb)
100
101
102 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 100.153 kb on - strand at 100.167 kb on + strand at 100.194 kb on + strand at 100.248 kb on + strand, within Synpcc7942_0099 at 100.250 kb on + strand, within Synpcc7942_0099 at 100.257 kb on - strand, within Synpcc7942_0099 at 100.260 kb on + strand, within Synpcc7942_0099 at 100.281 kb on + strand, within Synpcc7942_0099 at 100.281 kb on + strand, within Synpcc7942_0099 at 100.281 kb on + strand, within Synpcc7942_0099 at 100.281 kb on + strand, within Synpcc7942_0099 at 100.281 kb on + strand, within Synpcc7942_0099 at 100.281 kb on + strand, within Synpcc7942_0099 at 100.288 kb on - strand, within Synpcc7942_0099 at 100.289 kb on - strand, within Synpcc7942_0099 at 100.289 kb on - strand, within Synpcc7942_0099 at 100.289 kb on - strand, within Synpcc7942_0099 at 100.289 kb on - strand, within Synpcc7942_0099 at 100.289 kb on - strand, within Synpcc7942_0099 at 100.289 kb on - strand, within Synpcc7942_0099 at 100.289 kb on - strand, within Synpcc7942_0099 at 100.289 kb on - strand, within Synpcc7942_0099 at 100.289 kb on - strand, within Synpcc7942_0099 at 100.289 kb on - strand, within Synpcc7942_0099 at 100.289 kb on - strand, within Synpcc7942_0099 at 100.289 kb on - strand, within Synpcc7942_0099 at 100.289 kb on - strand, within Synpcc7942_0099 at 100.289 kb on - strand, within Synpcc7942_0099 at 100.349 kb on + strand, within Synpcc7942_0099 at 100.388 kb on + strand, within Synpcc7942_0099 at 100.388 kb on + strand, within Synpcc7942_0099 at 100.402 kb on + strand, within Synpcc7942_0099 at 100.407 kb on + strand, within Synpcc7942_0099 at 100.408 kb on - strand, within Synpcc7942_0099 at 100.410 kb on - strand, within Synpcc7942_0099 at 100.415 kb on - strand, within Synpcc7942_0099 at 100.430 kb on + strand, within Synpcc7942_0099 at 100.443 kb on - strand, within Synpcc7942_0099 at 100.449 kb on - strand, within Synpcc7942_0099 at 100.487 kb on + strand, within Synpcc7942_0099 at 100.534 kb on - strand at 100.539 kb on - strand at 100.596 kb on - strand, within Synpcc7942_0100 at 100.596 kb on - strand, within Synpcc7942_0100 at 100.617 kb on - strand, within Synpcc7942_0100 at 100.617 kb on - strand, within Synpcc7942_0100 at 100.617 kb on - strand, within Synpcc7942_0100 at 100.617 kb on - strand, within Synpcc7942_0100 at 100.627 kb on - strand, within Synpcc7942_0100 at 100.644 kb on - strand, within Synpcc7942_0100 at 100.644 kb on - strand, within Synpcc7942_0100 at 100.652 kb on - strand, within Synpcc7942_0100 at 100.662 kb on - strand, within Synpcc7942_0100 at 100.677 kb on + strand, within Synpcc7942_0100 at 100.689 kb on + strand, within Synpcc7942_0100 at 100.708 kb on + strand, within Synpcc7942_0100 at 100.716 kb on - strand, within Synpcc7942_0100 at 100.727 kb on + strand, within Synpcc7942_0100 at 100.727 kb on + strand, within Synpcc7942_0100 at 100.727 kb on + strand, within Synpcc7942_0100 at 100.735 kb on - strand, within Synpcc7942_0100 at 100.759 kb on + strand, within Synpcc7942_0100 at 100.784 kb on + strand, within Synpcc7942_0100 at 100.784 kb on + strand, within Synpcc7942_0100 at 100.784 kb on + strand, within Synpcc7942_0100 at 100.812 kb on + strand, within Synpcc7942_0100 at 100.815 kb on - strand, within Synpcc7942_0100 at 100.869 kb on + strand, within Synpcc7942_0100 at 100.869 kb on + strand, within Synpcc7942_0100 at 100.872 kb on - strand, within Synpcc7942_0100 at 100.877 kb on - strand, within Synpcc7942_0100 at 100.897 kb on + strand, within Synpcc7942_0100 at 100.897 kb on + strand, within Synpcc7942_0100 at 100.897 kb on + strand, within Synpcc7942_0100 at 100.905 kb on - strand, within Synpcc7942_0100 at 100.905 kb on - strand, within Synpcc7942_0100 at 100.905 kb on - strand, within Synpcc7942_0100 at 100.927 kb on + strand, within Synpcc7942_0100 at 100.927 kb on + strand, within Synpcc7942_0100 at 100.940 kb on - strand, within Synpcc7942_0100 at 100.943 kb on + strand, within Synpcc7942_0100 at 100.943 kb on + strand, within Synpcc7942_0100 at 100.956 kb on - strand, within Synpcc7942_0100 at 101.033 kb on + strand at 101.042 kb on + strand at 101.055 kb on + strand at 101.055 kb on - strand at 101.100 kb on + strand at 101.100 kb on + strand at 101.108 kb on - strand at 101.108 kb on - strand at 101.108 kb on - strand at 101.111 kb on + strand at 101.111 kb on + strand at 101.111 kb on + strand at 101.111 kb on + strand at 101.149 kb on + strand at 101.149 kb on + strand at 101.156 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Flask grown at 18C remove 100,153 - +2.0 100,167 + +2.5 100,194 + -0.5 100,248 + Synpcc7942_0099 0.14 +1.9 100,250 + Synpcc7942_0099 0.15 +3.3 100,257 - Synpcc7942_0099 0.17 +0.8 100,260 + Synpcc7942_0099 0.18 -1.3 100,281 + Synpcc7942_0099 0.24 +1.0 100,281 + Synpcc7942_0099 0.24 -1.2 100,281 + Synpcc7942_0099 0.24 -1.3 100,281 + Synpcc7942_0099 0.24 +2.2 100,281 + Synpcc7942_0099 0.24 -1.2 100,281 + Synpcc7942_0099 0.24 +2.8 100,288 - Synpcc7942_0099 0.26 +1.7 100,289 - Synpcc7942_0099 0.26 +0.9 100,289 - Synpcc7942_0099 0.26 -0.5 100,289 - Synpcc7942_0099 0.26 +0.1 100,289 - Synpcc7942_0099 0.26 +0.8 100,289 - Synpcc7942_0099 0.26 +0.6 100,289 - Synpcc7942_0099 0.26 -0.9 100,289 - Synpcc7942_0099 0.26 -0.1 100,289 - Synpcc7942_0099 0.26 -0.5 100,289 - Synpcc7942_0099 0.26 +2.0 100,289 - Synpcc7942_0099 0.26 +1.3 100,289 - Synpcc7942_0099 0.26 +0.9 100,289 - Synpcc7942_0099 0.26 +0.6 100,289 - Synpcc7942_0099 0.26 +1.8 100,289 - Synpcc7942_0099 0.26 -0.7 100,349 + Synpcc7942_0099 0.45 +0.7 100,388 + Synpcc7942_0099 0.56 +0.1 100,388 + Synpcc7942_0099 0.56 -4.2 100,402 + Synpcc7942_0099 0.61 -3.0 100,407 + Synpcc7942_0099 0.62 +0.5 100,408 - Synpcc7942_0099 0.62 -0.2 100,410 - Synpcc7942_0099 0.63 +0.5 100,415 - Synpcc7942_0099 0.65 -0.6 100,430 + Synpcc7942_0099 0.69 +1.6 100,443 - Synpcc7942_0099 0.73 +1.2 100,449 - Synpcc7942_0099 0.75 +2.4 100,487 + Synpcc7942_0099 0.86 -1.1 100,534 - +0.3 100,539 - +1.5 100,596 - Synpcc7942_0100 0.12 +2.6 100,596 - Synpcc7942_0100 0.12 +0.8 100,617 - Synpcc7942_0100 0.16 +0.5 100,617 - Synpcc7942_0100 0.16 +0.2 100,617 - Synpcc7942_0100 0.16 +2.2 100,617 - Synpcc7942_0100 0.16 +1.3 100,627 - Synpcc7942_0100 0.18 -0.2 100,644 - Synpcc7942_0100 0.21 +1.7 100,644 - Synpcc7942_0100 0.21 +2.4 100,652 - Synpcc7942_0100 0.22 +1.8 100,662 - Synpcc7942_0100 0.24 -3.2 100,677 + Synpcc7942_0100 0.27 -4.0 100,689 + Synpcc7942_0100 0.29 +0.3 100,708 + Synpcc7942_0100 0.32 +2.3 100,716 - Synpcc7942_0100 0.34 +1.7 100,727 + Synpcc7942_0100 0.36 +1.2 100,727 + Synpcc7942_0100 0.36 -0.1 100,727 + Synpcc7942_0100 0.36 +2.3 100,735 - Synpcc7942_0100 0.37 +0.6 100,759 + Synpcc7942_0100 0.42 -0.7 100,784 + Synpcc7942_0100 0.46 +0.7 100,784 + Synpcc7942_0100 0.46 -1.5 100,784 + Synpcc7942_0100 0.46 +2.1 100,812 + Synpcc7942_0100 0.51 +1.1 100,815 - Synpcc7942_0100 0.52 -2.0 100,869 + Synpcc7942_0100 0.61 +0.2 100,869 + Synpcc7942_0100 0.61 +2.5 100,872 - Synpcc7942_0100 0.62 +1.5 100,877 - Synpcc7942_0100 0.63 +2.2 100,897 + Synpcc7942_0100 0.66 -0.1 100,897 + Synpcc7942_0100 0.66 +1.7 100,897 + Synpcc7942_0100 0.66 +0.9 100,905 - Synpcc7942_0100 0.68 +1.1 100,905 - Synpcc7942_0100 0.68 -5.0 100,905 - Synpcc7942_0100 0.68 -2.5 100,927 + Synpcc7942_0100 0.72 +1.9 100,927 + Synpcc7942_0100 0.72 +2.0 100,940 - Synpcc7942_0100 0.74 +2.3 100,943 + Synpcc7942_0100 0.75 +1.8 100,943 + Synpcc7942_0100 0.75 -0.1 100,956 - Synpcc7942_0100 0.77 +1.3 101,033 + +2.1 101,042 + -1.8 101,055 + -0.8 101,055 - +1.1 101,100 + +0.9 101,100 + -2.2 101,108 - +0.5 101,108 - +1.3 101,108 - -2.8 101,111 + +0.0 101,111 + +2.0 101,111 + +1.6 101,111 + -1.4 101,149 + -2.7 101,149 + -1.7 101,156 + -1.6
Or see this region's nucleotide sequence