Experiment: Carnitine Hydrochloride (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_501 and Pf6N2E2_502 are separated by 239 nucleotides Pf6N2E2_502 and Pf6N2E2_503 are separated by 185 nucleotides Pf6N2E2_503 and Pf6N2E2_504 overlap by 23 nucleotides Pf6N2E2_504 and Pf6N2E2_505 are separated by 24 nucleotides Pf6N2E2_505 and Pf6N2E2_506 overlap by 35 nucleotides
Pf6N2E2_501 - Sensory box transcriptional regulator, LuxR family, at 570,546 to 572,030
_501
Pf6N2E2_502 - hypothetical protein, at 572,270 to 572,515
_502
Pf6N2E2_503 - sensor histidine kinase, at 572,701 to 574,086
_503
Pf6N2E2_504 - DNA-binding response regulator, at 574,064 to 574,747
_504
Pf6N2E2_505 - hypothetical protein, at 574,772 to 575,053
_505
Pf6N2E2_506 - Hydrogen cyanide synthase HcnA / Opine oxidase subunit C, at 575,019 to 575,342
_506
Position (kb) 572
573
574
575 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 571.908 kb on - strand at 571.929 kb on - strand at 571.935 kb on + strand at 571.996 kb on + strand at 572.177 kb on + strand at 572.178 kb on - strand at 572.185 kb on + strand at 572.186 kb on - strand at 572.186 kb on - strand at 572.205 kb on + strand at 572.205 kb on + strand at 572.205 kb on + strand at 572.206 kb on - strand at 572.364 kb on + strand, within Pf6N2E2_502 at 572.372 kb on + strand, within Pf6N2E2_502 at 572.373 kb on - strand, within Pf6N2E2_502 at 572.373 kb on - strand, within Pf6N2E2_502 at 572.474 kb on + strand, within Pf6N2E2_502 at 572.489 kb on - strand, within Pf6N2E2_502 at 572.561 kb on - strand at 572.561 kb on - strand at 572.561 kb on - strand at 572.637 kb on + strand at 572.703 kb on - strand at 572.761 kb on + strand at 572.761 kb on + strand at 572.761 kb on + strand at 572.855 kb on + strand, within Pf6N2E2_503 at 572.856 kb on - strand, within Pf6N2E2_503 at 573.025 kb on + strand, within Pf6N2E2_503 at 573.025 kb on + strand, within Pf6N2E2_503 at 573.025 kb on + strand, within Pf6N2E2_503 at 573.026 kb on - strand, within Pf6N2E2_503 at 573.110 kb on + strand, within Pf6N2E2_503 at 573.110 kb on + strand, within Pf6N2E2_503 at 573.133 kb on + strand, within Pf6N2E2_503 at 573.652 kb on + strand, within Pf6N2E2_503 at 573.967 kb on + strand at 573.968 kb on - strand at 573.968 kb on - strand at 573.974 kb on + strand at 573.974 kb on + strand at 573.974 kb on + strand at 573.975 kb on - strand at 573.975 kb on - strand at 573.977 kb on + strand at 573.977 kb on + strand at 573.977 kb on + strand at 573.977 kb on + strand at 573.977 kb on + strand at 573.978 kb on - strand at 573.978 kb on - strand at 573.978 kb on - strand at 573.978 kb on - strand at 573.978 kb on - strand at 574.025 kb on + strand at 574.026 kb on - strand at 574.026 kb on - strand at 574.026 kb on - strand at 574.197 kb on + strand, within Pf6N2E2_504 at 574.276 kb on - strand, within Pf6N2E2_504 at 