Experiment: Cisplatin 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_1299 and Ac3H11_1300 are separated by 70 nucleotides Ac3H11_1300 and Ac3H11_1301 are separated by 265 nucleotides Ac3H11_1301 and Ac3H11_1302 are separated by 223 nucleotides
Ac3H11_1299 - PhnB protein; putative DNA binding 3-demethylubiquinone-9 3-methyltransferase domain protein, at 114,339 to 114,767
_1299
Ac3H11_1300 - hypothetical protein, at 114,838 to 115,323
_1300
Ac3H11_1301 - MFS family multidrug efflux protein in Burkholderiaceae, unknown substrate, at 115,589 to 117,070
_1301
Ac3H11_1302 - Pirin, N-terminal:Pirin, C-terminal, at 117,294 to 118,391
_1302
Position (kb)
115
116
117
118 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 115.120 kb on + strand, within Ac3H11_1300 at 115.121 kb on - strand, within Ac3H11_1300 at 115.306 kb on + strand at 115.306 kb on + strand at 115.307 kb on - strand at 115.307 kb on - strand at 115.307 kb on - strand at 115.397 kb on + strand at 115.426 kb on + strand at 115.426 kb on + strand at 115.427 kb on - strand at 115.796 kb on + strand, within Ac3H11_1301 at 115.796 kb on + strand, within Ac3H11_1301 at 115.796 kb on + strand, within Ac3H11_1301 at 115.796 kb on + strand, within Ac3H11_1301 at 115.796 kb on + strand, within Ac3H11_1301 at 115.796 kb on + strand, within Ac3H11_1301 at 115.796 kb on + strand, within Ac3H11_1301 at 115.796 kb on + strand, within Ac3H11_1301 at 115.797 kb on - strand, within Ac3H11_1301 at 115.797 kb on - strand, within Ac3H11_1301 at 115.797 kb on - strand, within Ac3H11_1301 at 115.797 kb on - strand, within Ac3H11_1301 at 115.797 kb on - strand, within Ac3H11_1301 at 115.797 kb on - strand, within Ac3H11_1301 at 115.797 kb on - strand, within Ac3H11_1301 at 115.797 kb on - strand, within Ac3H11_1301 at 115.850 kb on + strand, within Ac3H11_1301 at 115.850 kb on + strand, within Ac3H11_1301 at 115.851 kb on - strand, within Ac3H11_1301 at 116.054 kb on + strand, within Ac3H11_1301 at 116.054 kb on + strand, within Ac3H11_1301 at 116.054 kb on + strand, within Ac3H11_1301 at 116.055 kb on - strand, within Ac3H11_1301 at 116.055 kb on - strand, within Ac3H11_1301 at 116.055 kb on - strand, within Ac3H11_1301 at 116.055 kb on - strand, within Ac3H11_1301 at 116.055 kb on - strand, within Ac3H11_1301 at 116.171 kb on + strand, within Ac3H11_1301 at 116.171 kb on + strand, within Ac3H11_1301 at 116.172 kb on - strand, within Ac3H11_1301 at 116.279 kb on + strand, within Ac3H11_1301 at 116.279 kb on + strand, within Ac3H11_1301 at 116.279 kb on + strand, within Ac3H11_1301 at 116.279 kb on + strand, within Ac3H11_1301 at 116.280 kb on - strand, within Ac3H11_1301 at 116.280 kb on - strand, within Ac3H11_1301 at 116.280 kb on - strand, within Ac3H11_1301 at 116.280 kb on - strand, within Ac3H11_1301 at 116.280 kb on - strand, within Ac3H11_1301 at 116.280 kb on - strand, within Ac3H11_1301 at 116.504 kb on + strand, within Ac3H11_1301 at 116.504 kb on + strand, within Ac3H11_1301 at 116.504 kb on + strand, within Ac3H11_1301 at 116.505 kb on - strand, within Ac3H11_1301 at 116.505 kb on - strand, within Ac3H11_1301 at 116.505 kb on - strand, within Ac3H11_1301 at 116.505 kb on - strand, within