Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0063 and Shew_0064 are separated by 20 nucleotides Shew_0064 and Shew_0065 are separated by 68 nucleotides Shew_0065 and Shew_0066 are separated by 274 nucleotides Shew_0066 and Shew_0067 are separated by 1 nucleotides
Shew_0063: Shew_0063 - molybdopterin oxidoreductase (RefSeq), at 75,104 to 77,959
_0063
Shew_0064: Shew_0064 - 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein (RefSeq), at 77,980 to 78,549
_0064
Shew_0065: Shew_0065 - formate dehydrogenase, gamma subunit (RefSeq), at 78,618 to 79,610
_0065
Shew_0066: Shew_0066 - hypothetical protein (RefSeq), at 79,885 to 80,250
_0066
Shew_0067: Shew_0067 - hypothetical protein (RefSeq), at 80,252 to 80,761
_0067
Position (kb) 78
79
80 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 77.621 kb on - strand, within Shew_0063 at 77.751 kb on - strand at 77.794 kb on - strand at 77.882 kb on + strand at 77.928 kb on + strand at 78.067 kb on + strand, within Shew_0064 at 78.075 kb on - strand, within Shew_0064 at 78.138 kb on - strand, within Shew_0064 at 78.265 kb on + strand, within Shew_0064 at 78.265 kb on + strand, within Shew_0064 at 78.372 kb on - strand, within Shew_0064 at 78.519 kb on - strand at 78.568 kb on - strand at 78.579 kb on - strand at 78.712 kb on + strand at 78.817 kb on + strand, within Shew_0065 at 78.853 kb on + strand, within Shew_0065 at 79.008 kb on - strand, within Shew_0065 at 79.008 kb on - strand, within Shew_0065 at 79.038 kb on + strand, within Shew_0065 at 79.106 kb on + strand, within Shew_0065 at 79.114 kb on + strand, within Shew_0065 at 79.259 kb on + strand, within Shew_0065 at 79.267 kb on - strand, within Shew_0065 at 79.414 kb on + strand, within Shew_0065 at 79.538 kb on - strand at 79.717 kb on - strand at 79.744 kb on + strand at 79.758 kb on - strand at 80.040 kb on + strand, within Shew_0066 at 80.040 kb on + strand, within Shew_0066 at 80.048 kb on - strand, within Shew_0066 at 80.110 kb on + strand, within Shew_0066 at 80.118 kb on - strand, within Shew_0066 at 80.118 kb on - strand, within Shew_0066 at 80.209 kb on - strand, within Shew_0066
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 77,621 - Shew_0063 0.88 -0.6 77,751 - -2.4 77,794 - -0.6 77,882 + -0.9 77,928 + -0.5 78,067 + Shew_0064 0.15 +0.2 78,075 - Shew_0064 0.17 -2.2 78,138 - Shew_0064 0.28 -1.4 78,265 + Shew_0064 0.50 -1.0 78,265 + Shew_0064 0.50 -0.3 78,372 - Shew_0064 0.69 -1.8 78,519 - -0.9 78,568 - +0.6 78,579 - +0.2 78,712 + -0.1 78,817 + Shew_0065 0.20 -0.5 78,853 + Shew_0065 0.24 +0.4 79,008 - Shew_0065 0.39 +1.4 79,008 - Shew_0065 0.39 -0.4 79,038 + Shew_0065 0.42 +1.2 79,106 + Shew_0065 0.49 -0.3 79,114 + Shew_0065 0.50 -1.5 79,259 + Shew_0065 0.65 +0.2 79,267 - Shew_0065 0.65 +0.1 79,414 + Shew_0065 0.80 -1.0 79,538 - -3.9 79,717 - -0.1 79,744 + -0.1 79,758 - +0.5 80,040 + Shew_0066 0.42 +1.5 80,040 + Shew_0066 0.42 +0.3 80,048 - Shew_0066 0.45 +0.2 80,110 + Shew_0066 0.61 -1.3 80,118 - Shew_0066 0.64 -2.5 80,118 - Shew_0066 0.64 +0.4 80,209 - Shew_0066 0.89 -0.0
Or see this region's nucleotide sequence