Experiment: CDM1 without Cyanocobalamin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15665 and BBR_RS15670 are separated by 50 nucleotides BBR_RS15670 and BBR_RS15675 are separated by 84 nucleotides BBR_RS15675 and BBR_RS15680 overlap by 1 nucleotides BBR_RS15680 and BBR_RS15685 are separated by 19 nucleotides
BBR_RS15665: BBR_RS15665 - preprotein translocase subunit YajC, at 1,284,253 to 1,284,699
_RS15665
BBR_RS15670: BBR_RS15670 - adenine phosphoribosyltransferase, at 1,284,750 to 1,285,331
_RS15670
BBR_RS15675: BBR_RS15675 - ADP-forming succinate--CoA ligase subunit beta, at 1,285,416 to 1,286,618
_RS15675
BBR_RS15680: BBR_RS15680 - succinate--CoA ligase subunit alpha, at 1,286,618 to 1,287,529
_RS15680
BBR_RS15685: BBR_RS15685 - hypothetical protein, at 1,287,549 to 1,288,934
_RS15685
Position (kb)
1285
1286
1287 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1284.561 kb on + strand, within BBR_RS15665 at 1284.645 kb on + strand, within BBR_RS15665 at 1284.777 kb on + strand at 1284.782 kb on + strand at 1284.795 kb on - strand at 1284.942 kb on + strand, within BBR_RS15670 at 1284.999 kb on + strand, within BBR_RS15670 at 1285.012 kb on + strand, within BBR_RS15670 at 1285.012 kb on - strand, within BBR_RS15670 at 1285.139 kb on - strand, within BBR_RS15670 at 1285.151 kb on + strand, within BBR_RS15670 at 1285.233 kb on + strand, within BBR_RS15670 at 1285.274 kb on - strand at 1285.405 kb on + strand at 1285.419 kb on - strand at 1285.422 kb on - strand at 1285.422 kb on - strand at 1285.463 kb on + strand at 1285.473 kb on - strand at 1285.473 kb on - strand at 1285.473 kb on - strand at 1285.580 kb on + strand, within BBR_RS15675 at 1285.597 kb on - strand, within BBR_RS15675 at 1285.676 kb on - strand, within BBR_RS15675 at 1285.676 kb on - strand, within BBR_RS15675 at 1285.693 kb on - strand, within BBR_RS15675 at 1285.711 kb on + strand, within BBR_RS15675 at 1285.805 kb on + strand, within BBR_RS15675 at 1285.835 kb on - strand, within BBR_RS15675 at 1285.906 kb on - strand, within BBR_RS15675 at 1285.951 kb on + strand, within BBR_RS15675 at 1286.151 kb on - strand, within BBR_RS15675 at 1286.158 kb on - strand, within BBR_RS15675 at 1286.188 kb on + strand, within BBR_RS15675 at 1286.203 kb on + strand, within BBR_RS15675 at 1286.203 kb on + strand, within BBR_RS15675 at 1286.211 kb on - strand, within BBR_RS15675 at 1286.211 kb on - strand, within BBR_RS15675 at 1286.211 kb on - strand, within BBR_RS15675 at 1286.213 kb on + strand, within BBR_RS15675 at 1286.214 kb on + strand, within BBR_RS15675 at 1286.214 kb on + strand, within BBR_RS15675 at 1286.375 kb on - strand, within BBR_RS15675 at 1286.406 kb on + strand, within BBR_RS15675 at 1286.458 kb on - strand, within BBR_RS15675 at 1286.490 kb on + strand, within BBR_RS15675 at 1286.498 kb on - strand at 1286.584 kb on + strand at 1286.597 kb on - strand at 1286.600 kb on + strand at 1286.600 kb on + strand at 1286.600 kb on + strand at 1286.632 kb on + strand at 1286.656 kb on + strand at 1286.656 kb on + strand at 1286.656 kb on + strand at 1286.656 kb on + strand at 1286.664 kb on - strand at 1286.664 kb on - strand at 1286.688 kb on - strand at 1286.688 kb on - strand at 1286.701 kb on - strand at 1286.735 kb on - strand, within BBR_RS15680 at 1286.750 kb on - strand, within BBR_RS15680 at 1286.858 kb on - strand, within BBR_RS15680 at 1286.973 kb on + strand, within BBR_RS15680 at 1286.983 kb on + strand, within BBR_RS15680 at 1286.983 kb on + strand, within BBR_RS15680 at 