Experiment: Cisplatin 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_183 and Ac3H11_184 are separated by 135 nucleotides Ac3H11_184 and Ac3H11_185 are separated by 105 nucleotides Ac3H11_185 and Ac3H11_186 overlap by 12 nucleotides
Ac3H11_183 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 71,091 to 74,063
_183
Ac3H11_184 - hypothetical protein, at 74,199 to 74,573
_184
Ac3H11_185 - Acetyl-coenzyme A synthetase (EC 6.2.1.1), at 74,679 to 76,364
_185
Ac3H11_186 - hypothetical protein, at 76,353 to 76,529
_186
Position (kb) 74
75
76
77 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 73.679 kb on - strand, within Ac3H11_183 at 73.679 kb on - strand, within Ac3H11_183 at 73.723 kb on + strand, within Ac3H11_183 at 73.723 kb on + strand, within Ac3H11_183 at 73.724 kb on - strand, within Ac3H11_183 at 73.927 kb on + strand at 73.927 kb on + strand at 73.927 kb on + strand at 73.927 kb on + strand at 73.927 kb on + strand at 73.928 kb on - strand at 73.928 kb on - strand at 73.983 kb on + strand at 74.047 kb on + strand at 74.048 kb on - strand at 74.048 kb on - strand at 74.101 kb on + strand at 74.155 kb on + strand at 74.155 kb on + strand at 74.155 kb on + strand at 74.155 kb on + strand at 74.156 kb on - strand at 74.156 kb on - strand at 74.156 kb on - strand at 74.156 kb on - strand at 74.156 kb on - strand at 74.156 kb on - strand at 74.156 kb on - strand at 74.156 kb on - strand at 74.156 kb on - strand at 74.434 kb on + strand, within Ac3H11_184 at 74.434 kb on + strand, within Ac3H11_184 at 74.776 kb on + strand at 74.776 kb on + strand at 74.776 kb on + strand at 74.776 kb on + strand at 74.777 kb on - strand at 74.777 kb on - strand at 74.777 kb on - strand at 74.777 kb on - strand at 74.777 kb on - strand at 74.777 kb on - strand at 74.777 kb on - strand at 74.777 kb on - strand at 74.777 kb on - strand at 75.013 kb on + strand, within Ac3H11_185 at 75.013 kb on + strand, within Ac3H11_185 at 75.113 kb on - strand, within Ac3H11_185 at 75.296 kb on - strand, within Ac3H11_185 at 75.307 kb on + strand, within Ac3H11_185 at 75.308 kb on - strand, within Ac3H11_185 at 75.319 kb on + strand, within Ac3H11_185 at 75.320 kb on - strand, within Ac3H11_185 at 75.379 kb on + strand, within Ac3H11_185 at 75.400 kb on + strand, within Ac3H11_185 at 75.400 kb on + strand, within Ac3H11_185 at 75.400 kb on + strand, within Ac3H11_185 at 75.400 kb on + strand, within Ac3H11_185 at 75.400 kb on + strand, within Ac3H11_185 at 75.400 kb on + strand, within Ac3H11_185 at 75.400 kb on + strand, within Ac3H11_185 at 75.400 kb on + strand, within Ac3H11_185 at 75.400 kb on + strand, within Ac3H11_185 at 75.400 kb on + strand, within Ac3H11_185 at 75.401 kb on - strand, within Ac3H11_185 at 75.401 kb on - strand, within Ac3H11_185 at 75.401 kb on - strand, within