Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0113 and Shew_0114 are separated by 21 nucleotides Shew_0114 and Shew_0115 overlap by 4 nucleotides Shew_0115 and Shew_0116 are separated by 163 nucleotides Shew_0116 and Shew_0117 are separated by 4 nucleotides Shew_0117 and Shew_0118 are separated by 12 nucleotides
Shew_0113: Shew_0113 - CheA signal transduction histidine kinase (RefSeq), at 124,836 to 126,896
_0113
Shew_0114: Shew_0114 - response regulator receiver protein (RefSeq), at 126,918 to 127,280
_0114
Shew_0115: Shew_0115 - hypothetical protein (RefSeq), at 127,277 to 127,576
_0115
Shew_0116: Shew_0116 - electron transport protein SCO1/SenC (RefSeq), at 127,740 to 128,375
_0116
Shew_0117: Shew_0117 - protoheme IX farnesyltransferase (RefSeq), at 128,380 to 129,282
_0117
Shew_0118: Shew_0118 - cytochrome oxidase assembly (RefSeq), at 129,295 to 130,278
_0118
Position (kb) 127
128
129 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 126.842 kb on + strand at 126.921 kb on + strand at 126.921 kb on + strand at 126.974 kb on - strand, within Shew_0114 at 127.124 kb on + strand, within Shew_0114 at 127.207 kb on - strand, within Shew_0114 at 127.228 kb on - strand, within Shew_0114 at 127.350 kb on - strand, within Shew_0115 at 127.817 kb on + strand, within Shew_0116 at 127.874 kb on - strand, within Shew_0116 at 127.933 kb on + strand, within Shew_0116 at 128.238 kb on + strand, within Shew_0116 at 128.260 kb on - strand, within Shew_0116 at 128.324 kb on + strand at 128.349 kb on + strand at 128.526 kb on - strand, within Shew_0117 at 128.534 kb on - strand, within Shew_0117 at 128.568 kb on + strand, within Shew_0117 at 128.568 kb on + strand, within Shew_0117 at 128.571 kb on - strand, within Shew_0117 at 128.573 kb on + strand, within Shew_0117 at 128.573 kb on + strand, within Shew_0117 at 128.573 kb on + strand, within Shew_0117 at 128.576 kb on - strand, within Shew_0117 at 128.576 kb on - strand, within Shew_0117 at 128.576 kb on - strand, within Shew_0117 at 128.581 kb on - strand, within Shew_0117 at 128.581 kb on - strand, within Shew_0117 at 128.581 kb on - strand, within Shew_0117 at 128.581 kb on - strand, within Shew_0117 at 128.603 kb on + strand, within Shew_0117 at 128.904 kb on - strand, within Shew_0117 at 128.930 kb on + strand, within Shew_0117 at 128.943 kb on + strand, within Shew_0117 at 129.133 kb on + strand, within Shew_0117 at 129.209 kb on - strand at 129.254 kb on + strand at 129.312 kb on + strand at 129.335 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 126,842 + -0.5 126,921 + +0.3 126,921 + -3.8 126,974 - Shew_0114 0.15 +1.0 127,124 + Shew_0114 0.57 -2.5 127,207 - Shew_0114 0.80 +0.1 127,228 - Shew_0114 0.85 +0.5 127,350 - Shew_0115 0.24 -1.6 127,817 + Shew_0116 0.12 +0.2 127,874 - Shew_0116 0.21 -1.3 127,933 + Shew_0116 0.30 +0.5 128,238 + Shew_0116 0.78 -0.9 128,260 - Shew_0116 0.82 -2.9 128,324 + +0.3 128,349 + -0.9 128,526 - Shew_0117 0.16 +0.4 128,534 - Shew_0117 0.17 -1.7 128,568 + Shew_0117 0.21 -0.3 128,568 + Shew_0117 0.21 +0.4 128,571 - Shew_0117 0.21 -0.6 128,573 + Shew_0117 0.21 +0.5 128,573 + Shew_0117 0.21 +0.2 128,573 + Shew_0117 0.21 -0.4 128,576 - Shew_0117 0.22 -3.8 128,576 - Shew_0117 0.22 +0.5 128,576 - Shew_0117 0.22 -0.7 128,581 - Shew_0117 0.22 +0.9 128,581 - Shew_0117 0.22 -0.7 128,581 - Shew_0117 0.22 +1.0 128,581 - Shew_0117 0.22 +0.2 128,603 + Shew_0117 0.25 -0.0 128,904 - Shew_0117 0.58 +1.0 128,930 + Shew_0117 0.61 +0.9 128,943 + Shew_0117 0.62 +0.5 129,133 + Shew_0117 0.83 +0.2 129,209 - +0.2 129,254 + -0.3 129,312 + +0.8 129,335 - -0.1
Or see this region's nucleotide sequence