Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0032 and Shew_0033 are separated by 222 nucleotides Shew_0033 and Shew_0034 are separated by 61 nucleotides
Shew_0032: Shew_0032 - hydrophobe/amphiphile efflux-1 (HAE1) family protein (RefSeq), at 34,426 to 37,575
_0032
Shew_0033: Shew_0033 - peptidase M14, carboxypeptidase A (RefSeq), at 37,798 to 39,639
_0033
Shew_0034: Shew_0034 - methyltransferase type 12 (RefSeq), at 39,701 to 40,504
_0034
Position (kb) 37
38
39
40 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 37.182 kb on + strand, within Shew_0032 at 37.182 kb on + strand, within Shew_0032 at 37.207 kb on + strand, within Shew_0032 at 37.296 kb on + strand at 37.960 kb on + strand at 37.968 kb on - strand at 38.118 kb on - strand, within Shew_0033 at 38.295 kb on + strand, within Shew_0033 at 38.564 kb on + strand, within Shew_0033 at 38.869 kb on + strand, within Shew_0033 at 38.881 kb on + strand, within Shew_0033 at 38.998 kb on + strand, within Shew_0033 at 39.076 kb on + strand, within Shew_0033 at 39.084 kb on - strand, within Shew_0033 at 39.100 kb on - strand, within Shew_0033 at 39.145 kb on - strand, within Shew_0033 at 39.195 kb on + strand, within Shew_0033 at 39.195 kb on + strand, within Shew_0033 at 39.195 kb on + strand, within Shew_0033 at 39.203 kb on - strand, within Shew_0033 at 39.211 kb on - strand, within Shew_0033 at 39.211 kb on - strand, within Shew_0033 at 39.609 kb on + strand at 39.628 kb on - strand at 40.137 kb on + strand, within Shew_0034 at 40.145 kb on - strand, within Shew_0034 at 40.320 kb on + strand, within Shew_0034
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 37,182 + Shew_0032 0.87 -3.9 37,182 + Shew_0032 0.87 -1.2 37,207 + Shew_0032 0.88 -4.2 37,296 + -1.3 37,960 + -0.0 37,968 - -1.3 38,118 - Shew_0033 0.17 +0.1 38,295 + Shew_0033 0.27 +0.9 38,564 + Shew_0033 0.42 +0.1 38,869 + Shew_0033 0.58 -0.2 38,881 + Shew_0033 0.59 +0.5 38,998 + Shew_0033 0.65 -0.2 39,076 + Shew_0033 0.69 -1.4 39,084 - Shew_0033 0.70 +0.9 39,100 - Shew_0033 0.71 -1.6 39,145 - Shew_0033 0.73 +0.0 39,195 + Shew_0033 0.76 +0.3 39,195 + Shew_0033 0.76 -0.0 39,195 + Shew_0033 0.76 -2.4 39,203 - Shew_0033 0.76 -1.1 39,211 - Shew_0033 0.77 +0.1 39,211 - Shew_0033 0.77 +0.1 39,609 + +0.3 39,628 - -0.2 40,137 + Shew_0034 0.54 +0.4 40,145 - Shew_0034 0.55 -0.0 40,320 + Shew_0034 0.77 -0.4
Or see this region's nucleotide sequence