Experiment: Glycine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2568 and Shewana3_2569 are separated by 433 nucleotides Shewana3_2569 and rrmA are separated by 306 nucleotides rrmA and Shewana3_2571 are separated by 33 nucleotides
Shewana3_2568: Shewana3_2568 - sulphate transporter (RefSeq), at 3,071,714 to 3,073,273
_2568
Shewana3_2569: Shewana3_2569 - cold-shock DNA-binding protein family protein (RefSeq), at 3,073,707 to 3,073,916
_2569
Shewana3_2570: rrmA - 23S rRNA methyltransferase A (RefSeq), at 3,074,223 to 3,075,038
rrmA
Shewana3_2571: Shewana3_2571 - hypothetical protein (RefSeq), at 3,075,072 to 3,075,878
_2571
Position (kb)
3074
3075
3076 Strain fitness (log2 ratio)
-2
-1
0
1 at 3073.473 kb on + strand at 3073.497 kb on - strand at 3074.282 kb on + strand at 3074.492 kb on - strand, within rrmA at 3074.674 kb on + strand, within rrmA at 3074.674 kb on + strand, within rrmA at 3074.700 kb on - strand, within rrmA at 3074.745 kb on + strand, within rrmA at 3075.139 kb on - strand at 3075.262 kb on + strand, within Shewana3_2571 at 3075.591 kb on + strand, within Shewana3_2571 at 3075.810 kb on + strand at 3075.811 kb on + strand at 3075.811 kb on + strand at 3075.819 kb on - strand at 3075.819 kb on - strand at 3075.828 kb on + strand at 3075.841 kb on - strand at 3075.893 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Glycine (N) remove 3,073,473 + +1.3 3,073,497 - +0.1 3,074,282 + -1.7 3,074,492 - rrmA Shewana3_2570 0.33 +1.0 3,074,674 + rrmA Shewana3_2570 0.55 +0.3 3,074,674 + rrmA Shewana3_2570 0.55 +0.5 3,074,700 - rrmA Shewana3_2570 0.58 +0.0 3,074,745 + rrmA Shewana3_2570 0.64 -0.7 3,075,139 - -1.7 3,075,262 + Shewana3_2571 0.24 -0.6 3,075,591 + Shewana3_2571 0.64 +1.4 3,075,810 + -1.3 3,075,811 + -1.0 3,075,811 + +0.2 3,075,819 - -1.3 3,075,819 - -2.5 3,075,828 + +0.3 3,075,841 - +0.1 3,075,893 + -1.5
Or see this region's nucleotide sequence