Ac3H11_185 at 75.401 kb on - strand, within Ac3H11_185 at 75.401 kb on - strand, within Ac3H11_185 at 75.401 kb on - strand, within Ac3H11_185 at 75.401 kb on - strand, within Ac3H11_185 at 75.401 kb on - strand, within Ac3H11_185 at 75.401 kb on - strand, within Ac3H11_185 at 75.401 kb on - strand, within Ac3H11_185 at 75.401 kb on - strand, within Ac3H11_185 at 75.541 kb on + strand, within Ac3H11_185 at 75.541 kb on + strand, within Ac3H11_185 at 75.541 kb on + strand, within Ac3H11_185 at 75.542 kb on - strand, within Ac3H11_185 at 75.542 kb on - strand, within Ac3H11_185 at 75.542 kb on - strand, within Ac3H11_185 at 75.542 kb on - strand, within Ac3H11_185 at 75.659 kb on + strand, within Ac3H11_185 at 75.715 kb on + strand, within Ac3H11_185 at 75.715 kb on + strand, within Ac3H11_185 at 75.715 kb on + strand, within Ac3H11_185 at 75.715 kb on + strand, within Ac3H11_185 at 75.715 kb on + strand, within Ac3H11_185 at 75.716 kb on - strand, within Ac3H11_185 at 75.716 kb on - strand, within Ac3H11_185 at 76.027 kb on + strand, within Ac3H11_185 at 76.027 kb on + strand, within Ac3H11_185 at 76.027 kb on + strand, within Ac3H11_185 at 76.027 kb on + strand, within Ac3H11_185 at 76.027 kb on + strand, within Ac3H11_185 at 76.028 kb on - strand, within Ac3H11_185 at 76.028 kb on - strand, within Ac3H11_185 at 76.028 kb on - strand, within Ac3H11_185 at 76.082 kb on + strand, within Ac3H11_185 at 76.082 kb on + strand, within Ac3H11_185 at 76.083 kb on - strand, within Ac3H11_185 at 76.083 kb on - strand, within Ac3H11_185 at 76.219 kb on + strand at 76.219 kb on + strand at 76.219 kb on + strand at 76.219 kb on + strand at 76.220 kb on - strand at 76.220 kb on - strand at 76.220 kb on - strand at 76.220 kb on - strand at 76.364 kb on + strand at 76.365 kb on - strand at 76.365 kb on - strand at 76.397 kb on - strand, within Ac3H11_186 at 76.510 kb on + strand, within Ac3H11_186 at 76.510 kb on + strand, within Ac3H11_186 at 76.510 kb on + strand, within Ac3H11_186 at 76.510 kb on + strand, within Ac3H11_186 at 76.511 kb on - strand, within Ac3H11_186 at 76.511 kb on - strand, within Ac3H11_186 at 76.511 kb on - strand, within Ac3H11_186 at 76.512 kb on - strand at 76.635 kb on + strand at 76.635 kb on + strand at 76.635 kb on + strand at 76.635 kb on + strand at 76.636 kb on - strand at 76.636 kb on - strand at 76.638 kb on + strand at 76.639 kb on - strand at 76.744 kb on + strand at 76.744 kb on + strand at 76.745 kb on - strand at 76.745 kb on - strand at 76.824 kb on - strand at 76.884 kb on + strand at 76.885 kb on - strand at 76.980 kb on - strand at 77.013 kb on + strand at 77.013 kb on + strand at 77.072 kb on - strand at 77.072 kb on - strand at 77.079 kb on + strand at 77.079 kb on + strand at 77.080 kb on - strand at 77.114 kb on + strand at 77.114 kb on + strand