Experiment: Carnitine Hydrochloride (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4715 and Pf6N2E2_4716 are separated by 195 nucleotides Pf6N2E2_4716 and Pf6N2E2_4717 are separated by 308 nucleotides
Pf6N2E2_4715 - Low-specificity L-threonine aldolase (EC 4.1.2.48), at 5,451,671 to 5,452,711
_4715
Pf6N2E2_4716 - Membrane protein, putative, at 5,452,907 to 5,453,611
_4716
Pf6N2E2_4717 - Serine hydroxymethyltransferase (EC 2.1.2.1), at 5,453,920 to 5,455,173
_4717
Position (kb)
5452
5453
5454 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 5452.027 kb on - strand, within Pf6N2E2_4715 at 5452.258 kb on - strand, within Pf6N2E2_4715 at 5452.258 kb on - strand, within Pf6N2E2_4715 at 5452.476 kb on + strand, within Pf6N2E2_4715 at 5452.608 kb on + strand at 5452.674 kb on + strand at 5452.674 kb on + strand at 5452.729 kb on - strand at 5452.729 kb on - strand at 5452.729 kb on - strand at 5452.729 kb on - strand at 5452.902 kb on + strand at 5452.902 kb on + strand at 5452.902 kb on + strand at 5452.902 kb on + strand at 5452.903 kb on - strand at 5452.903 kb on - strand at 5452.903 kb on - strand at 5452.983 kb on - strand, within Pf6N2E2_4716 at 5453.009 kb on + strand, within Pf6N2E2_4716 at 5453.009 kb on + strand, within Pf6N2E2_4716 at 5453.009 kb on + strand, within Pf6N2E2_4716 at 5453.009 kb on + strand, within Pf6N2E2_4716 at 5453.009 kb on + strand, within Pf6N2E2_4716 at 5453.010 kb on - strand, within Pf6N2E2_4716 at 5453.010 kb on - strand, within Pf6N2E2_4716 at 5453.010 kb on - strand, within Pf6N2E2_4716 at 5453.010 kb on - strand, within Pf6N2E2_4716 at 5453.010 kb on - strand, within Pf6N2E2_4716 at 5453.010 kb on - strand, within Pf6N2E2_4716 at 5453.010 kb on - strand, within Pf6N2E2_4716 at 5453.010 kb on - strand, within Pf6N2E2_4716 at 5453.119 kb on - strand, within Pf6N2E2_4716 at 5453.119 kb on - strand, within Pf6N2E2_4716 at 5453.145 kb on - strand, within Pf6N2E2_4716 at 5453.272 kb on - strand, within Pf6N2E2_4716 at 5453.272 kb on - strand, within Pf6N2E2_4716 at 5453.364 kb on - strand, within Pf6N2E2_4716 at 5453.364 kb on - strand, within Pf6N2E2_4716 at 5453.420 kb on + strand, within Pf6N2E2_4716 at 5453.420 kb on + strand, within Pf6N2E2_4716 at 5453.420 kb on + strand, within Pf6N2E2_4716 at 5453.421 kb on - strand, within Pf6N2E2_4716 at 5453.552 kb on + strand at 5453.673 kb on - strand at 5453.784 kb on + strand at 5453.837 kb on + strand at 5453.838 kb on - strand at 5453.838 kb on - strand at 5453.838 kb on - strand at 5453.951 kb on - strand at 5454.032 kb on - strand at 5454.082 kb on + strand, within Pf6N2E2_4717 at 5454.112 kb on + strand, within Pf6N2E2_4717 at 5454.112 