Experiment: Cisplatin 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_3809 and Ac3H11_3810 are separated by 175 nucleotides Ac3H11_3810 and Ac3H11_3811 are separated by 68 nucleotides Ac3H11_3811 and Ac3H11_3812 are separated by 145 nucleotides
Ac3H11_3809 - tungsten-containing formate dehydrogenase beta subunit, at 23,704 to 25,611
_3809
Ac3H11_3810 - Transcriptional regulator, TetR family, at 25,787 to 26,407
_3810
Ac3H11_3811 - putative membrane protein, at 26,476 to 26,883
_3811
Ac3H11_3812 - Hydrogen peroxide-inducible genes activator, at 27,029 to 27,991
_3812
Position (kb) 26
27 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 25.643 kb on + strand at 25.644 kb on - strand at 25.644 kb on - strand at 25.644 kb on - strand at 25.644 kb on - strand at 25.644 kb on - strand at 25.659 kb on + strand at 25.659 kb on + strand at 25.768 kb on + strand at 25.768 kb on + strand at 25.769 kb on - strand at 25.769 kb on - strand at 25.769 kb on - strand at 26.121 kb on + strand, within Ac3H11_3810 at 26.122 kb on - strand, within Ac3H11_3810 at 26.376 kb on + strand at 26.376 kb on + strand at 26.377 kb on - strand at 26.377 kb on - strand at 26.377 kb on - strand at 26.399 kb on + strand at 26.400 kb on - strand at 26.400 kb on - strand at 26.433 kb on + strand at 26.433 kb on + strand at 26.434 kb on - strand at 26.447 kb on - strand at 26.457 kb on - strand at 26.471 kb on - strand at 26.471 kb on - strand at 26.502 kb on + strand at 26.560 kb on + strand, within Ac3H11_3811 at 26.560 kb on + strand, within Ac3H11_3811 at 26.560 kb on + strand, within Ac3H11_3811 at 26.560 kb on + strand, within Ac3H11_3811 at 26.561 kb on - strand, within Ac3H11_3811 at 26.561 kb on - strand, within Ac3H11_3811 at 26.561 kb on - strand, within Ac3H11_3811 at 26.561 kb on - strand, within Ac3H11_3811 at 26.572 kb on + strand, within Ac3H11_3811 at 26.573 kb on - strand, within Ac3H11_3811 at 26.573 kb on - strand, within Ac3H11_3811 at 26.573 kb on - strand, within Ac3H11_3811 at 26.787 kb on + strand, within Ac3H11_3811 at 26.787 kb on + strand, within Ac3H11_3811 at 26.788 kb on - strand, within Ac3H11_3811 at 26.881 kb on + strand at 26.882 kb on - strand at 26.939 kb on + strand at 26.939 kb on + strand at 26.939 kb on + strand at 26.940 kb on - strand at 26.940 kb on - strand at 26.940 kb on - strand at 26.996 kb on + strand at 27.030 kb on + strand at 27.030 kb on + strand at 27.031 kb on - strand at 27.031 kb on - strand at 27.031 kb on - strand at 27.031 kb on - strand at 27.127 kb on + strand, within Ac3H11_3812 at 27.127 kb on + strand, within Ac3H11_3812 at 27.489 kb on + strand, within Ac3H11_3812 at 27.489 kb on + strand, within Ac3H11_3812 at 27.489 kb on + strand, within Ac3H11_3812 at 27.489 kb on + strand, within Ac3H11_3812 at 27.489 kb on + strand, within Ac3H11_3812 at 27.489 kb on + strand, within Ac3H11_3812 at 27.501 kb on + strand, within Ac3H11_3812 at 27.501 kb on + strand, within Ac3H11_3812 at 27.501 kb on + strand, within Ac3H11_3812 at 27.502 kb on - strand, within Ac3H11_3812 at 27.502 kb on - strand, within Ac3H11_3812 at 27.502 kb on - strand, within Ac3H11_3812 at 27.502 kb on - strand, within Ac3H11_3812 at 27.552 kb on + strand, within Ac3H11_3812 at 27.552 kb on + strand, within Ac3H11_3812 at 27.552 kb on + strand, within Ac3H11_3812
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.005 mg/ml remove 25,643 + -2.2 25,644 - +0.8 25,644 - -0.0 25,644 - -1.7 25,644 - -4.2 25,644 - -2.7 25,659 + +1.6 25,659 + +1.0 25,768 + -1.7 25,768 + +0.4 25,769 - -2.1 25,769 - -2.8 25,769 - -2.3 26,121 + Ac3H11_3810 0.54 -2.4 26,122 - Ac3H11_3810 0.54 -2.1 26,376 + +1.5 26,376 + -0.2 26,377 - -2.9 26,377 - +0.3 26,377 - +2.5 26,399 + -0.3 26,400 - +0.8 26,400 - +0.2 26,433 + -1.2 26,433 + -1.5 26,434 - -0.3 26,447 - -4.1 26,457 - -0.9 26,471 - -1.9 26,471 - -1.5 26,502 + +2.3 26,560 + Ac3H11_3811 0.21 -1.1 26,560 + Ac3H11_3811 0.21 +2.3 26,560 + Ac3H11_3811 0.21 -1.2 26,560 + Ac3H11_3811 0.21 -0.4 26,561 - Ac3H11_3811 0.21 -0.3 26,561 - Ac3H11_3811 0.21 -0.8 26,561 - Ac3H11_3811 0.21 +1.2 26,561 - Ac3H11_3811 0.21 +1.6 26,572 + Ac3H11_3811 0.24 +2.6 26,573 - Ac3H11_3811 0.24 +0.6 26,573 - Ac3H11_3811 0.24 -3.1 26,573 - Ac3H11_3811 0.24 +0.7 26,787 + Ac3H11_3811 0.76 -2.5 26,787 + Ac3H11_3811 0.76 +0.3 26,788 - Ac3H11_3811 0.76 +1.6 26,881 + +0.6 26,882 - +0.6 26,939 + +0.8 26,939 + -2.4 26,939 + +1.4 26,940 - +2.0 26,940 - +0.2 26,940 - -0.2 26,996 + -0.2 27,030 + +0.0 27,030 + -0.9 27,031 - -1.2 27,031 - -1.5 27,031 - +2.2 27,031 - -0.3 27,127 + Ac3H11_3812 0.10 -2.2 27,127 + Ac3H11_3812 0.10 +1.8 27,489 + Ac3H11_3812 0.48 -1.7 27,489 + Ac3H11_3812 0.48 -2.6 27,489 + Ac3H11_3812 0.48 -2.5 27,489 + Ac3H11_3812 0.48 -1.5 27,489 + Ac3H11_3812 0.48 +1.2 27,489 + Ac3H11_3812 0.48 +1.4 27,501 + Ac3H11_3812 0.49 -0.0 27,501 + Ac3H11_3812 0.49 -2.6 27,501 + Ac3H11_3812 0.49 -1.2 27,502 - Ac3H11_3812 0.49 +1.8 27,502 - Ac3H11_3812 0.49 +2.4 27,502 - Ac3H11_3812 0.49 -3.0 27,502 - Ac3H11_3812 0.49 -1.8 27,552 + Ac3H11_3812 0.54 -1.6 27,552 + Ac3H11_3812 0.54 -2.0 27,552 + Ac3H11_3812 0.54 -2.2
Or see this region's nucleotide sequence