Experiment: CDM1 without Cyanocobalamin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS17925 and BBR_RS17930 are separated by 66 nucleotides BBR_RS17930 and BBR_RS17935 are separated by 135 nucleotides BBR_RS17935 and BBR_RS17940 are separated by 136 nucleotides BBR_RS17940 and BBR_RS17945 are separated by 187 nucleotides
BBR_RS17925: BBR_RS17925 - exodeoxyribonuclease VII small subunit, at 1,809,032 to 1,809,316
_RS17925
BBR_RS17930: BBR_RS17930 - flavodoxin family protein, at 1,809,383 to 1,809,907
_RS17930
BBR_RS17935: BBR_RS17935 - long-chain fatty acid--CoA ligase, at 1,810,043 to 1,811,899
_RS17935
BBR_RS17940: BBR_RS17940 - large conductance mechanosensitive channel protein MscL, at 1,812,036 to 1,812,542
_RS17940
BBR_RS17945: BBR_RS17945 - nucleoside deoxyribosyltransferase, at 1,812,730 to 1,813,158
_RS17945
Position (kb)
1810
1811
1812 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1809.345 kb on + strand at 1809.349 kb on + strand at 1809.355 kb on - strand at 1809.384 kb on + strand at 1809.416 kb on - strand at 1809.426 kb on + strand at 1809.464 kb on + strand, within BBR_RS17930 at 1809.499 kb on - strand, within BBR_RS17930 at 1809.511 kb on - strand, within BBR_RS17930 at 1809.550 kb on + strand, within BBR_RS17930 at 1809.550 kb on - strand, within BBR_RS17930 at 1809.589 kb on + strand, within BBR_RS17930 at 1809.621 kb on - strand, within BBR_RS17930 at 1809.636 kb on - strand, within BBR_RS17930 at 1809.650 kb on - strand, within BBR_RS17930 at 1809.652 kb on + strand, within BBR_RS17930 at 1809.652 kb on + strand, within BBR_RS17930 at 1809.652 kb on + strand, within BBR_RS17930 at 1809.660 kb on - strand, within BBR_RS17930 at 1809.674 kb on - strand, within BBR_RS17930 at 1809.769 kb on + strand, within BBR_RS17930 at 1809.769 kb on + strand, within BBR_RS17930 at 1809.786 kb on - strand, within BBR_RS17930 at 1809.804 kb on + strand, within BBR_RS17930 at 1809.808 kb on + strand, within BBR_RS17930 at 1809.830 kb on + strand, within BBR_RS17930 at 1809.835 kb on + strand, within BBR_RS17930 at 1809.868 kb on - strand at 1809.869 kb on - strand at 1809.869 kb on - strand at 1809.869 kb on - strand at 1809.869 kb on - strand at 1809.885 kb on + strand at 1809.896 kb on + strand at 1809.904 kb on - strand at 1809.906 kb on + strand at 1809.928 kb on + strand at 1809.948 kb on - strand at 1809.948 kb on - strand at 1809.990 kb on - strand at 1809.996 kb on + strand at 1810.016 kb on - strand at 1810.035 kb on - strand at 1810.081 kb on + strand at 1810.081 kb on + strand at 1810.081 kb on + strand at 1810.089 kb on - strand at 1810.089 kb on - strand at 1810.091 kb on - strand at 1810.123 kb on + strand at 1810.148 kb on - strand at 1810.213 kb on - strand at 1810.296 kb on - strand, within BBR_RS17935 at 1810.326 kb on + strand, within BBR_RS17935 at 1810.334 kb on - strand, within BBR_RS17935 at 1810.377 kb on + strand, within BBR_RS17935 at 1810.396 kb on + strand, within BBR_RS17935 at 1810.396 kb on + strand, within BBR_RS17935 at 1810.403 kb on + strand, within BBR_RS17935 at 1810.411 kb on - strand, within BBR_RS17935 at 1810.463 kb on - strand, within BBR_RS17935 at 1810.463 kb on - strand, within BBR_RS17935 at 1810.476 kb on - strand, within BBR_RS17935 at 1810.497 kb on - strand, within BBR_RS17935 at 1810.539 kb on + strand, within BBR_RS17935 at 1810.558 kb on - strand, within BBR_RS17935 at 1810.564 kb on + strand, within BBR_RS17935 at 1810.577 kb on - strand, within BBR_RS17935 at 1810.577 kb on - strand, within BBR_RS17935 at 1810.584 kb on + strand, within BBR_RS17935 at 1810.592 kb on - strand, within BBR_RS17935 at 1810.600 kb on - strand, within BBR_RS17935 at 1810.600 kb on - strand, within BBR_RS17935 at 1810.610 kb on - strand, within BBR_RS17935 at 1810.610 kb on - strand, within BBR_RS17935 at 1810.711 kb on - strand, within BBR_RS17935 at 1810.716 kb on - strand, within BBR_RS17935 at 1810.720 kb on + strand, within BBR_RS17935 at 1810.742 kb on - strand, within BBR_RS17935 at 1810.761 kb on + strand, within BBR_RS17935 at 1810.797 kb on + strand, within BBR_RS17935 at 1810.805 kb on - strand, within BBR_RS17935 at 1810.825 kb on + strand, within BBR_RS17935 at 1810.825 kb on + strand, within BBR_RS17935 at 1810.920 kb on + strand, within BBR_RS17935 at 1810.920 kb on + strand, within BBR_RS17935 at 1810.920 kb on + strand, within BBR_RS17935 at 1810.920 kb on + strand, within BBR_RS17935 at 1810.920 kb on + strand, within BBR_RS17935 at 1810.928 kb on - strand, within BBR_RS17935 at 1810.928 kb on - strand, within BBR_RS17935 at 1810.928 kb on - strand, within BBR_RS17935 at 1810.928 kb on - strand, within BBR_RS17935 at 1810.928 kb on - strand, within BBR_RS17935 at 1810.955 kb on + strand, within BBR_RS17935 at 1811.012 kb on - strand, within BBR_RS17935 at 1811.012 kb on - strand, within BBR_RS17935 at 1811.042 kb on - strand, within BBR_RS17935 at 1811.066 kb on + strand, within BBR_RS17935 at 1811.079 kb on + strand, within BBR_RS17935 at 1811.116 kb on + strand, within BBR_RS17935 at 1811.116 kb on + strand, within BBR_RS17935 at 1811.116 kb on + strand, within BBR_RS17935 at 1811.154 kb on + strand, within BBR_RS17935 at 1811.163 kb on + strand, within BBR_RS17935 at 1811.163 kb on + strand, within BBR_RS17935 at 1811.164 kb on - strand, within BBR_RS17935 at 1811.171 kb on - strand, within BBR_RS17935 at 1811.171 kb on - strand, within BBR_RS17935 at 1811.179 kb on - strand, within BBR_RS17935 at 1811.208 kb on + strand, within BBR_RS17935 at 1811.208 kb on + strand, within BBR_RS17935 at 1811.213 kb on + strand, within BBR_RS17935 at 1811.216 kb on + strand, within BBR_RS17935 at 1811.221 kb on - strand, within BBR_RS17935 at 1811.224 kb on - strand, within BBR_RS17935 at 1811.423 kb on + strand, within BBR_RS17935 at 1811.428 kb on + strand, within BBR_RS17935 at 1811.428 kb on + strand, within BBR_RS17935 