1286.983 kb on + strand, within BBR_RS15680 at 1286.983 kb on + strand, within BBR_RS15680 at 1286.983 kb on + strand, within BBR_RS15680 at 1286.990 kb on + strand, within BBR_RS15680 at 1287.003 kb on - strand, within BBR_RS15680 at 1287.023 kb on + strand, within BBR_RS15680 at 1287.095 kb on + strand, within BBR_RS15680 at 1287.124 kb on - strand, within BBR_RS15680 at 1287.177 kb on - strand, within BBR_RS15680 at 1287.177 kb on - strand, within BBR_RS15680 at 1287.198 kb on + strand, within BBR_RS15680 at 1287.198 kb on + strand, within BBR_RS15680 at 1287.198 kb on + strand, within BBR_RS15680 at 1287.198 kb on + strand, within BBR_RS15680 at 1287.206 kb on - strand, within BBR_RS15680 at 1287.206 kb on - strand, within BBR_RS15680 at 1287.307 kb on + strand, within BBR_RS15680 at 1287.461 kb on - strand at 1287.464 kb on + strand at 1287.471 kb on - strand at 1287.483 kb on - strand at 1287.498 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Cyanocobalamin remove 1,284,561 + BBR_RS15665 0.69 +0.6 1,284,645 + BBR_RS15665 0.88 +0.4 1,284,777 + -0.4 1,284,782 + -2.1 1,284,795 - -1.0 1,284,942 + BBR_RS15670 0.33 +0.5 1,284,999 + BBR_RS15670 0.43 -0.5 1,285,012 + BBR_RS15670 0.45 -0.3 1,285,012 - BBR_RS15670 0.45 -2.0 1,285,139 - BBR_RS15670 0.67 -1.3 1,285,151 + BBR_RS15670 0.69 +0.4 1,285,233 + BBR_RS15670 0.83 -0.7 1,285,274 - -1.9 1,285,405 + -2.0 1,285,419 - -2.6 1,285,422 - -2.9 1,285,422 - -2.7 1,285,463 + -5.1 1,285,473 - -2.9 1,285,473 - -2.5 1,285,473 - -3.1 1,285,580 + BBR_RS15675 0.14 -2.5 1,285,597 - BBR_RS15675 0.15 -1.5 1,285,676 - BBR_RS15675 0.22 -1.1 1,285,676 - BBR_RS15675 0.22 -2.0 1,285,693 - BBR_RS15675 0.23 -3.2 1,285,711 + BBR_RS15675 0.25 -3.1 1,285,805 + BBR_RS15675 0.32 -4.9 1,285,835 - BBR_RS15675 0.35 +0.1 1,285,906 - BBR_RS15675 0.41 -0.6 1,285,951 + BBR_RS15675 0.44 -3.3 1,286,151 - BBR_RS15675 0.61 -1.6 1,286,158 - BBR_RS15675 0.62 -1.9 1,286,188 + BBR_RS15675 0.64 -1.7 1,286,203 + BBR_RS15675 0.65 -1.8 1,286,203 + BBR_RS15675 0.65 -3.2 1,286,211 - BBR_RS15675 0.66 -1.9 1,286,211 - BBR_RS15675 0.66 -2.4 1,286,211 - BBR_RS15675 0.66 -2.4 1,286,213 + BBR_RS15675 0.66 -2.6 1,286,214 + BBR_RS15675 0.66 -1.6 1,286,214 + BBR_RS15675 0.66 -2.1 1,286,375 - BBR_RS15675 0.80 -0.6 1,286,406 + BBR_RS15675 0.82 -3.3 1,286,458 - BBR_RS15675 0.87 -2.0 1,286,490 + BBR_RS15675 0.89 -0.3 1,286,498 - -3.5 1,286,584 + +2.3 1,286,597 - +1.3 1,286,600 + +2.5 1,286,600 + +0.3 1,286,600 + +0.2 1,286,632 + -0.7 1,286,656 + -2.1 1,286,656 + -3.4 1,286,656 + -2.4 1,286,656 + -2.5 1,286,664 - +1.3 1,286,664 - +1.9 1,286,688 - -2.1 1,286,688 - -2.9 1,286,701 - -2.3 1,286,735 - BBR_RS15680 0.13 -0.9 1,286,750 - BBR_RS15680 0.14 -1.7 1,286,858 - BBR_RS15680 0.26 -3.0 1,286,973 + BBR_RS15680 0.39 -2.0 1,286,983 + BBR_RS15680 0.40 -0.7 1,286,983 + BBR_RS15680 0.40 -2.9 1,286,983 + BBR_RS15680 0.40 -1.1 1,286,983 + BBR_RS15680 0.40 -2.0 1,286,983 + BBR_RS15680 0.40 -3.3 1,286,990 + BBR_RS15680 0.41 -3.2 1,287,003 - BBR_RS15680 0.42 -1.6 1,287,023 + BBR_RS15680 0.44 -3.4 1,287,095 + BBR_RS15680 0.52 -4.8 1,287,124 - BBR_RS15680 0.55 -2.1 1,287,177 - BBR_RS15680 0.61 -2.8 1,287,177 - BBR_RS15680 0.61 -2.2 1,287,198 + BBR_RS15680 0.64 -3.7 1,287,198 + BBR_RS15680 0.64 -4.0 1,287,198 + BBR_RS15680 0.64 -0.9 1,287,198 + BBR_RS15680 0.64 +0.9 1,287,206 - BBR_RS15680 0.64 -2.7 1,287,206 - BBR_RS15680 0.64 -3.2 1,287,307 + BBR_RS15680 0.76 -1.2 1,287,461 - -1.3 1,287,464 + -1.8 1,287,471 - -1.1 1,287,483 - -2.7 1,287,498 - -0.8
Or see this region's nucleotide sequence