Experiment: Carnitine Hydrochloride (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2039 and Pf6N2E2_2040 are separated by 302 nucleotides Pf6N2E2_2040 and Pf6N2E2_2041 are separated by 7 nucleotides Pf6N2E2_2041 and Pf6N2E2_2042 are separated by 190 nucleotides
Pf6N2E2_2039 - Sigma-54 dependent transcriptional regulator, at 2,427,469 to 2,428,884
_2039
Pf6N2E2_2040 - D-beta-hydroxybutyrate permease, at 2,429,187 to 2,430,578
_2040
Pf6N2E2_2041 - D-beta-hydroxybutyrate dehydrogenase (EC 1.1.1.30), at 2,430,586 to 2,431,362
_2041
Pf6N2E2_2042 - Acetoacetyl-CoA synthetase (EC 6.2.1.16), at 2,431,553 to 2,433,508
_2042
Position (kb)
2429
2430
2431 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2428.228 kb on - strand, within Pf6N2E2_2039 at 2428.306 kb on + strand, within Pf6N2E2_2039 at 2428.417 kb on + strand, within Pf6N2E2_2039 at 2428.553 kb on + strand, within Pf6N2E2_2039 at 2428.554 kb on - strand, within Pf6N2E2_2039 at 2428.554 kb on - strand, within Pf6N2E2_2039 at 2428.677 kb on - strand, within Pf6N2E2_2039 at 2428.843 kb on + strand at 2428.844 kb on - strand at 2428.844 kb on - strand at 2428.882 kb on + strand at 2428.904 kb on + strand at 2428.906 kb on + strand at 2428.983 kb on - strand at 2429.167 kb on - strand at 2429.371 kb on - strand, within Pf6N2E2_2040 at 2429.468 kb on + strand, within Pf6N2E2_2040 at 2429.517 kb on + strand, within Pf6N2E2_2040 at 2429.517 kb on + strand, within Pf6N2E2_2040 at 2429.517 kb on + strand, within Pf6N2E2_2040 at 2429.518 kb on - strand, within Pf6N2E2_2040 at 2429.518 kb on - strand, within Pf6N2E2_2040 at 2429.518 kb on - strand, within Pf6N2E2_2040 at 2429.588 kb on + strand, within Pf6N2E2_2040 at 2429.588 kb on + strand, within Pf6N2E2_2040 at 2429.589 kb on - strand, within Pf6N2E2_2040 at 2429.589 kb on - strand, within Pf6N2E2_2040 at 2429.591 kb on + strand, within Pf6N2E2_2040 at 2429.591 kb on + strand, within Pf6N2E2_2040 at 2429.591 kb on + strand, within Pf6N2E2_2040 at 2429.591 kb on + strand, within Pf6N2E2_2040 at 2429.592 kb on - strand, within Pf6N2E2_2040 at 2429.592 kb on - strand, within Pf6N2E2_2040 at 2430.076 kb on + strand, within Pf6N2E2_2040 at 2430.214 kb on + strand, within Pf6N2E2_2040 at 2430.214 kb on + strand, within Pf6N2E2_2040 at 2430.452 kb on + strand at 2430.452 kb on + strand at 2430.453 kb on - strand at 2430.453 kb on - strand at 2430.512 kb on + strand at 2430.512 kb on + strand at 2430.513 kb on - strand at 2430.513 kb on - strand at 2430.559 kb on - strand at 2431.045 kb on + strand, within Pf6N2E2_2041 at 2431.046 kb on - strand, within Pf6N2E2_2041 at 2431.046 kb on - strand, within