Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1168 and Shew_1169 overlap by 10 nucleotides Shew_1169 and Shew_1170 overlap by 4 nucleotides
Shew_1168: Shew_1168 - glycogen branching enzyme (RefSeq), at 1,346,226 to 1,348,523
_1168
Shew_1169: Shew_1169 - glycogen debranching enzyme GlgX (RefSeq), at 1,348,514 to 1,350,715
_1169
Shew_1170: Shew_1170 - glycogen/starch/alpha-glucan phosphorylase (RefSeq), at 1,350,712 to 1,353,222
_1170
Position (kb)
1348
1349
1350
1351 Strain fitness (log2 ratio)
-2
-1
0
1 at 1347.568 kb on + strand, within Shew_1168 at 1348.081 kb on + strand, within Shew_1168 at 1348.173 kb on - strand, within Shew_1168 at 1348.422 kb on - strand at 1348.512 kb on - strand at 1348.576 kb on - strand at 1348.688 kb on + strand at 1348.740 kb on + strand, within Shew_1169 at 1348.859 kb on + strand, within Shew_1169 at 1349.340 kb on + strand, within Shew_1169 at 1349.496 kb on + strand, within Shew_1169 at 1349.693 kb on + strand, within Shew_1169 at 1349.916 kb on + strand, within Shew_1169 at 1350.149 kb on - strand, within Shew_1169 at 1350.401 kb on - strand, within Shew_1169 at 1350.430 kb on + strand, within Shew_1169 at 1350.502 kb on + strand at 1350.555 kb on + strand at 1350.647 kb on + strand at 1350.673 kb on - strand at 1350.706 kb on + strand at 1350.714 kb on - strand at 1350.714 kb on - strand at 1350.866 kb on + strand at 1350.902 kb on - strand at 1350.947 kb on - strand at 1351.016 kb on + strand, within Shew_1170 at 1351.179 kb on - strand, within Shew_1170 at 1351.311 kb on + strand, within Shew_1170 at 1351.409 kb on + strand, within Shew_1170 at 1351.524 kb on + strand, within Shew_1170 at 1351.590 kb on + strand, within Shew_1170 at 1351.682 kb on + strand, within Shew_1170
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 1,347,568 + Shew_1168 0.58 -0.4 1,348,081 + Shew_1168 0.81 -1.3 1,348,173 - Shew_1168 0.85 -0.3 1,348,422 - -0.7 1,348,512 - -0.9 1,348,576 - +0.0 1,348,688 + +0.1 1,348,740 + Shew_1169 0.10 +0.3 1,348,859 + Shew_1169 0.16 -0.4 1,349,340 + Shew_1169 0.38 +0.4 1,349,496 + Shew_1169 0.45 -0.1 1,349,693 + Shew_1169 0.54 -1.2 1,349,916 + Shew_1169 0.64 +0.2 1,350,149 - Shew_1169 0.74 -0.5 1,350,401 - Shew_1169 0.86 -2.5 1,350,430 + Shew_1169 0.87 +1.3 1,350,502 + +0.1 1,350,555 + -0.5 1,350,647 + -1.0 1,350,673 - +1.0 1,350,706 + -0.3 1,350,714 - +0.7 1,350,714 - +0.5 1,350,866 + -1.0 1,350,902 - -0.5 1,350,947 - -0.1 1,351,016 + Shew_1170 0.12 -0.0 1,351,179 - Shew_1170 0.19 -0.1 1,351,311 + Shew_1170 0.24 -0.6 1,351,409 + Shew_1170 0.28 +0.2 1,351,524 + Shew_1170 0.32 -2.5 1,351,590 + Shew_1170 0.35 +1.0 1,351,682 + Shew_1170 0.39 +0.8
Or see this region's nucleotide sequence