at 1811.431 kb on - strand, within BBR_RS17935 at 1811.431 kb on - strand, within BBR_RS17935 at 1811.431 kb on - strand, within BBR_RS17935 at 1811.431 kb on - strand, within BBR_RS17935 at 1811.451 kb on + strand, within BBR_RS17935 at 1811.472 kb on + strand, within BBR_RS17935 at 1811.500 kb on + strand, within BBR_RS17935 at 1811.500 kb on + strand, within BBR_RS17935 at 1811.533 kb on - strand, within BBR_RS17935 at 1811.586 kb on + strand, within BBR_RS17935 at 1811.591 kb on + strand, within BBR_RS17935 at 1811.591 kb on + strand, within BBR_RS17935 at 1811.591 kb on + strand, within BBR_RS17935 at 1811.599 kb on - strand, within BBR_RS17935 at 1811.606 kb on + strand, within BBR_RS17935 at 1811.609 kb on - strand, within BBR_RS17935 at 1811.637 kb on + strand, within BBR_RS17935 at 1811.668 kb on + strand, within BBR_RS17935 at 1811.670 kb on + strand, within BBR_RS17935 at 1811.671 kb on - strand, within BBR_RS17935 at 1811.671 kb on - strand, within BBR_RS17935 at 1811.678 kb on - strand, within BBR_RS17935 at 1811.710 kb on - strand, within BBR_RS17935 at 1811.756 kb on + strand at 1811.803 kb on + strand at 1811.811 kb on - strand at 1811.813 kb on - strand at 1811.835 kb on + strand at 1811.835 kb on - strand at 1811.848 kb on + strand at 1811.873 kb on - strand at 1811.921 kb on + strand at 1811.964 kb on - strand at 1811.964 kb on - strand at 1811.965 kb on + strand at 1811.973 kb on - strand at 1812.028 kb on + strand at 1812.028 kb on + strand at 1812.035 kb on - strand at 1812.035 kb on - strand at 1812.036 kb on - strand at 1812.036 kb on - strand at 1812.036 kb on - strand at 1812.096 kb on + strand, within BBR_RS17940 at 1812.110 kb on + strand, within BBR_RS17940 at 1812.137 kb on - strand, within BBR_RS17940 at 1812.144 kb on - strand, within BBR_RS17940 at 1812.148 kb on - strand, within BBR_RS17940 at 1812.149 kb on - strand, within BBR_RS17940 at 1812.177 kb on + strand, within BBR_RS17940 at 1812.200 kb on - strand, within BBR_RS17940 at 1812.239 kb on + strand, within BBR_RS17940 at 1812.242 kb on - strand, within BBR_RS17940 at 1812.247 kb on - strand, within BBR_RS17940 at 1812.247 kb on - strand, within BBR_RS17940 at 1812.282 kb on - strand, within BBR_RS17940 at 1812.378 kb on + strand, within BBR_RS17940 at 1812.378 kb on + strand, within BBR_RS17940 at 1812.378 kb on + strand, within BBR_RS17940 at 1812.386 kb on - strand, within BBR_RS17940 at 1812.386 kb on - strand, within BBR_RS17940 at 1812.386 kb on - strand, within BBR_RS17940 at 1812.386 kb on - strand, within BBR_RS17940 at 1812.386 kb on - strand, within BBR_RS17940 at 1812.449 kb on + strand, within BBR_RS17940 at 1812.489 kb on + strand, within BBR_RS17940 at 1812.536 kb on - strand at 1812.548 kb on + strand at 1812.548 kb on + strand at 1812.556 kb on - strand at 1812.698 kb on + strand at 1812.706 kb on - strand at 1812.808 kb on + strand, within BBR_RS17945 at 1812.852 kb on + strand, within BBR_RS17945 at 1812.852 kb on + strand, within BBR_RS17945 at 1812.897 kb on + strand, within BBR_RS17945
