Experiment: Cisplatin 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_3123 and Ac3H11_3124 are separated by 95 nucleotides Ac3H11_3124 and Ac3H11_3125 overlap by 4 nucleotides Ac3H11_3125 and Ac3H11_3126 are separated by 141 nucleotides
Ac3H11_3123 - 50S ribosomal subunit maturation GTPase RbgA (B. subtilis YlqF), at 25,463 to 26,443
_3123
Ac3H11_3124 - hypothetical protein, at 26,539 to 26,790
_3124
Ac3H11_3125 - ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components, at 26,787 to 27,029
_3125
Ac3H11_3126 - Glutamate--cysteine ligase (EC 6.3.2.2), at 27,171 to 28,697
_3126
Position (kb) 26
27
28 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 26.653 kb on + strand, within Ac3H11_3124 at 26.653 kb on + strand, within Ac3H11_3124 at 26.653 kb on + strand, within Ac3H11_3124 at 26.749 kb on + strand, within Ac3H11_3124 at 26.750 kb on - strand, within Ac3H11_3124 at 26.962 kb on + strand, within Ac3H11_3125 at 26.962 kb on + strand, within Ac3H11_3125 at 26.962 kb on + strand, within Ac3H11_3125 at 26.962 kb on + strand, within Ac3H11_3125 at 26.962 kb on + strand, within Ac3H11_3125 at 26.963 kb on - strand, within Ac3H11_3125 at 26.963 kb on - strand, within Ac3H11_3125 at 26.963 kb on - strand, within Ac3H11_3125 at 26.963 kb on - strand, within Ac3H11_3125 at 26.963 kb on - strand, within Ac3H11_3125 at 26.991 kb on + strand, within Ac3H11_3125 at 27.019 kb on + strand at 27.107 kb on + strand at 27.107 kb on + strand at 27.107 kb on + strand at 27.108 kb on - strand at 27.108 kb on - strand at 27.108 kb on - strand at 27.108 kb on - strand at 27.123 kb on + strand at 27.123 kb on + strand at 27.123 kb on + strand at 27.123 kb on + strand at 27.123 kb on + strand at 27.123 kb on + strand at 27.123 kb on + strand at 27.124 kb on - strand at 27.124 kb on - strand at 27.124 kb on - strand at 27.124 kb on - strand at 27.124 kb on - strand at 27.124 kb on - strand at 27.196 kb on + strand at 27.196 kb on + strand at 27.196 kb on + strand at 27.196 kb on + strand at 27.197 kb on - strand at 27.197 kb on - strand at 27.197 kb on - strand at 27.197 kb on - strand at 27.197 kb on - strand at 27.197 kb on - strand at 27.197 kb on - strand at 27.197 kb on - strand at 27.197 kb on - strand at 27.197 kb on - strand at 27.197 kb on - strand at 27.197 kb on - strand at 27.197 kb on - strand at 27.197 kb on - strand at 27.283 kb on + strand at 27.283 kb on + strand at 27.283 kb on + strand at 27.283 kb on + strand at 27.469 kb on + strand, within Ac3H11_3126 at 27.469 kb on + strand, within Ac3H11_3126 at 27.470 kb on - strand, within Ac3H11_3126 at 27.772 kb on + strand, within Ac3H11_3126 at 27.772 kb on + strand, within Ac3H11_3126 at 27.773 kb on - strand, within Ac3H11_3126 at 27.773 kb on - strand, within Ac3H11_3126 at 27.773 kb on - strand, within Ac3H11_3126 at 27.773 kb on - strand, within Ac3H11_3126 at 27.773 kb on - strand, within Ac3H11_3126 at 27.773 kb on - strand, within Ac3H11_3126 at 27.776 kb on - strand, within Ac3H11_3126 at 27.850 kb on + strand, within Ac3H11_3126 at 