Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1076 and Shew_1077 are separated by 75 nucleotides Shew_1077 and Shew_1078 are separated by 130 nucleotides Shew_1078 and thrA are separated by 717 nucleotides
Shew_1076: Shew_1076 - Trp operon repressor (RefSeq), at 1,239,375 to 1,239,728
_1076
Shew_1077: Shew_1077 - hypothetical protein (RefSeq), at 1,239,804 to 1,240,235
_1077
Shew_1078: Shew_1078 - peptidylprolyl isomerase, FKBP-type (RefSeq), at 1,240,366 to 1,240,980
_1078
Shew_1079: thrA - bifunctional aspartokinase I/homeserine dehydrogenase I (RefSeq), at 1,241,698 to 1,244,166
thrA
Position (kb)
1240
1241 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1239.549 kb on - strand, within Shew_1076 at 1239.580 kb on - strand, within Shew_1076 at 1239.870 kb on - strand, within Shew_1077 at 1239.882 kb on + strand, within Shew_1077 at 1240.232 kb on - strand at 1240.783 kb on - strand, within Shew_1078 at 1240.842 kb on + strand, within Shew_1078 at 1240.971 kb on - strand at 1241.040 kb on - strand at 1241.041 kb on - strand at 1241.086 kb on + strand at 1241.094 kb on - strand at 1241.099 kb on - strand at 1241.189 kb on + strand at 1241.197 kb on - strand at 1241.461 kb on + strand at 1241.496 kb on + strand at 1241.682 kb on + strand at 1241.690 kb on - strand at 1241.690 kb on - strand at 1241.753 kb on - strand at 1241.907 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 1,239,549 - Shew_1076 0.49 -3.2 1,239,580 - Shew_1076 0.58 -0.3 1,239,870 - Shew_1077 0.15 -0.3 1,239,882 + Shew_1077 0.18 -1.3 1,240,232 - +0.3 1,240,783 - Shew_1078 0.68 +2.5 1,240,842 + Shew_1078 0.77 +2.6 1,240,971 - +2.7 1,241,040 - +1.5 1,241,041 - +2.8 1,241,086 + -1.4 1,241,094 - +0.1 1,241,099 - +0.2 1,241,189 + -2.0 1,241,197 - +0.6 1,241,461 + +0.3 1,241,496 + -0.8 1,241,682 + +0.5 1,241,690 - +0.6 1,241,690 - -0.4 1,241,753 - -0.0 1,241,907 + -0.2
Or see this region's nucleotide sequence