574.361 kb on + strand, within Pf6N2E2_504 at 574.612 kb on - strand, within Pf6N2E2_504 at 574.776 kb on + strand at 574.776 kb on + strand at 574.802 kb on - strand, within Pf6N2E2_505 at 574.878 kb on + strand, within Pf6N2E2_505 at 574.878 kb on + strand, within Pf6N2E2_505 at 574.878 kb on + strand, within Pf6N2E2_505 at 574.890 kb on + strand, within Pf6N2E2_505 at 574.932 kb on + strand, within Pf6N2E2_505 at 574.933 kb on - strand, within Pf6N2E2_505 at 575.015 kb on + strand, within Pf6N2E2_505 at 575.015 kb on + strand, within Pf6N2E2_505 at 575.015 kb on + strand, within Pf6N2E2_505 at 575.015 kb on + strand, within Pf6N2E2_505 at 575.016 kb on - strand, within Pf6N2E2_505 at 575.016 kb on - strand, within Pf6N2E2_505 at 575.016 kb on - strand, within Pf6N2E2_505 at 575.016 kb on - strand, within Pf6N2E2_505 at 575.038 kb on + strand at 575.038 kb on + strand at 575.039 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carnitine Hydrochloride (C) remove 571,908 - -1.9 571,929 - +1.3 571,935 + +0.1 571,996 + -1.1 572,177 + -0.9 572,178 - +0.1 572,185 + -0.0 572,186 - +1.1 572,186 - -0.5 572,205 + +1.1 572,205 + +0.3 572,205 + +1.4 572,206 - +0.7 572,364 + Pf6N2E2_502 0.38 +0.9 572,372 + Pf6N2E2_502 0.41 -0.6 572,373 - Pf6N2E2_502 0.42 +0.8 572,373 - Pf6N2E2_502 0.42 +0.9 572,474 + Pf6N2E2_502 0.83 -0.7 572,489 - Pf6N2E2_502 0.89 -0.4 572,561 - +0.5 572,561 - +0.9 572,561 - +0.2 572,637 + +1.1 572,703 - +0.6 572,761 + -3.6 572,761 + -3.8 572,761 + -2.2 572,855 + Pf6N2E2_503 0.11 -1.5 572,856 - Pf6N2E2_503 0.11 -0.6 573,025 + Pf6N2E2_503 0.23 +0.2 573,025 + Pf6N2E2_503 0.23 -1.7 573,025 + Pf6N2E2_503 0.23 -0.4 573,026 - Pf6N2E2_503 0.23 -1.0 573,110 + Pf6N2E2_503 0.30 -2.6 573,110 + Pf6N2E2_503 0.30 -1.9 573,133 + Pf6N2E2_503 0.31 -2.5 573,652 + Pf6N2E2_503 0.69 -0.2 573,967 + -2.4 573,968 - -2.2 573,968 - -1.2 573,974 + -1.0 573,974 + -0.2 573,974 + -1.1 573,975 - -0.7 573,975 - -1.0 573,977 + -2.9 573,977 + -1.5 573,977 + -1.5 573,977 + -1.4 573,977 + -0.9 573,978 - -2.5 573,978 - -0.8 573,978 - -2.0 573,978 - -2.9 573,978 - -1.4 574,025 + -4.6 574,026 - -1.1 574,026 - -0.8 574,026 - -0.3 574,197 + Pf6N2E2_504 0.19 -2.7 574,276 - Pf6N2E2_504 0.31 -2.3 574,361 + Pf6N2E2_504 0.43 -3.6 574,612 - Pf6N2E2_504 0.80 -0.8 574,776 + -1.9 574,776 + -1.1 574,802 - Pf6N2E2_505 0.11 +1.9 574,878 + Pf6N2E2_505 0.38 -0.3 574,878 + Pf6N2E2_505 0.38 +0.6 574,878 + Pf6N2E2_505 0.38 -1.3 574,890 + Pf6N2E2_505 0.42 +0.1 574,932 + Pf6N2E2_505 0.57 +3.0 574,933 - Pf6N2E2_505 0.57 -0.4 575,015 + Pf6N2E2_505 0.86 +0.7 575,015 + Pf6N2E2_505 0.86 +0.2 575,015 + Pf6N2E2_505 0.86 +0.4 575,015 + Pf6N2E2_505 0.86 +0.3 575,016 - Pf6N2E2_505 0.87 +0.4 575,016 - Pf6N2E2_505 0.87 -0.5 575,016 - Pf6N2E2_505 0.87 +0.5 575,016 - Pf6N2E2_505 0.87 -0.1 575,038 + +0.5 575,038 + -0.3 575,039 - +0.4
Or see this region's nucleotide sequence