Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aspS and Shew_2080 are separated by 169 nucleotides Shew_2080 and Shew_2081 are separated by 366 nucleotides
Shew_2079: aspS - aspartyl-tRNA synthetase (RefSeq), at 2,402,603 to 2,404,381
aspS
Shew_2080: Shew_2080 - diguanylate cyclase (RefSeq), at 2,404,551 to 2,407,355
_2080
Shew_2081: Shew_2081 - putative methyltransferase (RefSeq), at 2,407,722 to 2,408,453
_2081
Position (kb)
2404
2405
2406
2407
2408 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2404.620 kb on - strand at 2404.661 kb on + strand at 2404.661 kb on + strand at 2404.661 kb on + strand at 2404.661 kb on + strand at 2404.661 kb on + strand at 2404.669 kb on - strand at 2404.669 kb on - strand at 2404.669 kb on - strand at 2404.669 kb on - strand at 2404.763 kb on - strand at 2404.822 kb on + strand at 2404.855 kb on + strand, within Shew_2080 at 2405.016 kb on + strand, within Shew_2080 at 2405.059 kb on - strand, within Shew_2080 at 2405.059 kb on - strand, within Shew_2080 at 2405.174 kb on - strand, within Shew_2080 at 2405.236 kb on - strand, within Shew_2080 at 2405.410 kb on + strand, within Shew_2080 at 2405.607 kb on + strand, within Shew_2080 at 2405.846 kb on + strand, within Shew_2080 at 2406.027 kb on - strand, within Shew_2080 at 2406.204 kb on - strand, within Shew_2080 at 2406.338 kb on + strand, within Shew_2080 at 2406.439 kb on - strand, within Shew_2080 at 2406.597 kb on - strand, within Shew_2080 at 2406.799 kb on - strand, within Shew_2080 at 2406.998 kb on + strand, within Shew_2080 at 2406.998 kb on + strand, within Shew_2080 at 2407.006 kb on - strand, within Shew_2080 at 2407.006 kb on - strand, within Shew_2080 at 2407.117 kb on - strand at 2407.242 kb on - strand at 2407.351 kb on + strand at 2407.410 kb on - strand at 2407.422 kb on + strand at 2407.487 kb on - strand at 2407.539 kb on - strand at 2407.654 kb on + strand at 2407.662 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 2,404,620 - +0.4 2,404,661 + -3.2 2,404,661 + +0.6 2,404,661 + +2.3 2,404,661 + -0.6 2,404,661 + -3.0 2,404,669 - +0.3 2,404,669 - -0.4 2,404,669 - +0.5 2,404,669 - +0.7 2,404,763 - +0.4 2,404,822 + +0.1 2,404,855 + Shew_2080 0.11 +0.4 2,405,016 + Shew_2080 0.17 +0.4 2,405,059 - Shew_2080 0.18 +0.6 2,405,059 - Shew_2080 0.18 -1.2 2,405,174 - Shew_2080 0.22 +1.1 2,405,236 - Shew_2080 0.24 +0.8 2,405,410 + Shew_2080 0.31 -1.0 2,405,607 + Shew_2080 0.38 -0.1 2,405,846 + Shew_2080 0.46 -3.6 2,406,027 - Shew_2080 0.53 -0.6 2,406,204 - Shew_2080 0.59 +0.9 2,406,338 + Shew_2080 0.64 -0.6 2,406,439 - Shew_2080 0.67 +0.3 2,406,597 - Shew_2080 0.73 -0.4 2,406,799 - Shew_2080 0.80 -0.4 2,406,998 + Shew_2080 0.87 -0.3 2,406,998 + Shew_2080 0.87 +0.1 2,407,006 - Shew_2080 0.88 -2.6 2,407,006 - Shew_2080 0.88 +0.8 2,407,117 - -2.2 2,407,242 - +0.4 2,407,351 + +4.1 2,407,410 - -0.6 2,407,422 + +0.2 2,407,487 - +0.2 2,407,539 - +0.1 2,407,654 + -2.4 2,407,662 - -2.6
Or see this region's nucleotide sequence