Experiment: Cisplatin 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_3517 and Ac3H11_3518 are separated by 110 nucleotides Ac3H11_3518 and Ac3H11_3519 overlap by 17 nucleotides Ac3H11_3519 and Ac3H11_3520 overlap by 16 nucleotides Ac3H11_3520 and Ac3H11_3521 are separated by 31 nucleotides Ac3H11_3521 and Ac3H11_3522 are separated by 131 nucleotides
Ac3H11_3517 - Transcriptional regulator, LysR family, at 62,991 to 63,893
_3517
Ac3H11_3518 - Rrf2-linked NADH-flavin reductase, at 64,004 to 64,645
_3518
Ac3H11_3519 - hypothetical protein, at 64,629 to 64,796
_3519
Ac3H11_3520 - TRAP-type C4-dicarboxylate transport system, large permease component, at 64,781 to 66,061
_3520
Ac3H11_3521 - TRAP-type transport system, small permease component, predicted N-acetylneuraminate transporter, at 66,093 to 66,662
_3521
Ac3H11_3522 - Fumarylacetoacetate hydrolase family protein, at 66,794 to 68,011
_3522
Position (kb) 64
65
66
67 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 63.899 kb on - strand at 63.943 kb on - strand at 63.965 kb on + strand at 63.977 kb on - strand at 64.211 kb on + strand, within Ac3H11_3518 at 64.211 kb on + strand, within Ac3H11_3518 at 64.212 kb on - strand, within Ac3H11_3518 at 64.212 kb on - strand, within Ac3H11_3518 at 64.212 kb on - strand, within Ac3H11_3518 at 64.698 kb on + strand, within Ac3H11_3519 at 64.717 kb on - strand, within Ac3H11_3519 at 64.717 kb on - strand, within Ac3H11_3519 at 64.996 kb on - strand, within Ac3H11_3520 at 65.493 kb on + strand, within Ac3H11_3520 at 65.998 kb on - strand at 66.050 kb on - strand at 66.386 kb on - strand, within Ac3H11_3521 at 66.556 kb on + strand, within Ac3H11_3521 at 66.556 kb on + strand, within Ac3H11_3521 at 66.557 kb on - strand, within Ac3H11_3521 at 66.693 kb on + strand at 66.694 kb on - strand at 66.796 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.005 mg/ml remove 63,899 - +2.4 63,943 - +0.2 63,965 + +1.0 63,977 - -0.2 64,211 + Ac3H11_3518 0.32 +0.4 64,211 + Ac3H11_3518 0.32 -1.9 64,212 - Ac3H11_3518 0.32 -1.4 64,212 - Ac3H11_3518 0.32 -2.3 64,212 - Ac3H11_3518 0.32 -1.0 64,698 + Ac3H11_3519 0.41 +1.3 64,717 - Ac3H11_3519 0.52 +2.3 64,717 - Ac3H11_3519 0.52 -0.4 64,996 - Ac3H11_3520 0.17 +1.5 65,493 + Ac3H11_3520 0.56 +0.8 65,998 - +0.0 66,050 - -0.6 66,386 - Ac3H11_3521 0.51 +0.6 66,556 + Ac3H11_3521 0.81 +1.0 66,556 + Ac3H11_3521 0.81 +1.0 66,557 - Ac3H11_3521 0.81 -3.4 66,693 + -3.9 66,694 - -1.3 66,796 - -0.5
Or see this region's nucleotide sequence