Experiment: Cisplatin 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_431 and Ac3H11_432 are separated by 43 nucleotides Ac3H11_432 and Ac3H11_433 are separated by 21 nucleotides Ac3H11_433 and Ac3H11_434 are separated by 99 nucleotides
Ac3H11_431 - Lipid-A-disaccharide synthase (EC 2.4.1.182), at 116,800 to 117,963
_431
Ac3H11_432 - Ribonuclease HII (EC 3.1.26.4), at 118,007 to 118,555
_432
Ac3H11_433 - 23S rRNA (guanosine-2'-O-) -methyltransferase rlmB (EC 2.1.1.-), at 118,577 to 119,350
_433
Ac3H11_434 - hypothetical protein, at 119,450 to 120,211
_434
Position (kb)
118
119
120 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 118.171 kb on + strand, within Ac3H11_432 at 118.171 kb on + strand, within Ac3H11_432 at 118.172 kb on - strand, within Ac3H11_432 at 118.172 kb on - strand, within Ac3H11_432 at 118.434 kb on - strand, within Ac3H11_432 at 118.434 kb on - strand, within Ac3H11_432 at 118.434 kb on - strand, within Ac3H11_432 at 118.508 kb on + strand at 118.508 kb on + strand at 118.508 kb on + strand at 118.508 kb on + strand at 118.572 kb on + strand at 118.572 kb on + strand at 118.572 kb on + strand at 118.573 kb on - strand at 118.788 kb on - strand, within Ac3H11_433 at 119.004 kb on + strand, within Ac3H11_433 at 119.004 kb on + strand, within Ac3H11_433 at 119.005 kb on - strand, within Ac3H11_433 at 119.005 kb on - strand, within Ac3H11_433 at 119.007 kb on + strand, within Ac3H11_433 at 119.007 kb on + strand, within Ac3H11_433 at 119.008 kb on - strand, within Ac3H11_433 at 119.008 kb on - strand, within Ac3H11_433 at 119.162 kb on + strand, within Ac3H11_433 at 119.162 kb on + strand, within Ac3H11_433 at 119.162 kb on + strand, within Ac3H11_433 at 119.163 kb on - strand, within Ac3H11_433 at 119.163 kb on - strand, within Ac3H11_433 at 119.163 kb on - strand, within Ac3H11_433 at 119.163 kb on - strand, within Ac3H11_433 at 119.163 kb on - strand, within Ac3H11_433 at 119.451 kb on + strand at 119.851 kb on - strand, within Ac3H11_434 at 119.851 kb on - strand, within Ac3H11_434 at 119.851 kb on - strand, within Ac3H11_434 at 119.851 kb on - strand, within Ac3H11_434 at 119.937 kb on + strand, within Ac3H11_434 at 119.937 kb on + strand, within Ac3H11_434 at 119.937 kb on + strand, within Ac3H11_434 at 119.937 kb on + strand, within Ac3H11_434 at 119.937 kb on + strand, within Ac3H11_434 at 119.937 kb on + strand, within Ac3H11_434 at 119.937 kb on + strand, within Ac3H11_434 at 119.938 kb on - strand, within Ac3H11_434 at 119.938 kb on - strand, within Ac3H11_434 at 119.938 kb on - strand, within Ac3H11_434 at 119.938 kb on - strand, within Ac3H11_434 at 119.938 kb on - strand, within Ac3H11_434 at 119.938 kb on - strand, within Ac3H11_434 at 119.938 kb on - strand, within Ac3H11_434 at 119.946 kb on + strand, within Ac3H11_434 at 119.946 kb on + strand, within Ac3H11_434 at 119.946 kb on + strand, within Ac3H11_434 at 119.946 kb on + strand, within Ac3H11_434 at 119.946 kb on + strand, within Ac3H11_434 at 119.946 kb on + strand, within Ac3H11_434 at 119.947 kb on - strand, within Ac3H11_434 at 119.947 kb on - strand, within Ac3H11_434 at 119.947 kb on - strand, within Ac3H11_434 at 119.947 kb on - strand, within Ac3H11_434 at 119.947 kb on - strand, within Ac3H11_434 at 119.947 kb on - strand, within Ac3H11_434 at 119.947 kb on - strand, within Ac3H11_434 at 119.947 kb on - strand, within Ac3H11_434 at 119.947 kb on - strand, within Ac3H11_434 at 119.947 kb on - strand, within Ac3H11_434 at 119.965 kb on - strand, within Ac3H11_434 at 119.994 kb on + strand, within Ac3H11_434 at 119.994 kb on + strand, within Ac3H11_434 at 119.994 kb on + strand, within Ac3H11_434 