Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1630 and Shew_1631 are separated by 75 nucleotides Shew_1631 and Shew_1632 are separated by 95 nucleotides Shew_1632 and Shew_1633 are separated by 3 nucleotides Shew_1633 and Shew_1634 overlap by 4 nucleotides
Shew_1630: Shew_1630 - transcription-repair coupling factor (RefSeq), at 1,862,893 to 1,866,375
_1630
Shew_1631: Shew_1631 - hypothetical protein (RefSeq), at 1,866,451 to 1,867,032
_1631
Shew_1632: Shew_1632 - LolC/E family lipoprotein releasing system, transmembrane protein (RefSeq), at 1,867,128 to 1,868,360
_1632
Shew_1633: Shew_1633 - lipoprotein releasing system, ATP-binding protein (RefSeq), at 1,868,364 to 1,869,065
_1633
Shew_1634: Shew_1634 - LolC/E family lipoprotein releasing system, transmembrane protein (RefSeq), at 1,869,062 to 1,870,315
_1634
Position (kb) 1867
1868
1869 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 1866.327 kb on + strand at 1866.571 kb on + strand, within Shew_1631 at 1866.625 kb on - strand, within Shew_1631 at 1866.649 kb on + strand, within Shew_1631 at 1866.973 kb on - strand, within Shew_1631
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 1,866,327 + -4.3 1,866,571 + Shew_1631 0.21 -0.6 1,866,625 - Shew_1631 0.30 +0.7 1,866,649 + Shew_1631 0.34 +0.7 1,866,973 - Shew_1631 0.90 -0.4
Or see this region's nucleotide sequence