Experiment: R2A with Fusidic 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS12695 and CA265_RS12700 are separated by 405 nucleotides CA265_RS12700 and CA265_RS12705 are separated by 306 nucleotides
CA265_RS12695: CA265_RS12695 - scramblase, at 3,165,292 to 3,165,894
_RS12695
CA265_RS12700: CA265_RS12700 - RNA-binding protein, at 3,166,300 to 3,167,145
_RS12700
CA265_RS12705: CA265_RS12705 - hypothetical protein, at 3,167,452 to 3,168,123
_RS12705
Position (kb)
3166
3167
3168 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3165.337 kb on + strand at 3165.390 kb on + strand, within CA265_RS12695 at 3165.393 kb on - strand, within CA265_RS12695 at 3165.397 kb on - strand, within CA265_RS12695 at 3165.478 kb on - strand, within CA265_RS12695 at 3165.511 kb on + strand, within CA265_RS12695 at 3165.588 kb on + strand, within CA265_RS12695 at 3165.589 kb on - strand, within CA265_RS12695 at 3165.624 kb on + strand, within CA265_RS12695 at 3165.658 kb on - strand, within CA265_RS12695 at 3165.658 kb on - strand, within CA265_RS12695 at 3165.714 kb on + strand, within CA265_RS12695 at 3165.714 kb on + strand, within CA265_RS12695 at 3165.714 kb on + strand, within CA265_RS12695 at 3165.714 kb on + strand, within CA265_RS12695 at 3165.714 kb on + strand, within CA265_RS12695 at 3165.736 kb on + strand, within CA265_RS12695 at 3165.769 kb on - strand, within CA265_RS12695 at 3165.771 kb on + strand, within CA265_RS12695 at 3165.771 kb on + strand, within CA265_RS12695 at 3165.807 kb on + strand, within CA265_RS12695 at 3165.849 kb on + strand at 3165.850 kb on - strand at 3165.916 kb on + strand at 3165.917 kb on - strand at 3165.953 kb on + strand at 3165.988 kb on + strand at 3165.988 kb on + strand at 3165.991 kb on + strand at 3166.032 kb on + strand at 3166.102 kb on - strand at 3166.142 kb on - strand at 3166.156 kb on + strand at 3166.156 kb on + strand at 3166.161 kb on + strand at 3166.162 kb on - strand at 3166.197 kb on - strand at 3166.203 kb on + strand at 3166.203 kb on + strand at 3166.242 kb on + strand at 3166.297 kb on + strand at 3166.297 kb on + strand at 3166.358 kb on + strand at 3166.378 kb on - strand at 3166.403 kb on - strand, within CA265_RS12700 at 3166.553 kb on + strand, within CA265_RS12700 at 3166.585 kb on + strand, within CA265_RS12700 at 3166.585 kb on + strand, within CA265_RS12700 at 3166.585 kb on + strand, within CA265_RS12700 at 3166.585 kb on + strand, within CA265_RS12700 at 3166.585 kb on + strand, within CA265_RS12700 at 3166.585 kb on + strand, within CA265_RS12700 at 3166.586 kb on - strand, within CA265_RS12700 at 3166.586 kb on - strand, within CA265_RS12700 at 3166.586 kb on - strand, within CA265_RS12700 at 3166.630 kb on - strand, within CA265_RS12700 at 3166.630 kb on - strand, within CA265_RS12700 at 3166.634 kb on - strand, within CA265_RS12700 at 3166.634 