27.850 kb on + strand, within Ac3H11_3126 at 27.850 kb on + strand, within Ac3H11_3126 at 27.851 kb on - strand, within Ac3H11_3126 at 27.851 kb on - strand, within Ac3H11_3126 at 27.851 kb on - strand, within Ac3H11_3126 at 27.895 kb on + strand, within Ac3H11_3126 at 27.895 kb on + strand, within Ac3H11_3126 at 27.895 kb on + strand, within Ac3H11_3126 at 27.895 kb on + strand, within Ac3H11_3126 at 27.896 kb on - strand, within Ac3H11_3126 at 27.896 kb on - strand, within Ac3H11_3126 at 27.932 kb on - strand, within Ac3H11_3126 at 27.994 kb on + strand, within Ac3H11_3126 at 27.994 kb on + strand, within Ac3H11_3126 at 27.995 kb on - strand, within Ac3H11_3126 at 27.995 kb on - strand, within Ac3H11_3126 at 27.995 kb on - strand, within Ac3H11_3126 at 27.995 kb on - strand, within Ac3H11_3126 at 27.995 kb on - strand, within Ac3H11_3126
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.005 mg/ml remove 26,653 + Ac3H11_3124 0.45 +0.6 26,653 + Ac3H11_3124 0.45 +2.4 26,653 + Ac3H11_3124 0.45 +0.5 26,749 + Ac3H11_3124 0.83 +3.2 26,750 - Ac3H11_3124 0.84 +1.1 26,962 + Ac3H11_3125 0.72 +0.5 26,962 + Ac3H11_3125 0.72 -0.2 26,962 + Ac3H11_3125 0.72 +0.8 26,962 + Ac3H11_3125 0.72 +0.6 26,962 + Ac3H11_3125 0.72 +0.9 26,963 - Ac3H11_3125 0.72 +0.6 26,963 - Ac3H11_3125 0.72 +1.7 26,963 - Ac3H11_3125 0.72 +2.2 26,963 - Ac3H11_3125 0.72 +1.3 26,963 - Ac3H11_3125 0.72 -0.0 26,991 + Ac3H11_3125 0.84 +1.6 27,019 + -1.4 27,107 + -0.7 27,107 + -2.7 27,107 + +0.1 27,108 - +0.1 27,108 - -3.1 27,108 - +1.5 27,108 - +1.7 27,123 + +2.2 27,123 + -0.3 27,123 + -1.0 27,123 + -0.7 27,123 + +0.6 27,123 + +2.4 27,123 + -2.5 27,124 - +0.1 27,124 - +1.0 27,124 - -3.1 27,124 - +1.1 27,124 - +1.3 27,124 - +2.0 27,196 + -0.2 27,196 + -2.6 27,196 + -1.5 27,196 + -3.7 27,197 - -2.2 27,197 - -3.3 27,197 - +1.6 27,197 - -2.4 27,197 - -1.6 27,197 - -0.2 27,197 - -1.1 27,197 - +1.7 27,197 - +2.3 27,197 - -0.7 27,197 - -0.2 27,197 - -3.2 27,197 - +0.4 27,197 - -3.7 27,283 + -1.9 27,283 + -4.1 27,283 + +2.4 27,283 + -2.6 27,469 + Ac3H11_3126 0.20 -2.1 27,469 + Ac3H11_3126 0.20 -2.2 27,470 - Ac3H11_3126 0.20 -0.4 27,772 + Ac3H11_3126 0.39 +1.6 27,772 + Ac3H11_3126 0.39 -0.2 27,773 - Ac3H11_3126 0.39 -2.6 27,773 - Ac3H11_3126 0.39 -1.5 27,773 - Ac3H11_3126 0.39 -1.2 27,773 - Ac3H11_3126 0.39 -1.0 27,773 - Ac3H11_3126 0.39 +2.0 27,773 - Ac3H11_3126 0.39 -1.1 27,776 - Ac3H11_3126 0.40 -1.9 27,850 + Ac3H11_3126 0.44 -3.3 27,850 + Ac3H11_3126 0.44 -0.4 27,850 + Ac3H11_3126 0.44 -0.3 27,851 - Ac3H11_3126 0.45 -3.1 27,851 - Ac3H11_3126 0.45 -1.8 27,851 - Ac3H11_3126 0.45 -1.2 27,895 + Ac3H11_3126 0.47 +0.1 27,895 + Ac3H11_3126 0.47 -2.8 27,895 + Ac3H11_3126 0.47 -1.4 27,895 + Ac3H11_3126 0.47 -1.9 27,896 - Ac3H11_3126 0.47 -2.7 27,896 - Ac3H11_3126 0.47 +0.6 27,932 - Ac3H11_3126 0.50 -0.4 27,994 + Ac3H11_3126 0.54 +1.3 27,994 + Ac3H11_3126 0.54 -1.7 27,995 - Ac3H11_3126 0.54 -2.4 27,995 - Ac3H11_3126 0.54 -1.9 27,995 - Ac3H11_3126 0.54 +0.6 27,995 - Ac3H11_3126 0.54 -2.5 27,995 - Ac3H11_3126 0.54 -0.5
Or see this region's nucleotide sequence