at 119.995 kb on - strand, within Ac3H11_434 at 119.995 kb on - strand, within Ac3H11_434 at 120.021 kb on + strand, within Ac3H11_434 at 120.021 kb on + strand, within Ac3H11_434 at 120.021 kb on + strand, within Ac3H11_434 at 120.021 kb on + strand, within Ac3H11_434 at 120.021 kb on + strand, within Ac3H11_434 at 120.021 kb on + strand, within Ac3H11_434 at 120.021 kb on + strand, within Ac3H11_434 at 120.021 kb on + strand, within Ac3H11_434 at 120.022 kb on - strand, within Ac3H11_434 at 120.022 kb on - strand, within Ac3H11_434 at 120.022 kb on - strand, within Ac3H11_434 at 120.022 kb on - strand, within Ac3H11_434 at 120.022 kb on - strand, within Ac3H11_434 at 120.180 kb on + strand at 120.180 kb on + strand at 120.181 kb on - strand at 120.181 kb on - strand at 120.181 kb on - strand at 120.307 kb on + strand at 120.307 kb on + strand at 120.308 kb on - strand at 120.308 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.005 mg/ml remove 118,171 + Ac3H11_432 0.30 +1.0 118,171 + Ac3H11_432 0.30 +2.2 118,172 - Ac3H11_432 0.30 -2.5 118,172 - Ac3H11_432 0.30 -0.3 118,434 - Ac3H11_432 0.78 +0.0 118,434 - Ac3H11_432 0.78 +0.3 118,434 - Ac3H11_432 0.78 +3.3 118,508 + +1.3 118,508 + +1.3 118,508 + +1.3 118,508 + +2.5 118,572 + +0.9 118,572 + +0.2 118,572 + +0.3 118,573 - +0.6 118,788 - Ac3H11_433 0.27 +0.5 119,004 + Ac3H11_433 0.55 -1.7 119,004 + Ac3H11_433 0.55 +1.6 119,005 - Ac3H11_433 0.55 -0.8 119,005 - Ac3H11_433 0.55 +1.0 119,007 + Ac3H11_433 0.56 +1.2 119,007 + Ac3H11_433 0.56 +2.1 119,008 - Ac3H11_433 0.56 +1.8 119,008 - Ac3H11_433 0.56 -0.4 119,162 + Ac3H11_433 0.76 -0.1 119,162 + Ac3H11_433 0.76 -1.4 119,162 + Ac3H11_433 0.76 +0.3 119,163 - Ac3H11_433 0.76 +0.4 119,163 - Ac3H11_433 0.76 -0.1 119,163 - Ac3H11_433 0.76 -1.8 119,163 - Ac3H11_433 0.76 -2.6 119,163 - Ac3H11_433 0.76 -2.2 119,451 + +1.5 119,851 - Ac3H11_434 0.53 -1.7 119,851 - Ac3H11_434 0.53 +0.8 119,851 - Ac3H11_434 0.53 -1.3 119,851 - Ac3H11_434 0.53 -0.4 119,937 + Ac3H11_434 0.64 -0.0 119,937 + Ac3H11_434 0.64 +0.7 119,937 + Ac3H11_434 0.64 -0.9 119,937 + Ac3H11_434 0.64 -1.5 119,937 + Ac3H11_434 0.64 -0.0 119,937 + Ac3H11_434 0.64 +1.7 119,937 + Ac3H11_434 0.64 -2.8 119,938 - Ac3H11_434 0.64 +1.5 119,938 - Ac3H11_434 0.64 -4.1 119,938 - Ac3H11_434 0.64 -0.3 119,938 - Ac3H11_434 0.64 -3.5 119,938 - Ac3H11_434 0.64 -2.6 119,938 - Ac3H11_434 0.64 +0.4 119,938 - Ac3H11_434 0.64 -0.4 119,946 + Ac3H11_434 0.65 +0.0 119,946 + Ac3H11_434 0.65 -1.6 119,946 + Ac3H11_434 0.65 -2.5 119,946 + Ac3H11_434 0.65 +1.1 119,946 + Ac3H11_434 0.65 +1.0 119,946 + Ac3H11_434 0.65 +1.3 119,947 - Ac3H11_434 0.65 +1.8 119,947 - Ac3H11_434 0.65 +0.6 119,947 - Ac3H11_434 0.65 -0.5 119,947 - Ac3H11_434 0.65 +0.3 119,947 - Ac3H11_434 0.65 -0.0 119,947 - Ac3H11_434 0.65 -2.5 119,947 - Ac3H11_434 0.65 +0.8 119,947 - Ac3H11_434 0.65 -3.0 119,947 - Ac3H11_434 0.65 +0.9 119,947 - Ac3H11_434 0.65 +2.5 119,965 - Ac3H11_434 0.68 +0.3 119,994 + Ac3H11_434 0.71 +3.4 119,994 + Ac3H11_434 0.71 -2.4 119,994 + Ac3H11_434 0.71 +2.0 119,995 - Ac3H11_434 0.72 +0.8 119,995 - Ac3H11_434 0.72 +2.7 120,021 + Ac3H11_434 0.75 -1.0 120,021 + Ac3H11_434 0.75 +0.2 120,021 + Ac3H11_434 0.75 +1.7 120,021 + Ac3H11_434 0.75 -2.8 120,021 + Ac3H11_434 0.75 +0.3 120,021 + Ac3H11_434 0.75 +0.6 120,021 + Ac3H11_434 0.75 -2.5 120,021 + Ac3H11_434 0.75 +0.4 120,022 - Ac3H11_434 0.75 +1.2 120,022 - Ac3H11_434 0.75 +2.2 120,022 - Ac3H11_434 0.75 -2.8 120,022 - Ac3H11_434 0.75 +0.5 120,022 - Ac3H11_434 0.75 -0.2 120,180 + +0.3 120,180 + -0.5 120,181 - -1.2 120,181 - +0.5 120,181 - -2.5 120,307 + +0.6 120,307 + -1.1 120,308 - -1.7 120,308 - -0.4
Or see this region's nucleotide sequence