at 77.115 kb on - strand at 77.115 kb on - strand at 77.115 kb on - strand at 77.182 kb on + strand at 77.182 kb on + strand at 77.182 kb on + strand at 77.182 kb on + strand at 77.182 kb on + strand at 77.182 kb on + strand at 77.182 kb on + strand at 77.182 kb on + strand at 77.183 kb on - strand at 77.183 kb on - strand at 77.183 kb on - strand at 77.183 kb on - strand at 77.183 kb on - strand at 77.183 kb on - strand at 77.184 kb on + strand at 77.184 kb on + strand at 77.184 kb on + strand at 77.184 kb on + strand at 77.184 kb on + strand at 77.184 kb on + strand at 77.184 kb on + strand at 77.184 kb on + strand at 77.185 kb on - strand at 77.185 kb on - strand at 77.185 kb on - strand at 77.190 kb on + strand at 77.190 kb on + strand at 77.279 kb on + strand at 77.279 kb on + strand at 77.279 kb on + strand at 77.279 kb on + strand at 77.279 kb on + strand at 77.279 kb on + strand at 77.279 kb on + strand at 77.279 kb on + strand at 77.279 kb on + strand at 77.279 kb on + strand at 77.280 kb on - strand at 77.280 kb on - strand at 77.280 kb on - strand at 77.280 kb on - strand at 77.280 kb on - strand at 77.280 kb on - strand at 77.280 kb on - strand at 77.280 kb on - strand at 77.280 kb on - strand at 77.280 kb on - strand at 77.280 kb on - strand at 77.280 kb on - strand at 77.280 kb on - strand at 77.280 kb on - strand at 77.280 kb on - strand at 77.280 kb on - strand at 77.280 kb on - strand at 77.280 kb on - strand at 77.313 kb on + strand at 77.314 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.005 mg/ml remove 73,679 - Ac3H11_183 0.87 +2.7 73,679 - Ac3H11_183 0.87 -1.0 73,723 + Ac3H11_183 0.89 +0.9 73,723 + Ac3H11_183 0.89 -2.0 73,724 - Ac3H11_183 0.89 +0.3 73,927 + -0.8 73,927 + +0.3 73,927 + -0.5 73,927 + +1.0 73,927 + +1.0 73,928 - -2.0 73,928 - +0.7 73,983 + -1.1 74,047 + +0.5 74,048 - -2.3 74,048 - +1.3 74,101 + -0.9 74,155 + -1.1 74,155 + -0.1 74,155 + -1.4 74,155 + -0.2 74,156 - -0.0 74,156 - -0.7 74,156 - +2.7 74,156 - +1.5 74,156 - +1.9 74,156 - +0.9 74,156 - -1.8 74,156 - +0.7 74,156 - -0.8 74,434 + Ac3H11_184 0.63 +0.0 74,434 + Ac3H11_184 0.63 -3.5 74,776 + +1.6 74,776 + +0.4 74,776 + +0.7 74,776 + -0.4 74,777 - -2.3 74,777 - +0.4 74,777 - -0.8 74,777 - +1.2 74,777 - +0.8 74,777 - +0.4 74,777 - -1.3 74,777 - +2.2 74,777 - -0.7 75,013 + Ac3H11_185 0.20 +1.9 75,013 + Ac3H11_185 0.20 -0.2 75,113 - Ac3H11_185 0.26 -1.0 75,296 - Ac3H11_185 0.37 -2.7 75,307 + Ac3H11_185 0.37 -0.4 75,308 - Ac3H11_185 0.37 -0.2 75,319 + Ac3H11_185 0.38 +1.3 75,320 - Ac3H11_185 0.38 +0.7 75,379 + Ac3H11_185 0.42 +0.0 75,400 + Ac3H11_185 0.43 -1.9 75,400 + Ac3H11_185 0.43 +1.0 75,400 + Ac3H11_185 0.43 -2.3 75,400 + Ac3H11_185 0.43 -1.0 75,400 + Ac3H11_185 0.43 -1.1 75,400 + Ac3H11_185 0.43 +0.1 75,400 + Ac3H11_185 0.43 +0.0 75,400 + Ac3H11_185 0.43 -0.6 75,400 + Ac3H11_185 