Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2132 and ddl are separated by 141 nucleotides ddl and Shew_2134 are separated by 94 nucleotides Shew_2134 and ushA are separated by 400 nucleotides
Shew_2132: Shew_2132 - hypothetical protein (RefSeq), at 2,478,045 to 2,478,968
_2132
Shew_2133: ddl - D-alanyl-alanine synthetase A (RefSeq), at 2,479,110 to 2,480,114
ddl
Shew_2134: Shew_2134 - heat shock protein Hsp20 (RefSeq), at 2,480,209 to 2,480,646
_2134
Shew_2135: ushA - bifunctional UDP-sugar hydrolase/5'-nucleotidase periplasmic precursor (RefSeq), at 2,481,047 to 2,482,750
ushA
Position (kb)
2479
2480
2481 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2478.301 kb on - strand, within Shew_2132 at 2478.399 kb on + strand, within Shew_2132 at 2478.594 kb on + strand, within Shew_2132 at 2480.523 kb on + strand, within Shew_2134 at 2480.531 kb on - strand, within Shew_2134 at 2480.594 kb on - strand, within Shew_2134 at 2480.655 kb on - strand at 2480.760 kb on - strand at 2480.809 kb on - strand at 2480.907 kb on - strand at 2480.951 kb on - strand at 2480.974 kb on + strand at 2480.974 kb on + strand at 2481.076 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 2,478,301 - Shew_2132 0.28 +0.7 2,478,399 + Shew_2132 0.38 -1.4 2,478,594 + Shew_2132 0.59 +1.0 2,480,523 + Shew_2134 0.72 -0.6 2,480,531 - Shew_2134 0.74 +1.3 2,480,594 - Shew_2134 0.88 -3.4 2,480,655 - +0.0 2,480,760 - +0.1 2,480,809 - +2.0 2,480,907 - +0.0 2,480,951 - +0.1 2,480,974 + +0.2 2,480,974 + +0.1 2,481,076 - -3.1
Or see this region's nucleotide sequence