Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2121 and Shew_2122 overlap by 4 nucleotides Shew_2122 and Shew_2123 are separated by 38 nucleotides Shew_2123 and Shew_2124 are separated by 196 nucleotides
Shew_2121: Shew_2121 - diguanylate cyclase (RefSeq), at 2,457,561 to 2,458,919
_2121
Shew_2122: Shew_2122 - putative sulfite oxidase subunit YedZ (RefSeq), at 2,458,916 to 2,459,533
_2122
Shew_2123: Shew_2123 - putative sulfite oxidase subunit YedY (RefSeq), at 2,459,572 to 2,460,582
_2123
Shew_2124: Shew_2124 - hypothetical protein (RefSeq), at 2,460,779 to 2,462,254
_2124
Position (kb)
2459
2460
2461 Strain fitness (log2 ratio)
-2
-1
0
1 at 2458.595 kb on + strand, within Shew_2121 at 2458.779 kb on - strand, within Shew_2121 at 2458.996 kb on + strand, within Shew_2122 at 2459.089 kb on + strand, within Shew_2122 at 2459.166 kb on - strand, within Shew_2122 at 2459.399 kb on - strand, within Shew_2122 at 2459.874 kb on - strand, within Shew_2123 at 2460.351 kb on + strand, within Shew_2123 at 2460.755 kb on - strand at 2460.845 kb on - strand at 2460.905 kb on - strand at 2460.906 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 2,458,595 + Shew_2121 0.76 -0.7 2,458,779 - Shew_2121 0.90 +0.8 2,458,996 + Shew_2122 0.13 -0.4 2,459,089 + Shew_2122 0.28 +0.5 2,459,166 - Shew_2122 0.40 -0.5 2,459,399 - Shew_2122 0.78 +0.0 2,459,874 - Shew_2123 0.30 -2.1 2,460,351 + Shew_2123 0.77 -1.9 2,460,755 - -1.2 2,460,845 - -0.0 2,460,905 - -0.0 2,460,906 - +0.9
Or see this region's nucleotide sequence