Experiment: Carnitine Hydrochloride (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4688 and Pf6N2E2_4689 are separated by 20 nucleotides Pf6N2E2_4689 and Pf6N2E2_4690 are separated by 177 nucleotides
Pf6N2E2_4688 - Gamma-butyrobetaine,2-oxoglutarate dioxygenase (EC 1.14.11.1), at 5,422,126 to 5,423,289
_4688
Pf6N2E2_4689 - L-carnitine 3-dehydrogenase, B subunit (EC 1.1.1.108) (from data) , at 5,423,310 to 5,423,786
_4689
Pf6N2E2_4690 - L-carnitine 3-dehydrogenase, A subunit (EC 1.1.1.108) (from data) , at 5,423,964 to 5,424,929
_4690
Position (kb)
5423
5424 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 5422.627 kb on + strand, within Pf6N2E2_4688 at 5422.627 kb on + strand, within Pf6N2E2_4688 at 5422.677 kb on - strand, within Pf6N2E2_4688 at 5422.964 kb on + strand, within Pf6N2E2_4688 at 5423.051 kb on + strand, within Pf6N2E2_4688 at 5423.051 kb on + strand, within Pf6N2E2_4688 at 5423.052 kb on - strand, within Pf6N2E2_4688 at 5423.052 kb on - strand, within Pf6N2E2_4688 at 5423.052 kb on - strand, within Pf6N2E2_4688 at 5423.347 kb on + strand at 5423.348 kb on - strand at 5423.454 kb on + strand, within Pf6N2E2_4689 at 5423.454 kb on + strand, within Pf6N2E2_4689 at 5423.455 kb on - strand, within Pf6N2E2_4689 at 5423.483 kb on + strand, within Pf6N2E2_4689 at 5423.513 kb on - strand, within Pf6N2E2_4689 at 5423.513 kb on - strand, within Pf6N2E2_4689 at 5423.513 kb on - strand, within Pf6N2E2_4689 at 5423.593 kb on + strand, within Pf6N2E2_4689 at 5423.593 kb on + strand, within Pf6N2E2_4689 at 5423.593 kb on + strand, within Pf6N2E2_4689 at 5423.689 kb on + strand, within Pf6N2E2_4689 at 5423.689 kb on + strand, within Pf6N2E2_4689 at 5423.707 kb on + strand, within Pf6N2E2_4689 at 5423.707 kb on + strand, within Pf6N2E2_4689 at 5423.707 kb on + strand, within Pf6N2E2_4689 at 5423.707 kb on + strand, within Pf6N2E2_4689 at 5423.734 kb on + strand, within Pf6N2E2_4689 at 5423.735 kb on - strand, within Pf6N2E2_4689 at 5423.735 kb on - strand, within Pf6N2E2_4689 at 5423.735 kb on - strand, within Pf6N2E2_4689 at 5423.767 kb on + strand at 5423.767 kb on + strand at 5423.767 kb on + strand at 5423.768 kb on - strand at 5423.768 kb on - strand at 5423.768 kb on - strand at 5423.804 kb on - strand at 5423.804 kb on - strand at 5423.804 kb on - strand at 5423.815 kb on + strand at 5423.816 kb on - strand at 5423.866 kb on - strand at 5423.878 kb on + strand at 5423.878 kb on + strand at 5423.878 kb on + strand at 5423.892 kb on + strand at 5423.893 kb on - strand at 5423.893 kb on - strand at 5423.893 kb on - strand at 5423.893 kb on - strand at 5424.066 kb on - strand, within Pf6N2E2_4690 at 5424.066 kb on - strand, within Pf6N2E2_4690 at 5424.066 kb on - strand, within Pf6N2E2_4690 at 5424.142 kb on + strand, within Pf6N2E2_4690 at 5424.142 kb on + strand, within Pf6N2E2_4690 at 5424.142 kb on + strand, within Pf6N2E2_4690 at 5424.143 kb on - strand, within Pf6N2E2_4690 at 5424.143 kb on - strand, within Pf6N2E2_4690 at 5424.220 kb on + strand, within Pf6N2E2_4690 at 5424.220 kb on + strand, within Pf6N2E2_4690 at 5424.220 kb on + strand, within Pf6N2E2_4690 at 5424.221 kb on - strand, within Pf6N2E2_4690 at 5424.221 kb on - strand, within Pf6N2E2_4690 at 5424.221 kb on - strand, within Pf6N2E2_4690 at 5424.415 kb on + strand, within Pf6N2E2_4690 at 5424.416 kb on - strand, within Pf6N2E2_4690 at 5424.416 kb on - strand, within Pf6N2E2_4690 at 5424.416 kb on - strand, within Pf6N2E2_4690 at 5424.490 kb on + strand, within Pf6N2E2_4690 at 5424.491 kb on - strand, within Pf6N2E2_4690 at 5424.547 kb on + strand, within Pf6N2E2_4690 at 5424.548 kb on - strand, within Pf6N2E2_4690 at 5424.575 kb on + strand, within Pf6N2E2_4690 at 5424.575 kb on + strand, within Pf6N2E2_4690
Per-strain Table
Position Strand Gene LocusTag Fraction Carnitine Hydrochloride (C) remove 5,422,627 + Pf6N2E2_4688 0.43 +1.4 5,422,627 + Pf6N2E2_4688 0.43 +1.0 5,422,677 - Pf6N2E2_4688 0.47 +2.1 5,422,964 + Pf6N2E2_4688 0.72 +1.7 5,423,051 + Pf6N2E2_4688 0.79 +1.1 5,423,051 + Pf6N2E2_4688 0.79 +1.4 5,423,052 - Pf6N2E2_4688 0.80 +1.2 5,423,052 - Pf6N2E2_4688 0.80 +2.2 5,423,052 - Pf6N2E2_4688 0.80 +1.9 5,423,347 + -1.8 5,423,348 - -0.6 5,423,454 + Pf6N2E2_4689 0.30 -1.2 5,423,454 + Pf6N2E2_4689 0.30 -3.6 5,423,455 - Pf6N2E2_4689 0.30 -3.4 5,423,483 + Pf6N2E2_4689 0.36 -3.7 5,423,513 - Pf6N2E2_4689 0.43 -3.4 5,423,513 - Pf6N2E2_4689 0.43 -4.3 5,423,513 - Pf6N2E2_4689 0.43 -2.5 5,423,593 + Pf6N2E2_4689 0.59 -3.0 5,423,593 + Pf6N2E2_4689 0.59 -4.6 5,423,593 + Pf6N2E2_4689 0.59 -3.7 5,423,689 + Pf6N2E2_4689 0.79 -4.5 5,423,689 + Pf6N2E2_4689 0.79 -3.1 5,423,707 + Pf6N2E2_4689 0.83 -3.3 5,423,707 + Pf6N2E2_4689 0.83 -3.8 5,423,707 + Pf6N2E2_4689 0.83 -4.6 5,423,707 + Pf6N2E2_4689 0.83 -4.5 5,423,734 + Pf6N2E2_4689 0.89 -4.0 5,423,735 - Pf6N2E2_4689 0.89 -3.4 5,423,735 - Pf6N2E2_4689 0.89 -3.5 5,423,735 - Pf6N2E2_4689 0.89 -2.7 5,423,767 + -1.6 5,423,767 + -0.4 5,423,767 + -3.5 5,423,768 - -2.4 5,423,768 - -2.1 5,423,768 - -1.6 5,423,804 - +0.9 5,423,804 - +1.6 5,423,804 - +1.3 5,423,815 + -0.9 5,423,816 - +1.9 5,423,866 - +1.6 5,423,878 + -2.8 5,423,878 + -3.5 5,423,878 + -2.7 5,423,892 + -3.8 5,423,893 - +1.8 5,423,893 - +0.9 5,423,893 - +1.0 5,423,893 - -0.8 5,424,066 - Pf6N2E2_4690 0.11 -4.3 5,424,066 - Pf6N2E2_4690 0.11 -4.1 5,424,066 - Pf6N2E2_4690 0.11 -3.5 5,424,142 + Pf6N2E2_4690 0.18 -3.3 5,424,142 + Pf6N2E2_4690 0.18 -3.7 5,424,142 + Pf6N2E2_4690 0.18 -3.3 5,424,143 - Pf6N2E2_4690 0.19 -1.3 5,424,143 - Pf6N2E2_4690 0.19 -3.7 5,424,220 + Pf6N2E2_4690 0.27 -2.5 5,424,220 + Pf6N2E2_4690 0.27 -3.3 5,424,220 + Pf6N2E2_4690 0.27 -3.8 5,424,221 - Pf6N2E2_4690 0.27 -3.8 5,424,221 - Pf6N2E2_4690 0.27 -3.6 5,424,221 - Pf6N2E2_4690 0.27 -4.6 5,424,415 + Pf6N2E2_4690 0.47 -2.9 5,424,416 - Pf6N2E2_4690 0.47 -3.5 5,424,416 - Pf6N2E2_4690 0.47 -3.7 5,424,416 - Pf6N2E2_4690 0.47 -3.6 5,424,490 + Pf6N2E2_4690 0.54 -3.5 5,424,491 - Pf6N2E2_4690 0.55 -4.8 5,424,547 + Pf6N2E2_4690 0.60 -3.8 5,424,548 - Pf6N2E2_4690 0.60 -3.7 5,424,575 + Pf6N2E2_4690 0.63 -4.0 5,424,575 + Pf6N2E2_4690 0.63 -2.7
Or see this region's nucleotide sequence