Experiment: Cisplatin 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_1763 and Ac3H11_1764 are separated by 23 nucleotides Ac3H11_1764 and Ac3H11_1765 overlap by 1 nucleotides
Ac3H11_1763 - Adenylate cyclase (EC 4.6.1.1), at 375,072 to 377,384
_1763
Ac3H11_1764 - Selenophosphate-dependent tRNA 2-selenouridine synthase, at 377,408 to 378,553
_1764
Ac3H11_1765 - Selenide,water dikinase (EC 2.7.9.3), at 378,553 to 379,605
_1765
Position (kb)
377
378
379 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 377.383 kb on - strand at 377.383 kb on - strand at 377.383 kb on - strand at 377.383 kb on - strand at 377.541 kb on + strand, within Ac3H11_1764 at 377.541 kb on + strand, within Ac3H11_1764 at 377.542 kb on - strand, within Ac3H11_1764 at 377.547 kb on + strand, within Ac3H11_1764 at 377.547 kb on + strand, within Ac3H11_1764 at 377.547 kb on + strand, within Ac3H11_1764 at 377.547 kb on + strand, within Ac3H11_1764 at 377.559 kb on + strand, within Ac3H11_1764 at 377.559 kb on + strand, within Ac3H11_1764 at 377.559 kb on + strand, within Ac3H11_1764 at 377.560 kb on - strand, within Ac3H11_1764 at 377.560 kb on - strand, within Ac3H11_1764 at 377.560 kb on - strand, within Ac3H11_1764 at 377.560 kb on - strand, within Ac3H11_1764 at 377.562 kb on + strand, within Ac3H11_1764 at 377.562 kb on + strand, within Ac3H11_1764 at 377.562 kb on + strand, within Ac3H11_1764 at 377.563 kb on - strand, within Ac3H11_1764 at 377.563 kb on - strand, within Ac3H11_1764 at 377.787 kb on + strand, within Ac3H11_1764 at 377.787 kb on + strand, within Ac3H11_1764 at 377.787 kb on + strand, within Ac3H11_1764 at 377.787 kb on + strand, within Ac3H11_1764 at 377.787 kb on + strand, within Ac3H11_1764 at 377.787 kb on + strand, within Ac3H11_1764 at 377.788 kb on - strand, within Ac3H11_1764 at 377.788 kb on - strand, within Ac3H11_1764 at 377.788 kb on - strand, within Ac3H11_1764 at 377.788 kb on - strand at 378.183 kb on + strand, within Ac3H11_1764 at 378.184 kb on - strand, within Ac3H11_1764 at 378.204 kb on + strand, within Ac3H11_1764 at 378.273 kb on + strand, within Ac3H11_1764 at 378.273 kb on + strand, within Ac3H11_1764 at 378.396 kb on + strand, within Ac3H11_1764 at 378.397 kb on - strand, within Ac3H11_1764 at 378.397 kb on - strand, within Ac3H11_1764 at 378.397 kb on - strand, within Ac3H11_1764 at 378.749 kb on + strand, within Ac3H11_1765 at 378.749 kb on + strand, within Ac3H11_1765 at 378.749 kb on + strand, within Ac3H11_1765 at 378.750 kb on - strand, within Ac3H11_1765 at 378.750 kb on - strand, within Ac3H11_1765 at 378.800 kb on + strand, within Ac3H11_1765 at 378.800 kb on + strand, within Ac3H11_1765 at 378.800 kb on + strand, within Ac3H11_1765 at 378.800 kb on + strand, within Ac3H11_1765 at 378.800 kb on + strand, within Ac3H11_1765 at 378.800 kb on + strand, within Ac3H11_1765 at 378.800 kb on + strand, within Ac3H11_1765 at 378.800 kb on + strand, within Ac3H11_1765 at 378.800 kb on + strand, within Ac3H11_1765 at 378.800 kb on + strand, within Ac3H11_1765 at 378.801 kb on - strand, within Ac3H11_1765 at 378.801 kb on - strand, within Ac3H11_1765 at 378.801 kb on - strand, within Ac3H11_1765 at 378.801 kb on - strand, within Ac3H11_1765 at 378.801 kb on - strand, within Ac3H11_1765 at 379.068 kb on - strand, within Ac3H11_1765 at 379.313 kb on + strand, within Ac3H11_1765 at 379.313 kb on + strand, within Ac3H11_1765 at 379.313 kb on + strand, within Ac3H11_1765 at 379.314 kb on - strand, within Ac3H11_1765 at 379.314 kb on - strand, within Ac3H11_1765 at 379.314 kb on - strand, within Ac3H11_1765 at 379.314 kb on - strand, within Ac3H11_1765 at 379.314 kb on - strand, within Ac3H11_1765 at 379.427 kb on + strand, within Ac3H11_1765 at 379.428 kb on - strand, within Ac3H11_1765 at 379.428 kb on - strand, within Ac3H11_1765 at 379.428 kb on - strand, within Ac3H11_1765
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.005 mg/ml remove 377,383 - +1.0 377,383 - -1.5 377,383 - -0.6 377,383 - +0.6 377,541 + Ac3H11_1764 0.12 -0.8 377,541 + Ac3H11_1764 0.12 -2.2 377,542 - Ac3H11_1764 0.12 +3.1 377,547 + Ac3H11_1764 0.12 -3.4 377,547 + Ac3H11_1764 0.12 -1.1 377,547 + Ac3H11_1764 0.12 +2.8 377,547 + Ac3H11_1764 0.12 +0.2 377,559 + Ac3H11_1764 0.13 +2.0 377,559 + Ac3H11_1764 0.13 +1.1 377,559 + Ac3H11_1764 0.13 -2.0 377,560 - Ac3H11_1764 0.13 +0.6 377,560 - Ac3H11_1764 0.13 +1.3 377,560 - Ac3H11_1764 0.13 +0.0 377,560 - Ac3H11_1764 0.13 -0.2 377,562 + Ac3H11_1764 0.13 +2.9 377,562 + Ac3H11_1764 0.13 -1.3 377,562 + Ac3H11_1764 0.13 -1.0 377,563 - Ac3H11_1764 0.14 +1.0 377,563 - Ac3H11_1764 0.14 +1.1 377,787 + Ac3H11_1764 0.33 +0.7 377,787 + Ac3H11_1764 0.33 -2.2 377,787 + Ac3H11_1764 0.33 +0.3 377,787 + Ac3H11_1764 0.33 +1.1 377,787 + Ac3H11_1764 0.33 +0.3 377,787 + Ac3H11_1764 0.33 +0.2 377,788 - Ac3H11_1764 0.33 -0.9 377,788 - Ac3H11_1764 0.33 +0.5 377,788 - Ac3H11_1764 0.33 +0.4 377,788 - +0.0 378,183 + Ac3H11_1764 0.68 -2.3 378,184 - Ac3H11_1764 0.68 -1.0 378,204 + Ac3H11_1764 0.69 +1.1 378,273 + Ac3H11_1764 0.75 -2.5 378,273 + Ac3H11_1764 0.75 +0.4 378,396 + Ac3H11_1764 0.86 +0.2 378,397 - Ac3H11_1764 0.86 -2.3 378,397 - Ac3H11_1764 0.86 +3.4 378,397 - Ac3H11_1764 0.86 -3.1 378,749 + Ac3H11_1765 0.19 +2.0 378,749 + Ac3H11_1765 0.19 -0.2 378,749 + Ac3H11_1765 0.19 +0.8 378,750 - Ac3H11_1765 0.19 +1.5 378,750 - Ac3H11_1765 0.19 +1.6 378,800 + Ac3H11_1765 0.23 -0.0 378,800 + Ac3H11_1765 0.23 +1.4 378,800 + Ac3H11_1765 0.23 -0.7 378,800 + Ac3H11_1765 0.23 +1.6 378,800 + Ac3H11_1765 0.23 -0.3 378,800 + Ac3H11_1765 0.23 +1.9 378,800 + Ac3H11_1765 0.23 -1.0 378,800 + Ac3H11_1765 0.23 -1.2 378,800 + Ac3H11_1765 0.23 -2.5 378,800 + Ac3H11_1765 0.23 -4.1 378,801 - Ac3H11_1765 0.24 -1.5 378,801 - Ac3H11_1765 0.24 -3.5 378,801 - Ac3H11_1765 0.24 -2.5 378,801 - Ac3H11_1765 0.24 +2.2 378,801 - Ac3H11_1765 0.24 -0.6 379,068 - Ac3H11_1765 0.49 -1.5 379,313 + Ac3H11_1765 0.72 +0.6 379,313 + Ac3H11_1765 0.72 +1.2 379,313 + Ac3H11_1765 0.72 +5.0 379,314 - Ac3H11_1765 0.72 +1.4 379,314 - Ac3H11_1765 0.72 +1.7 379,314 - Ac3H11_1765 0.72 +1.1 379,314 - Ac3H11_1765 0.72 -0.4 379,314 - Ac3H11_1765 0.72 -0.0 379,427 + Ac3H11_1765 0.83 -3.1 379,428 - Ac3H11_1765 0.83 -3.3 379,428 - Ac3H11_1765 0.83 -1.1 379,428 - Ac3H11_1765 0.83 +1.0
Or see this region's nucleotide sequence