Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1761 and Shew_1762 overlap by 20 nucleotides Shew_1762 and Shew_1763 are separated by 11 nucleotides Shew_1763 and Shew_1764 are separated by 10 nucleotides
Shew_1761: Shew_1761 - hypothetical protein (RefSeq), at 2,012,673 to 2,014,460
_1761
Shew_1762: Shew_1762 - hydrogenase expression/formation protein (RefSeq), at 2,014,441 to 2,015,004
_1762
Shew_1763: Shew_1763 - Ni/Fe-hydrogenase, b-type cytochrome subunit (RefSeq), at 2,015,016 to 2,015,687
_1763
Shew_1764: Shew_1764 - nickel-dependent hydrogenase, large subunit (RefSeq), at 2,015,698 to 2,017,404
_1764
Position (kb)
2014
2015
2016 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2013.843 kb on - strand, within Shew_1761 at 2013.919 kb on - strand, within Shew_1761 at 2013.965 kb on - strand, within Shew_1761 at 2013.980 kb on + strand, within Shew_1761 at 2014.472 kb on - strand at 2014.705 kb on + strand, within Shew_1762 at 2014.787 kb on - strand, within Shew_1762 at 2015.416 kb on - strand, within Shew_1763 at 2015.416 kb on - strand, within Shew_1763 at 2015.423 kb on + strand, within Shew_1763 at 2015.534 kb on + strand, within Shew_1763 at 2015.542 kb on - strand, within Shew_1763 at 2015.847 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 2,013,843 - Shew_1761 0.65 -0.9 2,013,919 - Shew_1761 0.70 +0.3 2,013,965 - Shew_1761 0.72 -0.6 2,013,980 + Shew_1761 0.73 +0.5 2,014,472 - +0.6 2,014,705 + Shew_1762 0.47 +0.3 2,014,787 - Shew_1762 0.61 +1.0 2,015,416 - Shew_1763 0.60 +0.9 2,015,416 - Shew_1763 0.60 +0.1 2,015,423 + Shew_1763 0.61 -0.1 2,015,534 + Shew_1763 0.77 +0.4 2,015,542 - Shew_1763 0.78 -2.9 2,015,847 - +1.3
Or see this region's nucleotide sequence