0.43 +0.6 75,400 + Ac3H11_185 0.43 +0.1 75,401 - Ac3H11_185 0.43 +1.3 75,401 - Ac3H11_185 0.43 +0.3 75,401 - Ac3H11_185 0.43 +1.7 75,401 - Ac3H11_185 0.43 +1.4 75,401 - Ac3H11_185 0.43 -1.6 75,401 - Ac3H11_185 0.43 +0.4 75,401 - Ac3H11_185 0.43 -0.4 75,401 - Ac3H11_185 0.43 +2.7 75,401 - Ac3H11_185 0.43 +1.0 75,401 - Ac3H11_185 0.43 +2.2 75,401 - Ac3H11_185 0.43 +0.6 75,541 + Ac3H11_185 0.51 -0.5 75,541 + Ac3H11_185 0.51 -2.4 75,541 + Ac3H11_185 0.51 +0.3 75,542 - Ac3H11_185 0.51 +0.8 75,542 - Ac3H11_185 0.51 +1.0 75,542 - Ac3H11_185 0.51 +1.1 75,542 - Ac3H11_185 0.51 -0.1 75,659 + Ac3H11_185 0.58 +0.7 75,715 + Ac3H11_185 0.61 -0.8 75,715 + Ac3H11_185 0.61 +3.4 75,715 + Ac3H11_185 0.61 +0.1 75,715 + Ac3H11_185 0.61 +1.0 75,715 + Ac3H11_185 0.61 +1.6 75,716 - Ac3H11_185 0.62 -0.3 75,716 - Ac3H11_185 0.62 -1.1 76,027 + Ac3H11_185 0.80 +0.6 76,027 + Ac3H11_185 0.80 +0.6 76,027 + Ac3H11_185 0.80 -2.3 76,027 + Ac3H11_185 0.80 +0.6 76,027 + Ac3H11_185 0.80 -3.3 76,028 - Ac3H11_185 0.80 +1.0 76,028 - Ac3H11_185 0.80 -1.3 76,028 - Ac3H11_185 0.80 -0.2 76,082 + Ac3H11_185 0.83 +2.8 76,082 + Ac3H11_185 0.83 +1.4 76,083 - Ac3H11_185 0.83 -1.9 76,083 - Ac3H11_185 0.83 -3.1 76,219 + -1.1 76,219 + +1.9 76,219 + +0.3 76,219 + -1.1 76,220 - +0.6 76,220 - +0.8 76,220 - +0.1 76,220 - +1.5 76,364 + -0.0 76,365 - -2.8 76,365 - +0.3 76,397 - Ac3H11_186 0.25 +0.4 76,510 + Ac3H11_186 0.89 +0.6 76,510 + Ac3H11_186 0.89 -3.4 76,510 + Ac3H11_186 0.89 +1.5 76,510 + Ac3H11_186 0.89 +2.2 76,511 - Ac3H11_186 0.89 +2.2 76,511 - Ac3H11_186 0.89 +0.2 76,511 - Ac3H11_186 0.89 +0.8 76,512 - +0.4 76,635 + +1.1 76,635 + +0.7 76,635 + +0.7 76,635 + -0.1 76,636 - -0.9 76,636 - -1.9 76,638 + +1.2 76,639 - -1.2 76,744 + +0.3 76,744 + -1.0 76,745 - +0.1 76,745 - -1.6 76,824 - +0.7 76,884 + +0.0 76,885 - -0.2 76,980 - -0.8 77,013 + -1.7 77,013 + -1.1 77,072 - +1.7 77,072 - +0.7 77,079 + +1.1 77,079 + -0.5 77,080 - -0.3 77,114 + +1.4 77,114 + -1.4 77,115 - +0.6 77,115 - +0.9 77,115 - +0.5 77,182 + -1.4 77,182 + +0.6 77,182 + +1.1 77,182 + +0.1 77,182 + -0.1 77,182 + +0.5 77,182 + +1.1 77,182 + -2.2 77,183 - -0.2 77,183 - +2.7 77,183 - +1.4 77,183 - +0.7 77,183 - +0.7 77,183 - +0.6 77,184 + +0.5 77,184 + +1.1 77,184 + +2.3 77,184 + +0.5 77,184 + +0.6 77,184 + +1.5 77,184 + +0.3 77,184 + +2.0 77,185 - -2.9 77,185 - +1.9 77,185 - +0.3 77,190 + +2.3 77,190 + +0.3 77,279 + +0.8 77,279 + -1.2 77,279 + +1.4 77,279 + -1.2 77,279 + +0.8 77,279 + +0.3 77,279 + +0.9 77,279 + -2.0 77,279 + +2.5 77,279 + +1.2 77,280 - -1.4 77,280 - -2.8 77,280 - -3.2 77,280 - -1.4 77,280 - +1.9 77,280 - -0.0 77,280 - +0.6 77,280 - +0.2 77,280 - +0.3 77,280 - -0.5 77,280 - +1.6 77,280 - +0.6 77,280 - -0.4 77,280 - +0.6 77,280 - -1.7 77,280 - -2.0 77,280 - +3.1 77,280 - -3.5 77,313 + -2.0 77,314 - +1.9
Or see this region's nucleotide sequence