Experiment: Carnitine Hydrochloride (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4691 and Pf6N2E2_4692 are separated by 38 nucleotides Pf6N2E2_4692 and Pf6N2E2_4693 are separated by 71 nucleotides Pf6N2E2_4693 and Pf6N2E2_4694 are separated by 186 nucleotides
Pf6N2E2_4691 - hypothetical protein, at 5,424,942 to 5,425,070
_4691
Pf6N2E2_4692 - dehydrocarnitine cleavage enzyme (EC 2.3.1.-) (from data) , at 5,425,109 to 5,425,996
_4692
Pf6N2E2_4693 - ABC transporter for Carnitine Hydrochloride, periplasmic substrate-binding component (from data) , at 5,426,068 to 5,427,012
_4693
Pf6N2E2_4694 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 5,427,199 to 5,428,143
_4694
Position (kb)
5426
5427
5428 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 5425.099 kb on + strand at 5425.099 kb on + strand at 5425.100 kb on - strand at 5425.100 kb on - strand at 5425.100 kb on - strand at 5425.100 kb on - strand at 5425.226 kb on + strand, within Pf6N2E2_4692 at 5425.226 kb on + strand, within Pf6N2E2_4692 at 5425.226 kb on + strand, within Pf6N2E2_4692 at 5425.227 kb on - strand, within Pf6N2E2_4692 at 5425.227 kb on - strand, within Pf6N2E2_4692 at 5425.572 kb on + strand, within Pf6N2E2_4692 at 5425.572 kb on + strand, within Pf6N2E2_4692 at 5425.572 kb on + strand, within Pf6N2E2_4692 at 5425.572 kb on + strand, within Pf6N2E2_4692 at 5425.572 kb on + strand, within Pf6N2E2_4692 at 5425.572 kb on + strand, within Pf6N2E2_4692 at 5425.572 kb on + strand, within Pf6N2E2_4692 at 5425.572 kb on + strand, within Pf6N2E2_4692 at 5425.572 kb on + strand, within Pf6N2E2_4692 at 5425.573 kb on - strand, within Pf6N2E2_4692 at 5425.573 kb on - strand, within Pf6N2E2_4692 at 5425.573 kb on - strand, within Pf6N2E2_4692 at 5425.573 kb on - strand, within Pf6N2E2_4692 at 5425.573 kb on - strand, within Pf6N2E2_4692 at 5425.573 kb on - strand, within Pf6N2E2_4692 at 5425.573 kb on - strand, within Pf6N2E2_4692 at 5425.804 kb on - strand, within Pf6N2E2_4692 at 5426.020 kb on + strand at 5426.020 kb on + strand at 5426.020 kb on + strand at 5426.021 kb on - strand at 5426.073 kb on + strand at 5426.118 kb on + strand at 5426.118 kb on + strand at 5426.118 kb on + strand at 5426.118 kb on + strand at 5426.119 kb on - strand at 5426.119 kb on - strand at 5426.366 kb on + strand, within Pf6N2E2_4693 at 5426.366 kb on + strand, within Pf6N2E2_4693 at 5426.367 kb on - strand, within Pf6N2E2_4693 at 5426.570 kb on + strand, within Pf6N2E2_4693 at 5426.570 kb on + strand, within Pf6N2E2_4693 at 5426.570 kb on + strand, within Pf6N2E2_4693 at 5426.570 kb on + strand, within Pf6N2E2_4693 at 5426.570 kb on + strand, within Pf6N2E2_4693 at 5426.571 kb on - strand, within Pf6N2E2_4693 at 5426.571 kb on - strand, within Pf6N2E2_4693 at 5426.642 kb on + strand, within Pf6N2E2_4693 at 5426.642 kb on + strand, within Pf6N2E2_4693 at 5426.642 kb on + strand, within Pf6N2E2_4693 at 5426.643 kb on - strand, within Pf6N2E2_4693 at 