kb on + strand, within Pf6N2E2_4717 at 5454.113 kb on - strand, within Pf6N2E2_4717 at 5454.233 kb on + strand, within Pf6N2E2_4717 at 5454.233 kb on + strand, within Pf6N2E2_4717 at 5454.234 kb on - strand, within Pf6N2E2_4717 at 5454.234 kb on - strand, within Pf6N2E2_4717 at 5454.236 kb on - strand, within Pf6N2E2_4717 at 5454.337 kb on + strand, within Pf6N2E2_4717 at 5454.337 kb on + strand, within Pf6N2E2_4717 at 5454.338 kb on - strand, within Pf6N2E2_4717 at 5454.352 kb on + strand, within Pf6N2E2_4717 at 5454.385 kb on + strand, within Pf6N2E2_4717 at 5454.452 kb on - strand, within Pf6N2E2_4717
Per-strain Table
Position Strand Gene LocusTag Fraction Carnitine Hydrochloride (C) remove 5,452,027 - Pf6N2E2_4715 0.34 +0.4 5,452,258 - Pf6N2E2_4715 0.56 -1.2 5,452,258 - Pf6N2E2_4715 0.56 +1.0 5,452,476 + Pf6N2E2_4715 0.77 +1.3 5,452,608 + -0.4 5,452,674 + +0.1 5,452,674 + +0.4 5,452,729 - +0.1 5,452,729 - -0.1 5,452,729 - +0.1 5,452,729 - +0.2 5,452,902 + -0.2 5,452,902 + +0.6 5,452,902 + +0.1 5,452,902 + +0.2 5,452,903 - +0.4 5,452,903 - -0.1 5,452,903 - +1.4 5,452,983 - Pf6N2E2_4716 0.11 -0.5 5,453,009 + Pf6N2E2_4716 0.14 +0.0 5,453,009 + Pf6N2E2_4716 0.14 +0.4 5,453,009 + Pf6N2E2_4716 0.14 +0.7 5,453,009 + Pf6N2E2_4716 0.14 +0.7 5,453,009 + Pf6N2E2_4716 0.14 +0.2 5,453,010 - Pf6N2E2_4716 0.15 +0.4 5,453,010 - Pf6N2E2_4716 0.15 +0.4 5,453,010 - Pf6N2E2_4716 0.15 -0.3 5,453,010 - Pf6N2E2_4716 0.15 +1.1 5,453,010 - Pf6N2E2_4716 0.15 +1.2 5,453,010 - Pf6N2E2_4716 0.15 +0.1 5,453,010 - Pf6N2E2_4716 0.15 +0.2 5,453,010 - Pf6N2E2_4716 0.15 -2.1 5,453,119 - Pf6N2E2_4716 0.30 -0.1 5,453,119 - Pf6N2E2_4716 0.30 +0.8 5,453,145 - Pf6N2E2_4716 0.34 -0.7 5,453,272 - Pf6N2E2_4716 0.52 +0.9 5,453,272 - Pf6N2E2_4716 0.52 +0.1 5,453,364 - Pf6N2E2_4716 0.65 -0.4 5,453,364 - Pf6N2E2_4716 0.65 -0.0 5,453,420 + Pf6N2E2_4716 0.73 +0.4 5,453,420 + Pf6N2E2_4716 0.73 -0.1 5,453,420 + Pf6N2E2_4716 0.73 +1.3 5,453,421 - Pf6N2E2_4716 0.73 +0.1 5,453,552 + -0.1 5,453,673 - -0.1 5,453,784 + -4.6 5,453,837 + -3.2 5,453,838 - -4.5 5,453,838 - -2.9 5,453,838 - -1.5 5,453,951 - -1.6 5,454,032 - -4.6 5,454,082 + Pf6N2E2_4717 0.13 -3.2 5,454,112 + Pf6N2E2_4717 0.15 -4.1 5,454,112 + Pf6N2E2_4717 0.15 -3.2 5,454,113 - Pf6N2E2_4717 0.15 -3.7 5,454,233 + Pf6N2E2_4717 0.25 -3.4 5,454,233 + Pf6N2E2_4717 0.25 -3.5 5,454,234 - Pf6N2E2_4717 0.25 -3.7 5,454,234 - Pf6N2E2_4717 0.25 -3.1 5,454,236 - Pf6N2E2_4717 0.25 -2.8 5,454,337 + Pf6N2E2_4717 0.33 -2.3 5,454,337 + Pf6N2E2_4717 0.33 -1.2 5,454,338 - Pf6N2E2_4717 0.33 -4.1 5,454,352 + Pf6N2E2_4717 0.34 -1.7 5,454,385 + Pf6N2E2_4717 0.37 -3.6 5,454,452 - Pf6N2E2_4717 0.42 -1.3
Or see this region's nucleotide sequence