Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2136 and Shew_2137 are separated by 191 nucleotides Shew_2137 and Shew_2138 are separated by 44 nucleotides Shew_2138 and Shew_2139 are separated by 97 nucleotides Shew_2139 and Shew_2140 are separated by 74 nucleotides
Shew_2136: Shew_2136 - carboxylesterase (RefSeq), at 2,483,355 to 2,484,029
_2136
Shew_2137: Shew_2137 - glutathione S-transferase domain-containing protein (RefSeq), at 2,484,221 to 2,485,240
_2137
Shew_2138: Shew_2138 - hypothetical protein (RefSeq), at 2,485,285 to 2,485,509
_2138
Shew_2139: Shew_2139 - peptidylprolyl isomerase, FKBP-type (RefSeq), at 2,485,607 to 2,486,089
_2139
Shew_2140: Shew_2140 - hypothetical protein (RefSeq), at 2,486,164 to 2,487,036
_2140
Position (kb)
2484
2485
2486 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2483.225 kb on - strand at 2483.506 kb on + strand, within Shew_2136 at 2483.514 kb on - strand, within Shew_2136 at 2483.514 kb on - strand, within Shew_2136 at 2483.708 kb on + strand, within Shew_2136 at 2483.716 kb on - strand, within Shew_2136 at 2484.057 kb on + strand at 2484.057 kb on + strand at 2484.087 kb on + strand at 2484.277 kb on - strand at 2485.087 kb on + strand, within Shew_2137 at 2485.692 kb on - strand, within Shew_2139 at 2485.781 kb on + strand, within Shew_2139 at 2485.940 kb on - strand, within Shew_2139 at 2486.008 kb on - strand, within Shew_2139
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 2,483,225 - -0.2 2,483,506 + Shew_2136 0.22 +0.7 2,483,514 - Shew_2136 0.24 +0.2 2,483,514 - Shew_2136 0.24 +0.2 2,483,708 + Shew_2136 0.52 -0.4 2,483,716 - Shew_2136 0.53 -0.1 2,484,057 + -1.5 2,484,057 + +0.1 2,484,087 + +0.1 2,484,277 - -1.5 2,485,087 + Shew_2137 0.85 +0.8 2,485,692 - Shew_2139 0.18 +0.6 2,485,781 + Shew_2139 0.36 +2.0 2,485,940 - Shew_2139 0.69 -2.1 2,486,008 - Shew_2139 0.83 +0.4
Or see this region's nucleotide sequence