Pf6N2E2_2041 at 2431.046 kb on - strand, within Pf6N2E2_2041 at 2431.046 kb on - strand, within Pf6N2E2_2041 at 2431.046 kb on - strand, within Pf6N2E2_2041 at 2431.292 kb on + strand at 2431.293 kb on - strand at 2431.293 kb on - strand at 2431.293 kb on - strand at 2431.335 kb on + strand at 2431.336 kb on - strand at 2431.361 kb on - strand at 2431.498 kb on + strand at 2431.499 kb on - strand at 2431.502 kb on + strand at 2431.503 kb on - strand at 2431.503 kb on - strand at 2431.503 kb on - strand at 2431.503 kb on - strand at 2431.524 kb on + strand at 2431.524 kb on + strand at 2431.524 kb on + strand at 2431.525 kb on - strand at 2431.525 kb on - strand at 2431.525 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carnitine Hydrochloride (C) remove 2,428,228 - Pf6N2E2_2039 0.54 -1.6 2,428,306 + Pf6N2E2_2039 0.59 +1.6 2,428,417 + Pf6N2E2_2039 0.67 -1.5 2,428,553 + Pf6N2E2_2039 0.77 -1.0 2,428,554 - Pf6N2E2_2039 0.77 -1.9 2,428,554 - Pf6N2E2_2039 0.77 -1.2 2,428,677 - Pf6N2E2_2039 0.85 -0.2 2,428,843 + -1.4 2,428,844 - -1.7 2,428,844 - -0.5 2,428,882 + -0.2 2,428,904 + -0.2 2,428,906 + -1.0 2,428,983 - +0.2 2,429,167 - -0.7 2,429,371 - Pf6N2E2_2040 0.13 -2.1 2,429,468 + Pf6N2E2_2040 0.20 -1.0 2,429,517 + Pf6N2E2_2040 0.24 -1.1 2,429,517 + Pf6N2E2_2040 0.24 -0.9 2,429,517 + Pf6N2E2_2040 0.24 -0.3 2,429,518 - Pf6N2E2_2040 0.24 -0.2 2,429,518 - Pf6N2E2_2040 0.24 -0.4 2,429,518 - Pf6N2E2_2040 0.24 -1.2 2,429,588 + Pf6N2E2_2040 0.29 -1.2 2,429,588 + Pf6N2E2_2040 0.29 -0.7 2,429,589 - Pf6N2E2_2040 0.29 -0.1 2,429,589 - Pf6N2E2_2040 0.29 -0.4 2,429,591 + Pf6N2E2_2040 0.29 -0.9 2,429,591 + Pf6N2E2_2040 0.29 -1.7 2,429,591 + Pf6N2E2_2040 0.29 -2.0 2,429,591 + Pf6N2E2_2040 0.29 -1.1 2,429,592 - Pf6N2E2_2040 0.29 -2.1 2,429,592 - Pf6N2E2_2040 0.29 -0.8 2,430,076 + Pf6N2E2_2040 0.64 -0.6 2,430,214 + Pf6N2E2_2040 0.74 -1.3 2,430,214 + Pf6N2E2_2040 0.74 -0.5 2,430,452 + +0.2 2,430,452 + +1.7 2,430,453 - -1.8 2,430,453 - +0.6 2,430,512 + -0.1 2,430,512 + +0.9 2,430,513 - -1.0 2,430,513 - -1.1 2,430,559 - -1.9 2,431,045 + Pf6N2E2_2041 0.59 -0.2 2,431,046 - Pf6N2E2_2041 0.59 -2.7 2,431,046 - Pf6N2E2_2041 0.59 -3.2 2,431,046 - Pf6N2E2_2041 0.59 -1.0 2,431,046 - Pf6N2E2_2041 0.59 -1.4 2,431,046 - Pf6N2E2_2041 0.59 -1.6 2,431,292 + -0.2 2,431,293 - -2.0 2,431,293 - -3.5 2,431,293 - +0.6 2,431,335 + -0.1 2,431,336 - -1.0 2,431,361 - -1.9 2,431,498 + -0.4 2,431,499 - -4.3 2,431,502 + +0.9 2,431,503 - -3.0 2,431,503 - -2.0 2,431,503 - +0.2 2,431,503 - -0.4 2,431,524 + -1.1 2,431,524 + -0.5 2,431,524 + -0.6 2,431,525 - -0.8 2,431,525 - -1.9 2,431,525 - -1.7
Or see this region's nucleotide sequence