Experiment: Glycine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt engB and Shewana3_4111 are separated by 169 nucleotides Shewana3_4111 and Shewana3_4112 are separated by 108 nucleotides Shewana3_4112 and Shewana3_4113 are separated by 421 nucleotides
Shewana3_4110: engB - ribosome biogenesis GTP-binding protein YsxC (RefSeq), at 4,935,061 to 4,935,720
engB
Shewana3_4111: Shewana3_4111 - cytochrome c, class I (RefSeq), at 4,935,890 to 4,936,513
_4111
Shewana3_4112: Shewana3_4112 - hypothetical protein (RefSeq), at 4,936,622 to 4,936,915
_4112
Shewana3_4113: Shewana3_4113 - hypothetical protein (RefSeq), at 4,937,337 to 4,937,975
_4113
Position (kb) 4935
4936
4937 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4935.108 kb on + strand at 4935.108 kb on - strand at 4935.805 kb on - strand at 4935.844 kb on + strand at 4935.853 kb on + strand at 4935.858 kb on + strand at 4935.942 kb on - strand at 4936.568 kb on + strand at 4936.637 kb on - strand at 4937.038 kb on + strand at 4937.256 kb on - strand at 4937.299 kb on - strand at 4937.352 kb on - strand at 4937.439 kb on - strand, within Shewana3_4113
Per-strain Table
Position Strand Gene LocusTag Fraction Glycine (N) remove 4,935,108 + +2.2 4,935,108 - +1.0 4,935,805 - -1.4 4,935,844 + -1.6 4,935,853 + -2.9 4,935,858 + -1.5 4,935,942 - -1.4 4,936,568 + +0.7 4,936,637 - +0.3 4,937,038 + +1.9 4,937,256 - +1.9 4,937,299 - -1.7 4,937,352 - +0.8 4,937,439 - Shewana3_4113 0.16 -0.4
Or see this region's nucleotide sequence