kb on - strand, within CA265_RS12700 at 3166.645 kb on - strand, within CA265_RS12700 at 3166.663 kb on - strand, within CA265_RS12700 at 3166.687 kb on - strand, within CA265_RS12700 at 3166.742 kb on + strand, within CA265_RS12700 at 3166.743 kb on - strand, within CA265_RS12700 at 3166.755 kb on + strand, within CA265_RS12700 at 3166.797 kb on + strand, within CA265_RS12700 at 3166.797 kb on + strand, within CA265_RS12700 at 3166.797 kb on + strand, within CA265_RS12700 at 3166.798 kb on - strand, within CA265_RS12700 at 3166.801 kb on + strand, within CA265_RS12700 at 3166.801 kb on + strand, within CA265_RS12700 at 3166.801 kb on + strand, within CA265_RS12700 at 3166.816 kb on + strand, within CA265_RS12700 at 3166.833 kb on + strand, within CA265_RS12700 at 3166.839 kb on + strand, within CA265_RS12700 at 3166.959 kb on - strand, within CA265_RS12700 at 3166.985 kb on + strand, within CA265_RS12700 at 3167.024 kb on - strand, within CA265_RS12700 at 3167.077 kb on + strand at 3167.077 kb on + strand at 3167.078 kb on - strand at 3167.078 kb on - strand at 3167.079 kb on + strand at 3167.080 kb on - strand at 3167.124 kb on + strand at 3167.177 kb on + strand at 3167.180 kb on + strand at 3167.181 kb on - strand at 3167.187 kb on - strand at 3167.211 kb on - strand at 3167.256 kb on + strand at 3167.256 kb on + strand at 3167.258 kb on + strand at 3167.258 kb on + strand at 3167.355 kb on + strand at 3167.363 kb on + strand at 3167.363 kb on + strand at 3167.380 kb on - strand at 3167.463 kb on + strand at 3167.530 kb on + strand, within CA265_RS12705 at 3167.531 kb on - strand, within CA265_RS12705 at 3167.536 kb on - strand, within CA265_RS12705 at 3167.598 kb on + strand, within CA265_RS12705 at 3167.619 kb on + strand, within CA265_RS12705 at 3167.626 kb on + strand, within CA265_RS12705 at 3167.676 kb on + strand, within CA265_RS12705 at 3167.755 kb on + strand, within CA265_RS12705 at 3167.797 kb on + strand, within CA265_RS12705 at 3167.798 kb on - strand, within CA265_RS12705 at 3167.836 kb on + strand, within CA265_RS12705 at 3167.869 kb on - strand, within CA265_RS12705 at 3167.895 kb on + strand, within CA265_RS12705 at 3168.027 kb on + strand, within CA265_RS12705 at 3168.108 kb on + strand at 3168.125 kb on + strand at 3168.127 kb on + strand at 3168.139 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusidic 0.003 mg/ml remove 3,165,337 + -0.5 3,165,390 + CA265_RS12695 0.16 +0.1 3,165,393 - CA265_RS12695 0.17 +0.2 3,165,397 - CA265_RS12695 0.17 -0.8 3,165,478 - CA265_RS12695 0.31 +0.2 3,165,511 + CA265_RS12695 0.36 +0.4 3,165,588 + CA265_RS12695 0.49 +0.3 3,165,589 - CA265_RS12695 0.49 +1.1 3,165,624 + CA265_RS12695 0.55 -2.7 3,165,658 - CA265_RS12695 0.61 -2.0 3,165,658 - CA265_RS12695 0.61 -0.3 3,165,714 + CA265_RS12695 0.70 +0.0 3,165,714 + CA265_RS12695 0.70 +0.1 3,165,714 + CA265_RS12695 0.70 -1.2 3,165,714 + CA265_RS12695 0.70 +0.2 3,165,714 + CA265_RS12695 0.70 +0.6 3,165,736 + CA265_RS12695 0.74 -0.8 3,165,769 - CA265_RS12695 0.79 -0.1 3,165,771 + CA265_RS12695 0.79 -0.3 3,165,771 + CA265_RS12695 0.79 +0.5 3,165,807 + CA265_RS12695 0.85 +0.7 3,165,849 + -1.3 3,165,850 - +0.2 3,165,916 + -0.8 3,165,917 - -0.7 3,165,953 + -0.1 3,165,988 + -0.2 3,165,988 + -0.6 3,165,991 + +0.6 3,166,032 + +1.7 3,166,102 - +0.6 3,166,142 - +1.6 3,166,156 + -3.5 3,166,156 + -0.3 3,166,161 + -0.9 3,166,162 - -0.5 3,166,197 - -0.1 3,166,203 + +0.1 3,166,203 + +0.9 3,166,242 + -0.5 3,166,297 + -0.3 3,166,297 + -0.2 3,166,358 + -0.8 3,166,378 - +1.0 3,166,403 - CA265_RS12700 0.12 -0.4 3,166,553 + CA265_RS12700 0.30 -0.9 3,166,585 + CA265_RS12700 0.34 -0.6 3,166,585 + CA265_RS12700 0.34 +0.2 3,166,585 + CA265_RS12700 0.34 -1.0 3,166,585 + CA265_RS12700 0.34 -0.1 3,166,585 + CA265_RS12700 0.34 -0.2 3,166,585 + CA265_RS12700 0.34 -2.5 3,166,586 - CA265_RS12700 0.34 +0.5 3,166,586 - CA265_RS12700 0.34 -1.0 3,166,586 - CA265_RS12700 0.34 +0.2 3,166,630 - CA265_RS12700 0.39 -0.3 3,166,630 - CA265_RS12700 0.39 -0.1 3,166,634 - CA265_RS12700 0.39 -0.1 3,166,634 - CA265_RS12700 0.39 -0.1 3,166,645 - CA265_RS12700 0.41 -0.3 3,166,663 - CA265_RS12700 0.43 -0.5 3,166,687 - CA265_RS12700 0.46 -0.5 3,166,742 + CA265_RS12700 0.52 -1.3 3,166,743 - CA265_RS12700 0.52 -2.1 3,166,755 + CA265_RS12700 0.54 -0.2 3,166,797 + CA265_RS12700 0.59 -0.7 3,166,797 + CA265_RS12700 0.59 -1.1 3,166,797 + CA265_RS12700 0.59 +1.4 3,166,798 - CA265_RS12700 0.59 +0.6 3,166,801 + CA265_RS12700 0.59 -0.3 3,166,801 + CA265_RS12700 0.59 +0.2 3,166,801 + CA265_RS12700 0.59 +0.0 3,166,816 + CA265_RS12700 0.61 +0.0 3,166,833 + CA265_RS12700 0.63 -0.1 3,166,839 + CA265_RS12700 0.64 +0.8 3,166,959 - CA265_RS12700 0.78 +0.1 3,166,985 + CA265_RS12700 0.81 -1.0 3,167,024 - CA265_RS12700 0.86 +0.5 3,167,077 + +0.5 3,167,077 + -0.5 3,167,078 - -0.1 3,167,078 - -0.2 3,167,079 + -0.0 3,167,080 - +0.2 3,167,124 + +0.3 3,167,177 + +0.4 3,167,180 + -0.1 3,167,181 - -1.6 3,167,187 - -0.1 3,167,211 - -0.3 3,167,256 + +0.2 3,167,256 + -0.5 3,167,258 + -1.2 3,167,258 + +0.1 3,167,355 + -0.8 3,167,363 + -0.4 3,167,363 + -0.4 3,167,380 - +0.8 3,167,463 + +0.1 3,167,530 + CA265_RS12705 0.12 -0.1 3,167,531 - CA265_RS12705 0.12 -3.5 3,167,536 - CA265_RS12705 0.12 +0.9 3,167,598 + CA265_RS12705 0.22 +1.4 3,167,619 + CA265_RS12705 0.25 +0.4 3,167,626 + CA265_RS12705 0.26 -0.3 3,167,676 + CA265_RS12705 0.33 -0.5 3,167,755 + CA265_RS12705 0.45 +1.2 3,167,797 + CA265_RS12705 0.51 +0.4 3,167,798 - CA265_RS12705 0.51 +0.3 3,167,836 + CA265_RS12705 0.57 -0.5 3,167,869 - CA265_RS12705 0.62 +1.0 3,167,895 + CA265_RS12705 0.66 +0.9 3,168,027 + CA265_RS12705 0.86 -0.0 3,168,108 + -0.1 3,168,125 + +1.1 3,168,127 + +0.4 3,168,139 - -0.1
Or see this region's nucleotide sequence