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Cyanocobalamin remove 1,809,345 + +0.7 1,809,349 + -0.1 1,809,355 - -0.3 1,809,384 + -0.2 1,809,416 - -2.8 1,809,426 + -1.5 1,809,464 + BBR_RS17930 0.15 -0.8 1,809,499 - BBR_RS17930 0.22 -2.5 1,809,511 - BBR_RS17930 0.24 -2.9 1,809,550 + BBR_RS17930 0.32 -0.4 1,809,550 - BBR_RS17930 0.32 -1.5 1,809,589 + BBR_RS17930 0.39 -0.4 1,809,621 - BBR_RS17930 0.45 -1.4 1,809,636 - BBR_RS17930 0.48 -1.7 1,809,650 - BBR_RS17930 0.51 -0.8 1,809,652 + BBR_RS17930 0.51 -0.4 1,809,652 + BBR_RS17930 0.51 -0.4 1,809,652 + BBR_RS17930 0.51 -0.7 1,809,660 - BBR_RS17930 0.53 -1.2 1,809,674 - BBR_RS17930 0.55 -0.1 1,809,769 + BBR_RS17930 0.74 -1.5 1,809,769 + BBR_RS17930 0.74 +0.9 1,809,786 - BBR_RS17930 0.77 -2.3 1,809,804 + BBR_RS17930 0.80 -0.2 1,809,808 + BBR_RS17930 0.81 -3.1 1,809,830 + BBR_RS17930 0.85 -1.2 1,809,835 + BBR_RS17930 0.86 -1.3 1,809,868 - -1.2 1,809,869 - -1.8 1,809,869 - -1.4 1,809,869 - +0.7 1,809,869 - -1.6 1,809,885 + -1.5 1,809,896 + -1.2 1,809,904 - -3.1 1,809,906 + -0.0 1,809,928 + +0.7 1,809,948 - -0.0 1,809,948 - +0.9 1,809,990 - +1.6 1,809,996 + -0.1 1,810,016 - -0.7 1,810,035 - +0.1 1,810,081 + -2.6 1,810,081 + -0.1 1,810,081 + -0.8 1,810,089 - -0.7 1,810,089 - -1.4 1,810,091 - -0.7 1,810,123 + -0.7 1,810,148 - -0.5 1,810,213 - -0.3 1,810,296 - BBR_RS17935 0.14 -0.4 1,810,326 + BBR_RS17935 0.15 -2.5 1,810,334 - BBR_RS17935 0.16 -1.7 1,810,377 + BBR_RS17935 0.18 -0.2 1,810,396 + BBR_RS17935 0.19 -1.0 1,810,396 + BBR_RS17935 0.19 -1.7 1,810,403 + BBR_RS17935 0.19 -0.1 1,810,411 - BBR_RS17935 0.20 -0.9 1,810,463 - BBR_RS17935 0.23 -1.1 1,810,463 - BBR_RS17935 0.23 -0.9 1,810,476 - BBR_RS17935 0.23 -1.7 1,810,497 - BBR_RS17935 0.24 -1.4 1,810,539 + BBR_RS17935 0.27 -1.6 1,810,558 - BBR_RS17935 0.28 -3.5 1,810,564 + BBR_RS17935 0.28 -0.6 1,810,577 - BBR_RS17935 0.29 -0.7 1,810,577 - BBR_RS17935 0.29 +0.1 1,810,584 + BBR_RS17935 0.29 -0.3 1,810,592 - BBR_RS17935 0.30 -0.1 1,810,600 - BBR_RS17935 0.30 -1.6 1,810,600 - BBR_RS17935 0.30 -2.4 1,810,610 - BBR_RS17935 0.31 -1.2 1,810,610 - BBR_RS17935 0.31 +0.8 1,810,711 - BBR_RS17935 0.36 -3.1 1,810,716 - BBR_RS17935 0.36 -0.9 1,810,720 + BBR_RS17935 0.36 -2.0 1,810,742 - BBR_RS17935 0.38 -0.8 1,810,761 + BBR_RS17935 0.39 +0.1 1,810,797 + BBR_RS17935 0.41 -0.5 1,810,805 - BBR_RS17935 0.41 +0.3 1,810,825 + BBR_RS17935 0.42 -1.0 1,810,825 + BBR_RS17935 0.42 -2.4 1,810,920 + BBR_RS17935 0.47 -1.2 1,810,920 + BBR_RS17935 0.47 -1.9 1,810,920 + BBR_RS17935 0.47 -1.3 1,810,920 + BBR_RS17935 0.47 -1.8 1,810,920 + BBR_RS17935 0.47 -0.5 1,810,928 - BBR_RS17935 0.48 +0.6 1,810,928 - BBR_RS17935 0.48 -0.8 1,810,928 - BBR_RS17935 0.48 -0.3 1,810,928 - BBR_RS17935 0.48 -0.5 1,810,928 - BBR_RS17935 0.48 +0.4 1,810,955 + BBR_RS17935 0.49 -1.7 1,811,012 - BBR_RS17935 0.52 +0.2 1,811,012 - BBR_RS17935 