Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nrfA and Shew_0845 are separated by 74 nucleotides Shew_0845 and Shew_0846 are separated by 252 nucleotides Shew_0846 and Shew_0847 are separated by 12 nucleotides
Shew_0844: nrfA - cytochrome c nitrite reductase (RefSeq), at 967,114 to 968,514
nrfA
Shew_0845: Shew_0845 - isochorismatase hydrolase (RefSeq), at 968,589 to 969,131
_0845
Shew_0846: Shew_0846 - nitrogen regulatory protein P-II (RefSeq), at 969,384 to 969,722
_0846
Shew_0847: Shew_0847 - Rh family protein/ammonium transporter (RefSeq), at 969,735 to 970,985
_0847
Position (kb) 968
969
970 Strain fitness (log2 ratio) -1
0
1 at 967.595 kb on + strand, within nrfA at 967.686 kb on + strand, within nrfA at 967.721 kb on + strand, within nrfA at 967.791 kb on + strand, within nrfA at 967.911 kb on + strand, within nrfA at 968.054 kb on + strand, within nrfA at 968.141 kb on - strand, within nrfA at 968.361 kb on + strand, within nrfA at 968.361 kb on + strand, within nrfA at 968.524 kb on + strand at 968.634 kb on - strand at 968.762 kb on + strand, within Shew_0845 at 968.981 kb on + strand, within Shew_0845 at 969.098 kb on - strand at 969.379 kb on - strand at 969.447 kb on + strand, within Shew_0846 at 969.505 kb on + strand, within Shew_0846 at 969.564 kb on - strand, within Shew_0846 at 970.128 kb on - strand, within Shew_0847
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 967,595 + nrfA Shew_0844 0.34 -0.6 967,686 + nrfA Shew_0844 0.41 +0.2 967,721 + nrfA Shew_0844 0.43 -0.3 967,791 + nrfA Shew_0844 0.48 +0.5 967,911 + nrfA Shew_0844 0.57 -0.3 968,054 + nrfA Shew_0844 0.67 +1.3 968,141 - nrfA Shew_0844 0.73 -1.5 968,361 + nrfA Shew_0844 0.89 +0.8 968,361 + nrfA Shew_0844 0.89 -0.9 968,524 + -0.3 968,634 - -1.4 968,762 + Shew_0845 0.32 -0.5 968,981 + Shew_0845 0.72 -0.1 969,098 - -0.3 969,379 - -0.8 969,447 + Shew_0846 0.19 -1.4 969,505 + Shew_0846 0.36 -1.4 969,564 - Shew_0846 0.53 +0.6 970,128 - Shew_0847 0.31 -0.2
Or see this region's nucleotide sequence