Experiment: Cisplatin 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_4399 and Ac3H11_4400 are separated by 146 nucleotides Ac3H11_4400 and Ac3H11_4401 are separated by 50 nucleotides
Ac3H11_4399 - Tricarboxylate transport protein TctC, at 195,709 to 196,686
_4399
Ac3H11_4400 - putative LysR-family transcriptional regulator, at 196,833 to 197,729
_4400
Ac3H11_4401 - Orotidine 5'-phosphate decarboxylase (EC 4.1.1.23), at 197,780 to 198,607
_4401
Position (kb)
196
197
198 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 195.947 kb on + strand, within Ac3H11_4399 at 195.947 kb on + strand, within Ac3H11_4399 at 195.948 kb on - strand, within Ac3H11_4399 at 195.948 kb on - strand, within Ac3H11_4399 at 196.076 kb on + strand, within Ac3H11_4399 at 196.128 kb on - strand, within Ac3H11_4399 at 196.214 kb on + strand, within Ac3H11_4399 at 196.214 kb on + strand, within Ac3H11_4399 at 196.215 kb on - strand, within Ac3H11_4399 at 196.215 kb on - strand, within Ac3H11_4399 at 196.215 kb on - strand, within Ac3H11_4399 at 196.215 kb on - strand, within Ac3H11_4399 at 196.349 kb on + strand, within Ac3H11_4399 at 196.349 kb on + strand, within Ac3H11_4399 at 196.349 kb on + strand, within Ac3H11_4399 at 196.349 kb on + strand, within Ac3H11_4399 at 196.349 kb on + strand, within Ac3H11_4399 at 196.349 kb on + strand, within Ac3H11_4399 at 196.350 kb on - strand, within Ac3H11_4399 at 196.350 kb on - strand, within Ac3H11_4399 at 196.350 kb on - strand, within Ac3H11_4399 at 196.350 kb on - strand, within Ac3H11_4399 at 196.415 kb on + strand, within Ac3H11_4399 at 196.416 kb on - strand, within Ac3H11_4399 at 196.416 kb on - strand, within Ac3H11_4399 at 196.552 kb on - strand, within Ac3H11_4399 at 197.020 kb on + strand, within Ac3H11_4400 at 197.020 kb on + strand, within Ac3H11_4400 at 197.020 kb on + strand, within Ac3H11_4400 at 197.020 kb on + strand, within Ac3H11_4400 at 197.020 kb on + strand, within Ac3H11_4400 at 197.020 kb on + strand, within Ac3H11_4400 at 197.020 kb on + strand, within Ac3H11_4400 at 197.020 kb on + strand, within Ac3H11_4400 at 197.020 kb on + strand, within Ac3H11_4400 at 197.020 kb on + strand, within Ac3H11_4400 at 197.020 kb on + strand, within Ac3H11_4400 at 197.020 kb on + strand, within Ac3H11_4400 at 197.020 kb on + strand, within Ac3H11_4400 at 197.021 kb on - strand, within Ac3H11_4400 at 197.021 kb on - strand, within Ac3H11_4400 at 197.021 kb on - strand, within Ac3H11_4400 at 197.021 kb on - strand, within Ac3H11_4400 at 197.021 kb on - strand, within Ac3H11_4400 at 197.021 kb on - strand, within Ac3H11_4400 at 197.021 kb on - strand, within Ac3H11_4400 at 197.021 kb on - strand, within Ac3H11_4400 at 197.021 kb on - strand, within Ac3H11_4400 at 197.021 kb on - strand, within Ac3H11_4400 at 197.026 kb on + strand, within Ac3H11_4400 at 197.026 kb on + strand, within Ac3H11_4400 at 197.027 kb on - strand, within Ac3H11_4400 at 197.027 kb on - strand, within Ac3H11_4400 at 197.134 kb on + strand, within Ac3H11_4400 at 197.135 kb on - strand, within Ac3H11_4400 at 197.135 kb on - strand, within Ac3H11_4400 at 197.143 kb on + strand, within Ac3H11_4400 at 197.290 kb on + strand, within Ac3H11_4400 at 197.290 kb on + strand, within Ac3H11_4400 at 197.290 kb on + strand, within Ac3H11_4400 at 197.291 kb on - strand, within Ac3H11_4400 at 197.291 kb on - strand, within Ac3H11_4400 at 197.782 kb on - strand at 197.868 kb on + strand, within Ac3H11_4401 at 197.868 kb on + strand, within Ac3H11_4401 at 197.868 kb on + strand, within Ac3H11_4401 at 197.868 kb on + strand, within Ac3H11_4401 at 197.868 kb on + strand, within Ac3H11_4401 at 197.869 kb on - strand, within Ac3H11_4401 at 197.869 kb on - strand, within Ac3H11_4401 at 197.869 kb on - strand, within Ac3H11_4401 at 197.869 kb on - strand, within Ac3H11_4401 at 197.869 kb on - strand, within Ac3H11_4401 at 197.869 kb on - strand, within Ac3H11_4401 at 198.099 kb on + strand, within Ac3H11_4401 at 198.099 kb on + strand, within Ac3H11_4401 at 198.100 kb on - strand, within Ac3H11_4401 at 198.201 kb on + strand, within Ac3H11_4401 at 198.486 kb on + strand, within Ac3H11_4401 at 198.486 kb on + strand, within Ac3H11_4401 at 198.486 kb on + strand, within Ac3H11_4401 at 198.486 kb on + strand, within Ac3H11_4401 at 198.487 kb on - strand, within Ac3H11_4401 at 198.487 kb on - strand, within Ac3H11_4401 at 198.487 kb on - strand, within Ac3H11_4401 at 198.668 kb on + strand at 198.668 kb on + strand at 198.669 kb on - strand at 198.669 kb on - strand at 198.669 kb on - strand at 198.669 kb on - strand at 198.710 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.005 mg/ml remove 195,947 + Ac3H11_4399 0.24 -0.3 195,947 + Ac3H11_4399 0.24 +0.8 195,948 - Ac3H11_4399 0.24 +1.0 195,948 - Ac3H11_4399 0.24 +2.1 196,076 + Ac3H11_4399 0.38 -1.8 196,128 - Ac3H11_4399 0.43 +0.0 196,214 + Ac3H11_4399 0.52 -1.7 196,214 + Ac3H11_4399 0.52 -3.4 196,215 - Ac3H11_4399 0.52 -2.9 196,215 - Ac3H11_4399 0.52 -1.1 196,215 - Ac3H11_4399 0.52 -2.7 196,215 - Ac3H11_4399 0.52 -1.1 196,349 + Ac3H11_4399 0.65 -2.8 196,349 + Ac3H11_4399 0.65 -0.3 196,349 + Ac3H11_4399 0.65 -2.3 196,349 + Ac3H11_4399 0.65 +0.7 196,349 + Ac3H11_4399 0.65 -0.9 196,349 + Ac3H11_4399 0.65 +1.3 196,350 - Ac3H11_4399 0.66 -2.7 196,350 - Ac3H11_4399 0.66 +1.1 196,350 - Ac3H11_4399 0.66 -1.2 196,350 - Ac3H11_4399 0.66 -2.0 196,415 + Ac3H11_4399 0.72 +0.7 196,416 - Ac3H11_4399 0.72 -0.0 196,416 - Ac3H11_4399 0.72 +1.6 196,552 - Ac3H11_4399 0.86 +1.9 197,020 + Ac3H11_4400 0.21 +1.0 197,020 + Ac3H11_4400 0.21 -1.5 197,020 + Ac3H11_4400 0.21 -2.5 197,020 + Ac3H11_4400 0.21 +1.1 197,020 + Ac3H11_4400 0.21 -1.5 197,020 + Ac3H11_4400 0.21 -4.5 197,020 + Ac3H11_4400 0.21 -4.0 197,020 + Ac3H11_4400 0.21 -1.0 197,020 + Ac3H11_4400 0.21 +2.7 197,020 + Ac3H11_4400 0.21 -0.8 197,020 + Ac3H11_4400 0.21 +1.9 197,020 + Ac3H11_4400 0.21 -2.3 197,020 + Ac3H11_4400 0.21 +0.5 197,021 - Ac3H11_4400 0.21 -0.6 197,021 - Ac3H11_4400 0.21 -2.1 197,021 - Ac3H11_4400 0.21 +0.6 197,021 - Ac3H11_4400 0.21 -1.3 197,021 - Ac3H11_4400 0.21 -3.4 197,021 - Ac3H11_4400 0.21 -2.7 197,021 - Ac3H11_4400 0.21 -1.0 197,021 - Ac3H11_4400 0.21 -1.0 197,021 - Ac3H11_4400 0.21 -0.3 197,021 - Ac3H11_4400 0.21 -0.3 197,026 + Ac3H11_4400 0.22 -1.1 197,026 + Ac3H11_4400 0.22 +0.8 197,027 - Ac3H11_4400 0.22 +0.5 197,027 - Ac3H11_4400 0.22 +1.9 197,134 + Ac3H11_4400 0.34 +1.2 197,135 - Ac3H11_4400 0.34 +0.1 197,135 - Ac3H11_4400 0.34 -0.1 197,143 + Ac3H11_4400 0.35 -0.8 197,290 + Ac3H11_4400 0.51 +0.7 197,290 + Ac3H11_4400 0.51 +2.5 197,290 + Ac3H11_4400 0.51 +1.9 197,291 - Ac3H11_4400 0.51 +1.0 197,291 - Ac3H11_4400 0.51 +0.7 197,782 - +1.9 197,868 + Ac3H11_4401 0.11 -4.4 197,868 + Ac3H11_4401 0.11 -2.7 197,868 + Ac3H11_4401 0.11 -4.9 197,868 + Ac3H11_4401 0.11 -2.6 197,868 + Ac3H11_4401 0.11 -3.7 197,869 - Ac3H11_4401 0.11 -5.2 197,869 - Ac3H11_4401 0.11 -2.9 197,869 - Ac3H11_4401 0.11 -1.5 197,869 - Ac3H11_4401 0.11 -0.3 197,869 - Ac3H11_4401 0.11 -1.5 197,869 - Ac3H11_4401 0.11 -0.9 198,099 + Ac3H11_4401 0.39 -2.5 198,099 + Ac3H11_4401 0.39 -3.3 198,100 - Ac3H11_4401 0.39 -1.1 198,201 + Ac3H11_4401 0.51 -4.1 198,486 + Ac3H11_4401 0.85 -3.5 198,486 + Ac3H11_4401 0.85 -3.2 198,486 + Ac3H11_4401 0.85 -2.0 198,486 + Ac3H11_4401 0.85 -0.9 198,487 - Ac3H11_4401 0.85 -1.7 198,487 - Ac3H11_4401 0.85 -2.0 198,487 - Ac3H11_4401 0.85 -1.7 198,668 + -3.8 198,668 + -1.6 198,669 - +1.6 198,669 - +1.1 198,669 - +0.9 198,669 - +0.6 198,710 + -1.2
Or see this region's nucleotide sequence