Experiment: D-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_563 and Pf6N2E2_564 are separated by 312 nucleotides Pf6N2E2_564 and Pf6N2E2_565 are separated by 27 nucleotides Pf6N2E2_565 and Pf6N2E2_566 are separated by 165 nucleotides
Pf6N2E2_563 - Serine transporter, at 636,957 to 638,237
_563
Pf6N2E2_564 - Aconitate hydratase 2 (EC 4.2.1.3) @ 2-methylisocitrate dehydratase (EC 4.2.1.99), at 638,550 to 641,159
_564
Pf6N2E2_565 - hypothetical protein, at 641,187 to 641,402
_565
Pf6N2E2_566 - hypothetical protein, at 641,568 to 642,038
_566
Position (kb) 638
639
640
641
642 Strain fitness (log2 ratio) -2
-1
0
1
2 at 637.586 kb on + strand, within Pf6N2E2_563 at 637.586 kb on + strand, within Pf6N2E2_563 at 637.587 kb on - strand, within Pf6N2E2_563 at 637.587 kb on - strand, within Pf6N2E2_563 at 637.830 kb on + strand, within Pf6N2E2_563 at 637.921 kb on - strand, within Pf6N2E2_563 at 637.921 kb on - strand, within Pf6N2E2_563 at 637.921 kb on - strand, within Pf6N2E2_563 at 638.121 kb on + strand at 638.122 kb on - strand at 638.122 kb on - strand at 638.221 kb on - strand at 638.345 kb on + strand at 638.346 kb on - strand at 638.445 kb on + strand at 638.494 kb on + strand at 638.494 kb on + strand at 638.495 kb on - strand at 638.495 kb on - strand at 638.545 kb on - strand at 638.545 kb on - strand at 641.422 kb on - strand at 641.422 kb on - strand at 641.440 kb on - strand at 641.440 kb on - strand at 641.480 kb on - strand at 641.997 kb on + strand at 641.997 kb on + strand at 641.997 kb on + strand at 641.997 kb on + strand at 641.998 kb on - strand at 642.001 kb on + strand at 642.002 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (C) remove 637,586 + Pf6N2E2_563 0.49 +0.2 637,586 + Pf6N2E2_563 0.49 -0.1 637,587 - Pf6N2E2_563 0.49 +0.6 637,587 - Pf6N2E2_563 0.49 +1.3 637,830 + Pf6N2E2_563 0.68 -0.4 637,921 - Pf6N2E2_563 0.75 +0.0 637,921 - Pf6N2E2_563 0.75 +1.3 637,921 - Pf6N2E2_563 0.75 +2.2 638,121 + +0.2 638,122 - +1.3 638,122 - -1.2 638,221 - +0.5 638,345 + -1.4 638,346 - -0.3 638,445 + +0.5 638,494 + +0.1 638,494 + +0.6 638,495 - +0.4 638,495 - -0.3 638,545 - +1.2 638,545 - +2.1 641,422 - +2.5 641,422 - +0.4 641,440 - +1.6 641,440 - +1.1 641,480 - -1.4 641,997 + -0.0 641,997 + -0.1 641,997 + +0.9 641,997 + -0.3 641,998 - -0.0 642,001 + -1.8 642,002 - -1.0
Or see this region's nucleotide sequence