Experiment: D-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_887 and Pf6N2E2_888 are separated by 365 nucleotides Pf6N2E2_888 and Pf6N2E2_889 are separated by 410 nucleotides
Pf6N2E2_887 - hypothetical protein, at 1,040,365 to 1,042,548
_887
Pf6N2E2_888 - Enoyl-CoA hydratase (EC 4.2.1.17), at 1,042,914 to 1,043,459
_888
Pf6N2E2_889 - Hydrolases of the alpha/beta superfamily, at 1,043,870 to 1,044,631
_889
Position (kb)
1042
1043
1044 Strain fitness (log2 ratio)
-1
0
1 at 1041.938 kb on - strand, within Pf6N2E2_887 at 1042.112 kb on + strand, within Pf6N2E2_887 at 1042.112 kb on + strand, within Pf6N2E2_887 at 1042.113 kb on - strand, within Pf6N2E2_887 at 1042.113 kb on - strand, within Pf6N2E2_887 at 1042.160 kb on - strand, within Pf6N2E2_887 at 1042.371 kb on - strand at 1042.416 kb on + strand at 1042.416 kb on + strand at 1042.416 kb on + strand at 1042.417 kb on - strand at 1042.417 kb on - strand at 1042.417 kb on - strand at 1042.417 kb on - strand at 1042.447 kb on + strand at 1042.447 kb on + strand at 1042.447 kb on + strand at 1042.448 kb on - strand at 1042.448 kb on - strand at 1042.448 kb on - strand at 1042.450 kb on + strand at 1042.538 kb on + strand at 1042.539 kb on - strand at 1042.546 kb on + strand at 1042.547 kb on - strand at 1042.673 kb on + strand at 1042.733 kb on + strand at 1042.733 kb on + strand at 1042.734 kb on - strand at 1042.734 kb on - strand at 1043.279 kb on - strand, within Pf6N2E2_888 at 1043.301 kb on + strand, within Pf6N2E2_888 at 1043.302 kb on - strand, within Pf6N2E2_888 at 1043.355 kb on + strand, within Pf6N2E2_888 at 1043.355 kb on + strand, within Pf6N2E2_888 at 1043.355 kb on + strand, within Pf6N2E2_888 at 1043.407 kb on - strand at 1043.462 kb on - strand at 1043.462 kb on - strand at 1043.668 kb on + strand at 1043.668 kb on + strand at 1043.668 kb on + strand at 1043.669 kb on - strand at 1043.685 kb on + strand at 1043.685 kb on + strand at 1043.686 kb on - strand at 1043.686 kb on - strand at 1043.798 kb on + strand at 1043.798 kb on + strand at 1043.798 kb on + strand at 1043.799 kb on - strand at 1043.961 kb on + strand, within Pf6N2E2_889 at 1043.961 kb on + strand, within Pf6N2E2_889 at 1043.961 kb on + strand, within Pf6N2E2_889 at 1043.961 kb on + strand, within Pf6N2E2_889 at 1043.961 kb on + strand, within Pf6N2E2_889 at 1043.961 kb on + strand, within Pf6N2E2_889 at 1043.961 kb on + strand, within Pf6N2E2_889 at 1043.962 kb on - strand, within Pf6N2E2_889 at 1043.962 kb on - strand, within Pf6N2E2_889 at 1044.288 kb on + strand, within Pf6N2E2_889 at 1044.302 kb on + strand, within Pf6N2E2_889 at 1044.427 kb on + strand, within Pf6N2E2_889 at 1044.427 kb on + strand, within Pf6N2E2_889 at 1044.428 kb on - strand, within Pf6N2E2_889
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (C) remove 1,041,938 - Pf6N2E2_887 0.72 -0.0 1,042,112 + Pf6N2E2_887 0.80 +1.1 1,042,112 + Pf6N2E2_887 0.80 -0.2 1,042,113 - Pf6N2E2_887 0.80 -0.8 1,042,113 - Pf6N2E2_887 0.80 +0.3 1,042,160 - Pf6N2E2_887 0.82 +1.0 1,042,371 - +0.1 1,042,416 + -0.4 1,042,416 + +0.0 1,042,416 + +1.2 1,042,417 - +0.5 1,042,417 - +1.4 1,042,417 - -1.4 1,042,417 - -0.0 1,042,447 + -0.1 1,042,447 + -0.2 1,042,447 + +0.5 1,042,448 - +0.7 1,042,448 - +1.0 1,042,448 - +0.4 1,042,450 + +0.3 1,042,538 + +0.5 1,042,539 - -1.5 1,042,546 + -1.0 1,042,547 - +1.3 1,042,673 + +0.2 1,042,733 + +0.6 1,042,733 + +0.1 1,042,734 - -1.0 1,042,734 - -0.3 1,043,279 - Pf6N2E2_888 0.67 +0.7 1,043,301 + Pf6N2E2_888 0.71 -0.5 1,043,302 - Pf6N2E2_888 0.71 -1.0 1,043,355 + Pf6N2E2_888 0.81 -1.0 1,043,355 + Pf6N2E2_888 0.81 -0.9 1,043,355 + Pf6N2E2_888 0.81 -1.7 1,043,407 - +1.1 1,043,462 - -0.3 1,043,462 - +1.3 1,043,668 + +0.6 1,043,668 + +1.1 1,043,668 + -0.3 1,043,669 - +0.2 1,043,685 + -0.3 1,043,685 + +0.9 1,043,686 - +0.5 1,043,686 - -0.7 1,043,798 + +0.2 1,043,798 + -0.6 1,043,798 + -0.7 1,043,799 - +1.1 1,043,961 + Pf6N2E2_889 0.12 +0.6 1,043,961 + Pf6N2E2_889 0.12 +0.6 1,043,961 + Pf6N2E2_889 0.12 -0.2 1,043,961 + Pf6N2E2_889 0.12 -0.2 1,043,961 + Pf6N2E2_889 0.12 -1.3 1,043,961 + Pf6N2E2_889 0.12 +0.8 1,043,961 + Pf6N2E2_889 0.12 +0.2 1,043,962 - Pf6N2E2_889 0.12 +1.2 1,043,962 - Pf6N2E2_889 0.12 +0.6 1,044,288 + Pf6N2E2_889 0.55 -0.8 1,044,302 + Pf6N2E2_889 0.57 +0.3 1,044,427 + Pf6N2E2_889 0.73 +0.6 1,044,427 + Pf6N2E2_889 0.73 -0.0 1,044,428 - Pf6N2E2_889 0.73 -0.3
Or see this region's nucleotide sequence