0.52 -3.3 1,811,042 - BBR_RS17935 0.54 -0.6 1,811,066 + BBR_RS17935 0.55 -2.3 1,811,079 + BBR_RS17935 0.56 -0.2 1,811,116 + BBR_RS17935 0.58 -1.7 1,811,116 + BBR_RS17935 0.58 -1.4 1,811,116 + BBR_RS17935 0.58 -1.1 1,811,154 + BBR_RS17935 0.60 -0.4 1,811,163 + BBR_RS17935 0.60 -0.8 1,811,163 + BBR_RS17935 0.60 -0.8 1,811,164 - BBR_RS17935 0.60 -2.2 1,811,171 - BBR_RS17935 0.61 +0.1 1,811,171 - BBR_RS17935 0.61 -0.3 1,811,179 - BBR_RS17935 0.61 -0.2 1,811,208 + BBR_RS17935 0.63 -1.7 1,811,208 + BBR_RS17935 0.63 -0.9 1,811,213 + BBR_RS17935 0.63 -0.7 1,811,216 + BBR_RS17935 0.63 -0.6 1,811,221 - BBR_RS17935 0.63 -0.3 1,811,224 - BBR_RS17935 0.64 -0.0 1,811,423 + BBR_RS17935 0.74 -1.1 1,811,428 + BBR_RS17935 0.75 +0.3 1,811,428 + BBR_RS17935 0.75 -2.4 1,811,431 - BBR_RS17935 0.75 -0.6 1,811,431 - BBR_RS17935 0.75 -1.0 1,811,431 - BBR_RS17935 0.75 +1.6 1,811,431 - BBR_RS17935 0.75 +0.4 1,811,451 + BBR_RS17935 0.76 +1.2 1,811,472 + BBR_RS17935 0.77 -0.1 1,811,500 + BBR_RS17935 0.78 -1.3 1,811,500 + BBR_RS17935 0.78 -0.0 1,811,533 - BBR_RS17935 0.80 -0.7 1,811,586 + BBR_RS17935 0.83 -1.1 1,811,591 + BBR_RS17935 0.83 -0.7 1,811,591 + BBR_RS17935 0.83 -2.1 1,811,591 + BBR_RS17935 0.83 -3.7 1,811,599 - BBR_RS17935 0.84 -0.9 1,811,606 + BBR_RS17935 0.84 -0.6 1,811,609 - BBR_RS17935 0.84 -3.0 1,811,637 + BBR_RS17935 0.86 +0.9 1,811,668 + BBR_RS17935 0.88 -0.7 1,811,670 + BBR_RS17935 0.88 +0.0 1,811,671 - BBR_RS17935 0.88 -0.3 1,811,671 - BBR_RS17935 0.88 -3.3 1,811,678 - BBR_RS17935 0.88 -2.2 1,811,710 - BBR_RS17935 0.90 -2.6 1,811,756 + +0.3 1,811,803 + -0.3 1,811,811 - -0.8 1,811,813 - -0.7 1,811,835 + -1.2 1,811,835 - -0.3 1,811,848 + -1.2 1,811,873 - -2.5 1,811,921 + -1.1 1,811,964 - -1.5 1,811,964 - -2.0 1,811,965 + +0.1 1,811,973 - -1.2 1,812,028 + +0.2 1,812,028 + +0.0 1,812,035 - +0.1 1,812,035 - +0.1 1,812,036 - +0.3 1,812,036 - -1.5 1,812,036 - -0.7 1,812,096 + BBR_RS17940 0.12 -1.3 1,812,110 + BBR_RS17940 0.15 -1.8 1,812,137 - BBR_RS17940 0.20 -1.1 1,812,144 - BBR_RS17940 0.21 -0.8 1,812,148 - BBR_RS17940 0.22 +1.0 1,812,149 - BBR_RS17940 0.22 +0.0 1,812,177 + BBR_RS17940 0.28 -0.3 1,812,200 - BBR_RS17940 0.32 -0.8 1,812,239 + BBR_RS17940 0.40 +0.6 1,812,242 - BBR_RS17940 0.41 -0.0 1,812,247 - BBR_RS17940 0.42 +3.0 1,812,247 - BBR_RS17940 0.42 +0.2 1,812,282 - BBR_RS17940 0.49 +0.0 1,812,378 + BBR_RS17940 0.67 -0.7 1,812,378 + BBR_RS17940 0.67 -1.2 1,812,378 + BBR_RS17940 0.67 +0.7 1,812,386 - BBR_RS17940 0.69 -0.8 1,812,386 - BBR_RS17940 0.69 +0.8 1,812,386 - BBR_RS17940 0.69 -1.1 1,812,386 - BBR_RS17940 0.69 +0.0 1,812,386 - BBR_RS17940 0.69 -3.3 1,812,449 + BBR_RS17940 0.81 +0.1 1,812,489 + BBR_RS17940 0.89 -2.1 1,812,536 - +0.7 1,812,548 + -1.5 1,812,548 + -1.3 1,812,556 - +0.4 1,812,698 + -1.0 1,812,706 - +0.2 1,812,808 + BBR_RS17945 0.18 -0.5 1,812,852 + BBR_RS17945 0.28 -0.9 1,812,852 + BBR_RS17945 0.28 +0.3 1,812,897 + BBR_RS17945 0.39 +0.2
Or see this region's nucleotide sequence