Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2371 and Shew_2372 are separated by 11 nucleotides Shew_2372 and Shew_2373 are separated by 299 nucleotides Shew_2373 and Shew_2374 are separated by 237 nucleotides
Shew_2371: Shew_2371 - actP-like component of L-lactate and L-malate uptake system (from data) , at 2,772,719 to 2,774,437
_2371
Shew_2372: Shew_2372 - small component of L-lactate and L-malate uptake system (from data) , at 2,774,449 to 2,774,715
_2372
Shew_2373: Shew_2373 - DSBA oxidoreductase (RefSeq), at 2,775,015 to 2,775,647
_2373
Shew_2374: Shew_2374 - NAD-dependent epimerase/dehydratase (RefSeq), at 2,775,885 to 2,776,784
_2374
Position (kb)
2775
2776 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2774.095 kb on + strand, within Shew_2371 at 2774.163 kb on - strand, within Shew_2371 at 2774.542 kb on - strand, within Shew_2372 at 2774.731 kb on - strand at 2774.772 kb on + strand at 2774.984 kb on + strand at 2775.213 kb on - strand, within Shew_2373 at 2775.316 kb on - strand, within Shew_2373 at 2775.562 kb on + strand, within Shew_2373 at 2775.570 kb on - strand, within Shew_2373 at 2775.570 kb on - strand, within Shew_2373 at 2775.853 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 2,774,095 + Shew_2371 0.80 +1.2 2,774,163 - Shew_2371 0.84 +0.7 2,774,542 - Shew_2372 0.35 -0.4 2,774,731 - -0.3 2,774,772 + +1.8 2,774,984 + -0.7 2,775,213 - Shew_2373 0.31 +0.0 2,775,316 - Shew_2373 0.48 -0.4 2,775,562 + Shew_2373 0.86 +1.3 2,775,570 - Shew_2373 0.88 -3.0 2,775,570 - Shew_2373 0.88 +0.5 2,775,853 - -0.2
Or see this region's nucleotide sequence