Experiment: Glycine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rimM and rpsP are separated by 20 nucleotides rpsP and Shewana3_3018 are separated by 218 nucleotides Shewana3_3018 and Shewana3_3019 are separated by 288 nucleotides
Shewana3_3016: rimM - 16S rRNA-processing protein RimM (RefSeq), at 3,622,837 to 3,623,367
rimM
Shewana3_3017: rpsP - 30S ribosomal protein S16 (RefSeq), at 3,623,388 to 3,623,636
rpsP
Shewana3_3018: Shewana3_3018 - signal recognition particle protein (RefSeq), at 3,623,855 to 3,625,228
_3018
Shewana3_3019: Shewana3_3019 - cytochrome c assembly protein (RefSeq), at 3,625,517 to 3,626,305
_3019
Position (kb)
3623
3624
3625
3626 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3625.361 kb on + strand at 3625.486 kb on + strand at 3625.585 kb on + strand at 3625.796 kb on + strand, within Shewana3_3019 at 3625.818 kb on + strand, within Shewana3_3019 at 3625.892 kb on + strand, within Shewana3_3019 at 3625.902 kb on + strand at 3625.932 kb on - strand, within Shewana3_3019 at 3626.191 kb on + strand, within Shewana3_3019 at 3626.191 kb on + strand, within Shewana3_3019
Per-strain Table
Position Strand Gene LocusTag Fraction Glycine (N) remove 3,625,361 + -1.0 3,625,486 + -3.2 3,625,585 + -2.8 3,625,796 + Shewana3_3019 0.35 -5.7 3,625,818 + Shewana3_3019 0.38 -3.3 3,625,892 + Shewana3_3019 0.48 -3.0 3,625,902 + -0.6 3,625,932 - Shewana3_3019 0.53 -4.2 3,626,191 + Shewana3_3019 0.85 -3.1 3,626,191 + Shewana3_3019 0.85 -5.4
Or see this region's nucleotide sequence