Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_5852

Experiment: D-Alanine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_5850 and Pf6N2E2_5851 are separated by 482 nucleotidesPf6N2E2_5851 and Pf6N2E2_5852 overlap by 7 nucleotidesPf6N2E2_5852 and Pf6N2E2_5853 are separated by 3 nucleotides Pf6N2E2_5850 - Citrate synthase (si) (EC 2.3.3.1), at 6,666,617 to 6,667,906 _5850 Pf6N2E2_5851 - Succinate dehydrogenase cytochrome b-556 subunit, at 6,668,389 to 6,668,646 _5851 Pf6N2E2_5852 - Succinate dehydrogenase hydrophobic membrane anchor protein, at 6,668,640 to 6,669,008 _5852 Pf6N2E2_5853 - Succinate dehydrogenase flavoprotein subunit (EC 1.3.99.1), at 6,669,012 to 6,670,784 _5853 Position (kb) 6668 6669 6670Strain fitness (log2 ratio) -2 -1 0 1at 6667.917 kb on - strandat 6667.920 kb on - strandat 6667.920 kb on - strandat 6667.920 kb on - strandat 6667.968 kb on - strandat 6668.086 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Alanine (C)
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6,667,917 - -0.5
6,667,920 - -1.5
6,667,920 - +1.2
6,667,920 - -0.4
6,667,968 - -2.3
6,668,086 + -0.7

Or see this region's nucleotide sequence