Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1442 and Shew_1443 are separated by 2 nucleotides Shew_1443 and Shew_1444 are separated by 83 nucleotides
Shew_1442: Shew_1442 - two component, sigma54 specific, Fis family transcriptional regulator (RefSeq), at 1,657,319 to 1,658,686
_1442
Shew_1443: Shew_1443 - integral membrane sensor signal transduction histidine kinase (RefSeq), at 1,658,689 to 1,660,512
_1443
Shew_1444: Shew_1444 - dicarboxylate TRAP transporter (succinate, fumarate, L-malate, and alpha-ketoglutarate), large permease component (from data) , at 1,660,596 to 1,661,993
_1444
Position (kb)
1658
1659
1660
1661 Strain fitness (log2 ratio)
-2
-1
0
1 at 1657.888 kb on + strand, within Shew_1442 at 1658.082 kb on + strand, within Shew_1442 at 1658.245 kb on + strand, within Shew_1442 at 1658.359 kb on - strand, within Shew_1442 at 1658.545 kb on + strand, within Shew_1442 at 1658.545 kb on + strand, within Shew_1442 at 1659.028 kb on - strand, within Shew_1443 at 1659.748 kb on + strand, within Shew_1443 at 1659.929 kb on + strand, within Shew_1443 at 1659.969 kb on + strand, within Shew_1443 at 1660.215 kb on + strand, within Shew_1443 at 1660.243 kb on + strand, within Shew_1443 at 1660.307 kb on - strand, within Shew_1443 at 1660.439 kb on - strand at 1660.834 kb on + strand, within Shew_1444
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 1,657,888 + Shew_1442 0.42 +0.1 1,658,082 + Shew_1442 0.56 +0.6 1,658,245 + Shew_1442 0.68 +1.0 1,658,359 - Shew_1442 0.76 -0.1 1,658,545 + Shew_1442 0.90 -0.4 1,658,545 + Shew_1442 0.90 +1.7 1,659,028 - Shew_1443 0.19 -0.1 1,659,748 + Shew_1443 0.58 +0.8 1,659,929 + Shew_1443 0.68 -0.5 1,659,969 + Shew_1443 0.70 -0.9 1,660,215 + Shew_1443 0.84 +0.8 1,660,243 + Shew_1443 0.85 +0.2 1,660,307 - Shew_1443 0.89 -2.0 1,660,439 - -1.6 1,660,834 + Shew_1444 0.17 +0.7
Or see this region's nucleotide sequence