Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2432 and Shew_2433 are separated by 84 nucleotides Shew_2433 and Shew_2434 overlap by 8 nucleotides Shew_2434 and Shew_2435 are separated by 386 nucleotides
Shew_2432: Shew_2432 - hypothetical protein (RefSeq), at 2,858,632 to 2,860,272
_2432
Shew_2433: Shew_2433 - RNA polymerase sigma factor (RefSeq), at 2,860,357 to 2,860,920
_2433
Shew_2434: Shew_2434 - hypothetical protein (RefSeq), at 2,860,913 to 2,861,617
_2434
Shew_2435: Shew_2435 - sodium/proline symporter (RefSeq), at 2,862,004 to 2,863,455
_2435
Position (kb)
2860
2861
2862 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2860.113 kb on - strand at 2860.120 kb on - strand at 2861.144 kb on + strand, within Shew_2434 at 2861.321 kb on + strand, within Shew_2434 at 2861.573 kb on + strand at 2861.581 kb on - strand at 2861.639 kb on + strand at 2861.959 kb on - strand at 2861.989 kb on - strand at 2862.456 kb on - strand, within Shew_2435 at 2862.520 kb on + strand, within Shew_2435 at 2862.520 kb on + strand, within Shew_2435
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 2,860,113 - +0.6 2,860,120 - -1.2 2,861,144 + Shew_2434 0.33 -2.5 2,861,321 + Shew_2434 0.58 -3.9 2,861,573 + -0.2 2,861,581 - +0.6 2,861,639 + -0.3 2,861,959 - +0.5 2,861,989 - +1.0 2,862,456 - Shew_2435 0.31 +0.4 2,862,520 + Shew_2435 0.36 +0.2 2,862,520 + Shew_2435 0.36 -3.0
Or see this region's nucleotide sequence