Ac3H11_1301 at 116.525 kb on + strand, within Ac3H11_1301 at 116.765 kb on - strand, within Ac3H11_1301 at 116.796 kb on + strand, within Ac3H11_1301 at 116.796 kb on + strand, within Ac3H11_1301 at 116.796 kb on + strand, within Ac3H11_1301 at 116.797 kb on - strand, within Ac3H11_1301 at 117.085 kb on + strand at 117.086 kb on - strand at 117.241 kb on - strand at 117.504 kb on + strand, within Ac3H11_1302 at 117.504 kb on + strand, within Ac3H11_1302 at 117.504 kb on + strand, within Ac3H11_1302 at 117.505 kb on - strand, within Ac3H11_1302 at 118.035 kb on + strand, within Ac3H11_1302 at 118.035 kb on + strand, within Ac3H11_1302 at 118.035 kb on + strand, within Ac3H11_1302 at 118.035 kb on + strand, within Ac3H11_1302 at 118.035 kb on + strand, within Ac3H11_1302 at 118.036 kb on - strand, within Ac3H11_1302
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.005 mg/ml remove 115,120 + Ac3H11_1300 0.58 +2.4 115,121 - Ac3H11_1300 0.58 -1.0 115,306 + -1.5 115,306 + -1.0 115,307 - -1.9 115,307 - +0.2 115,307 - -0.7 115,397 + -2.5 115,426 + -3.8 115,426 + -3.4 115,427 - -1.7 115,796 + Ac3H11_1301 0.14 -2.5 115,796 + Ac3H11_1301 0.14 -0.7 115,796 + Ac3H11_1301 0.14 +1.4 115,796 + Ac3H11_1301 0.14 -0.0 115,796 + Ac3H11_1301 0.14 -2.8 115,796 + Ac3H11_1301 0.14 +3.1 115,796 + Ac3H11_1301 0.14 +0.4 115,796 + Ac3H11_1301 0.14 +0.2 115,797 - Ac3H11_1301 0.14 +3.0 115,797 - Ac3H11_1301 0.14 -2.3 115,797 - Ac3H11_1301 0.14 +2.7 115,797 - Ac3H11_1301 0.14 -1.0 115,797 - Ac3H11_1301 0.14 -2.9 115,797 - Ac3H11_1301 0.14 -1.1 115,797 - Ac3H11_1301 0.14 +0.1 115,797 - Ac3H11_1301 0.14 +1.3 115,850 + Ac3H11_1301 0.18 +2.9 115,850 + Ac3H11_1301 0.18 +1.0 115,851 - Ac3H11_1301 0.18 -3.2 116,054 + Ac3H11_1301 0.31 -0.6 116,054 + Ac3H11_1301 0.31 +0.2 116,054 + Ac3H11_1301 0.31 -1.6 116,055 - Ac3H11_1301 0.31 +1.7 116,055 - Ac3H11_1301 0.31 +1.4 116,055 - Ac3H11_1301 0.31 -0.4 116,055 - Ac3H11_1301 0.31 +0.6 116,055 - Ac3H11_1301 0.31 -1.9 116,171 + Ac3H11_1301 0.39 -1.7 116,171 + Ac3H11_1301 0.39 -2.6 116,172 - Ac3H11_1301 0.39 -0.6 116,279 + Ac3H11_1301 0.47 -2.8 116,279 + Ac3H11_1301 0.47 -0.6 116,279 + Ac3H11_1301 0.47 -0.9 116,279 + Ac3H11_1301 0.47 +1.4 116,280 - Ac3H11_1301 0.47 +1.1 116,280 - Ac3H11_1301 0.47 +1.4 116,280 - Ac3H11_1301 0.47 +0.2 116,280 - Ac3H11_1301 0.47 +0.1 116,280 - Ac3H11_1301 0.47 -0.2 116,280 - Ac3H11_1301 0.47 -1.7 116,504 + Ac3H11_1301 0.62 -2.3 116,504 + Ac3H11_1301 0.62 +1.1 116,504 + Ac3H11_1301 0.62 -3.4 116,505 - Ac3H11_1301 0.62 +3.5 116,505 - Ac3H11_1301 0.62 -2.3 116,505 - Ac3H11_1301 0.62 -0.7 116,505 - Ac3H11_1301 0.62 -1.0 116,525 + Ac3H11_1301 0.63 +1.5 116,765 - Ac3H11_1301 0.79 +2.8 116,796 + Ac3H11_1301 0.81 +2.2 116,796 + Ac3H11_1301 0.81 -2.5 116,796 + Ac3H11_1301 0.81 +2.2 116,797 - Ac3H11_1301 0.82 +0.2 117,085 + +0.1 117,086 - +1.0 117,241 - +1.0 117,504 + Ac3H11_1302 0.19 +0.7 117,504 + Ac3H11_1302 0.19 +1.2 117,504 + Ac3H11_1302 0.19 +0.2 117,505 - Ac3H11_1302 0.19 -0.4 118,035 + Ac3H11_1302 0.67 -2.6 118,035 + Ac3H11_1302 0.67 -0.3 118,035 + Ac3H11_1302 0.67 +0.9 118,035 + Ac3H11_1302 0.67 -2.2 118,035 + Ac3H11_1302 0.67 +1.1 118,036 - Ac3H11_1302 0.68 +0.6
Or see this region's nucleotide sequence