Experiment: Cisplatin 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_68 and Ac3H11_69 are separated by 16 nucleotides Ac3H11_69 and Ac3H11_70 are separated by 93 nucleotides
Ac3H11_68 - High-affinity branched-chain amino acid transport system permease protein LivH (TC 3.A.1.4.1), at 70,564 to 71,550
_68
Ac3H11_69 - Branched-chain amino acid transport system permease protein LivM (TC 3.A.1.4.1), at 71,567 to 72,640
_69
Ac3H11_70 - D(-)-3-hydroxybutyrate oligomer hydrolase (EC 3.1.1.22), at 72,734 to 74,878
_70
Position (kb)
71
72
73 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 70.826 kb on - strand, within Ac3H11_68 at 71.443 kb on + strand, within Ac3H11_68 at 71.444 kb on - strand, within Ac3H11_68 at 71.488 kb on - strand at 71.750 kb on + strand, within Ac3H11_69 at 71.751 kb on - strand, within Ac3H11_69 at 72.116 kb on + strand, within Ac3H11_69 at 72.116 kb on + strand, within Ac3H11_69 at 72.117 kb on - strand, within Ac3H11_69 at 72.119 kb on + strand, within Ac3H11_69 at 72.119 kb on + strand, within Ac3H11_69 at 72.119 kb on + strand, within Ac3H11_69 at 72.254 kb on + strand, within Ac3H11_69 at 72.255 kb on - strand, within Ac3H11_69 at 72.329 kb on + strand, within Ac3H11_69 at 72.330 kb on - strand, within Ac3H11_69 at 72.414 kb on - strand, within Ac3H11_69 at 72.414 kb on - strand, within Ac3H11_69 at 72.459 kb on - strand, within Ac3H11_69 at 72.578 kb on + strand at 72.579 kb on - strand at 72.579 kb on - strand at 72.884 kb on + strand at 72.884 kb on + strand at 72.884 kb on + strand at 72.884 kb on + strand at 72.884 kb on + strand at 72.885 kb on - strand at 73.068 kb on - strand, within Ac3H11_70 at 73.260 kb on - strand, within Ac3H11_70 at 73.260 kb on - strand, within Ac3H11_70 at 73.314 kb on - strand, within Ac3H11_70 at 73.565 kb on + strand, within Ac3H11_70 at 73.565 kb on + strand, within Ac3H11_70 at 73.565 kb on + strand, within Ac3H11_70 at 73.565 kb on + strand, within Ac3H11_70 at 73.565 kb on + strand, within Ac3H11_70 at 73.565 kb on + strand, within Ac3H11_70 at 73.566 kb on - strand, within Ac3H11_70 at 73.566 kb on - strand, within Ac3H11_70
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.005 mg/ml remove 70,826 - Ac3H11_68 0.27 -0.7 71,443 + Ac3H11_68 0.89 -1.8 71,444 - Ac3H11_68 0.89 +1.5 71,488 - +1.7 71,750 + Ac3H11_69 0.17 +1.5 71,751 - Ac3H11_69 0.17 -0.1 72,116 + Ac3H11_69 0.51 +1.7 72,116 + Ac3H11_69 0.51 +1.1 72,117 - Ac3H11_69 0.51 -2.9 72,119 + Ac3H11_69 0.51 +1.1 72,119 + Ac3H11_69 0.51 -1.5 72,119 + Ac3H11_69 0.51 +0.8 72,254 + Ac3H11_69 0.64 -1.8 72,255 - Ac3H11_69 0.64 +3.8 72,329 + Ac3H11_69 0.71 -1.9 72,330 - Ac3H11_69 0.71 +2.5 72,414 - Ac3H11_69 0.79 +0.3 72,414 - Ac3H11_69 0.79 +2.1 72,459 - Ac3H11_69 0.83 +0.1 72,578 + +0.8 72,579 - -0.7 72,579 - +1.2 72,884 + -1.0 72,884 + -0.0 72,884 + +1.2 72,884 + -2.0 72,884 + -0.6 72,885 - +1.0 73,068 - Ac3H11_70 0.16 +2.0 73,260 - Ac3H11_70 0.25 -0.5 73,260 - Ac3H11_70 0.25 -3.2 73,314 - Ac3H11_70 0.27 -1.2 73,565 + Ac3H11_70 0.39 +0.1 73,565 + Ac3H11_70 0.39 +1.9 73,565 + Ac3H11_70 0.39 -1.8 73,565 + Ac3H11_70 0.39 -1.3 73,565 + Ac3H11_70 0.39 +2.1 73,565 + Ac3H11_70 0.39 +0.3 73,566 - Ac3H11_70 0.39 -0.5 73,566 - Ac3H11_70 0.39 -2.4
Or see this region's nucleotide sequence