Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3119 and Shew_3120 are separated by 576 nucleotides Shew_3120 and Shew_3121 are separated by 152 nucleotides Shew_3121 and Shew_3122 are separated by 168 nucleotides
Shew_3119: Shew_3119 - hypothetical protein (RefSeq), at 3,706,698 to 3,707,411
_3119
Shew_3120: Shew_3120 - phosphate-selective porin O and P (RefSeq), at 3,707,988 to 3,709,160
_3120
Shew_3121: Shew_3121 - FMN-binding domain-containing protein (RefSeq), at 3,709,313 to 3,709,828
_3121
Shew_3122: Shew_3122 - PepSY-associated TM helix domain-containing protein (RefSeq), at 3,709,997 to 3,710,797
_3122
Position (kb)
3707
3708
3709
3710 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3707.119 kb on - strand, within Shew_3119 at 3707.480 kb on + strand at 3708.015 kb on + strand at 3708.166 kb on + strand, within Shew_3120 at 3708.364 kb on - strand, within Shew_3120 at 3708.409 kb on - strand, within Shew_3120 at 3708.409 kb on - strand, within Shew_3120 at 3708.619 kb on + strand, within Shew_3120 at 3708.761 kb on + strand, within Shew_3120 at 3708.930 kb on + strand, within Shew_3120 at 3709.090 kb on - strand at 3709.240 kb on - strand at 3709.326 kb on + strand at 3709.452 kb on + strand, within Shew_3121 at 3709.644 kb on - strand, within Shew_3121 at 3709.797 kb on + strand at 3709.860 kb on - strand at 3709.896 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 3,707,119 - Shew_3119 0.59 -0.1 3,707,480 + +0.7 3,708,015 + -0.4 3,708,166 + Shew_3120 0.15 +0.1 3,708,364 - Shew_3120 0.32 -0.5 3,708,409 - Shew_3120 0.36 +1.0 3,708,409 - Shew_3120 0.36 +0.1 3,708,619 + Shew_3120 0.54 +0.2 3,708,761 + Shew_3120 0.66 -0.5 3,708,930 + Shew_3120 0.80 -1.4 3,709,090 - -1.1 3,709,240 - -0.2 3,709,326 + -1.9 3,709,452 + Shew_3121 0.27 -0.5 3,709,644 - Shew_3121 0.64 +0.7 3,709,797 + -0.2 3,709,860 - +1.3 3,709,896 + -3.1
Or see this region's nucleotide sequence