5426.837 kb on + strand, within Pf6N2E2_4693 at 5426.986 kb on + strand at 5426.986 kb on + strand at 5426.987 kb on - strand at 5426.987 kb on - strand at 5426.987 kb on - strand at 5426.987 kb on - strand at 5427.032 kb on + strand at 5427.032 kb on + strand at 5427.032 kb on + strand at 5427.032 kb on + strand at 5427.033 kb on - strand at 5427.033 kb on - strand at 5427.076 kb on - strand at 5427.160 kb on - strand at 5427.657 kb on + strand, within Pf6N2E2_4694 at 5427.657 kb on + strand, within Pf6N2E2_4694 at 5427.658 kb on - strand, within Pf6N2E2_4694 at 5427.812 kb on - strand, within Pf6N2E2_4694 at 5427.973 kb on + strand, within Pf6N2E2_4694 at 5427.973 kb on + strand, within Pf6N2E2_4694 at 5427.974 kb on - strand, within Pf6N2E2_4694
Per-strain Table
Position Strand Gene LocusTag Fraction Carnitine Hydrochloride (C) remove 5,425,099 + -4.0 5,425,099 + -3.4 5,425,100 - -0.4 5,425,100 - -0.2 5,425,100 - +0.9 5,425,100 - -0.5 5,425,226 + Pf6N2E2_4692 0.13 -3.3 5,425,226 + Pf6N2E2_4692 0.13 -3.8 5,425,226 + Pf6N2E2_4692 0.13 -4.5 5,425,227 - Pf6N2E2_4692 0.13 -3.2 5,425,227 - Pf6N2E2_4692 0.13 -3.0 5,425,572 + Pf6N2E2_4692 0.52 -5.4 5,425,572 + Pf6N2E2_4692 0.52 -3.0 5,425,572 + Pf6N2E2_4692 0.52 -3.8 5,425,572 + Pf6N2E2_4692 0.52 -2.8 5,425,572 + Pf6N2E2_4692 0.52 -1.0 5,425,572 + Pf6N2E2_4692 0.52 -4.5 5,425,572 + Pf6N2E2_4692 0.52 -4.7 5,425,572 + Pf6N2E2_4692 0.52 -1.5 5,425,572 + Pf6N2E2_4692 0.52 -3.2 5,425,573 - Pf6N2E2_4692 0.52 -3.1 5,425,573 - Pf6N2E2_4692 0.52 -2.6 5,425,573 - Pf6N2E2_4692 0.52 -4.5 5,425,573 - Pf6N2E2_4692 0.52 -3.5 5,425,573 - Pf6N2E2_4692 0.52 -2.8 5,425,573 - Pf6N2E2_4692 0.52 -4.6 5,425,573 - Pf6N2E2_4692 0.52 -3.2 5,425,804 - Pf6N2E2_4692 0.78 -1.8 5,426,020 + -3.3 5,426,020 + -2.5 5,426,020 + -1.8 5,426,021 - -1.0 5,426,073 + -2.3 5,426,118 + -4.1 5,426,118 + -2.9 5,426,118 + +0.1 5,426,118 + -3.8 5,426,119 - -3.8 5,426,119 - -3.7 5,426,366 + Pf6N2E2_4693 0.32 -3.6 5,426,366 + Pf6N2E2_4693 0.32 -1.5 5,426,367 - Pf6N2E2_4693 0.32 -4.1 5,426,570 + Pf6N2E2_4693 0.53 -3.3 5,426,570 + Pf6N2E2_4693 0.53 -4.0 5,426,570 + Pf6N2E2_4693 0.53 -3.4 5,426,570 + Pf6N2E2_4693 0.53 -2.4 5,426,570 + Pf6N2E2_4693 0.53 -1.5 5,426,571 - Pf6N2E2_4693 0.53 -0.0 5,426,571 - Pf6N2E2_4693 0.53 -3.1 5,426,642 + Pf6N2E2_4693 0.61 -0.2 5,426,642 + Pf6N2E2_4693 0.61 -3.8 5,426,642 + Pf6N2E2_4693 0.61 -3.4 5,426,643 - Pf6N2E2_4693 0.61 -4.4 5,426,837 + Pf6N2E2_4693 0.81 -3.5 5,426,986 + -1.7 5,426,986 + -1.4 5,426,987 - -2.6 5,426,987 - -5.0 5,426,987 - -3.8 5,426,987 - -0.9 5,427,032 + -4.4 5,427,032 + -3.8 5,427,032 + -0.6 5,427,032 + -2.6 5,427,033 - -4.0 5,427,033 - -3.8 5,427,076 - -3.3 5,427,160 - -3.4 5,427,657 + Pf6N2E2_4694 0.48 -3.1 5,427,657 + Pf6N2E2_4694 0.48 -4.1 5,427,658 - Pf6N2E2_4694 0.49 -3.7 5,427,812 - Pf6N2E2_4694 0.65 -1.8 5,427,973 + Pf6N2E2_4694 0.82 -4.6 5,427,973 + Pf6N2E2_4694 0.82 -3.9 5,427,974 - Pf6N2E2_4694 0.82 +0.5
Or see this region's nucleotide sequence