Experiment: D-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5488 and Pf6N2E2_5489 are separated by 244 nucleotides Pf6N2E2_5489 and Pf6N2E2_5490 are separated by 39 nucleotides Pf6N2E2_5490 and Pf6N2E2_5491 are separated by 236 nucleotides
Pf6N2E2_5488 - C4-dicarboxylate transport sensor protein, at 6,305,727 to 6,307,493
_5488
Pf6N2E2_5489 - Fumarate hydratase class I, aerobic (EC 4.2.1.2), at 6,307,738 to 6,309,261
_5489
Pf6N2E2_5490 - hypothetical protein, at 6,309,301 to 6,309,501
_5490
Pf6N2E2_5491 - FOG: GGDEF domain, at 6,309,738 to 6,310,844
_5491
Position (kb)
6307
6308
6309
6310 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 6306.781 kb on + strand, within Pf6N2E2_5488 at 6306.782 kb on - strand, within Pf6N2E2_5488 at 6307.009 kb on + strand, within Pf6N2E2_5488 at 6307.009 kb on + strand, within Pf6N2E2_5488 at 6307.010 kb on - strand, within Pf6N2E2_5488 at 6307.010 kb on - strand, within Pf6N2E2_5488 at 6307.081 kb on + strand, within Pf6N2E2_5488 at 6307.082 kb on - strand, within Pf6N2E2_5488 at 6307.096 kb on + strand, within Pf6N2E2_5488 at 6307.096 kb on + strand, within Pf6N2E2_5488 at 6307.096 kb on + strand, within Pf6N2E2_5488 at 6307.096 kb on + strand, within Pf6N2E2_5488 at 6307.096 kb on + strand, within Pf6N2E2_5488 at 6307.097 kb on - strand, within Pf6N2E2_5488 at 6307.097 kb on - strand, within Pf6N2E2_5488 at 6307.097 kb on - strand, within Pf6N2E2_5488 at 6307.097 kb on - strand, within Pf6N2E2_5488 at 6307.111 kb on + strand, within Pf6N2E2_5488 at 6307.111 kb on + strand, within Pf6N2E2_5488 at 6307.111 kb on + strand, within Pf6N2E2_5488 at 6307.111 kb on + strand, within Pf6N2E2_5488 at 6307.111 kb on + strand, within Pf6N2E2_5488 at 6307.111 kb on + strand, within Pf6N2E2_5488 at 6307.111 kb on + strand, within Pf6N2E2_5488 at 6307.111 kb on + strand, within Pf6N2E2_5488 at 6307.111 kb on + strand, within Pf6N2E2_5488 at 6307.111 kb on + strand, within Pf6N2E2_5488 at 6307.111 kb on + strand, within Pf6N2E2_5488 at 6307.111 kb on + strand, within Pf6N2E2_5488 at 6307.111 kb on + strand, within Pf6N2E2_5488 at 6307.112 kb on - strand, within Pf6N2E2_5488 at 6307.112 kb on - strand, within Pf6N2E2_5488 at 6307.112 kb on - strand, within Pf6N2E2_5488 at 6307.112 kb on - strand, within Pf6N2E2_5488 at 6307.112 kb on - strand, within Pf6N2E2_5488 at 6307.112 kb on - strand, within Pf6N2E2_5488 at 6307.112 kb on - strand, within Pf6N2E2_5488 at 6307.112 kb on - strand, within Pf6N2E2_5488 at 6307.224 kb on + strand, within Pf6N2E2_5488 at 6307.224 kb on + strand, within Pf6N2E2_5488 at 6307.224 kb on + strand, within Pf6N2E2_5488 at 6307.224 kb on + strand, within Pf6N2E2_5488 at 6307.224 kb on + strand, within Pf6N2E2_5488 at 6307.225 kb on - strand, within Pf6N2E2_5488 at 6307.225 kb on - strand, within Pf6N2E2_5488 at 6307.225 kb on - strand, within Pf6N2E2_5488 at 6307.225 kb on - strand, within Pf6N2E2_5488 at 6307.225 kb on - strand, within Pf6N2E2_5488 at 6307.225 kb on - strand, within Pf6N2E2_5488 at 6307.269 kb on + strand, within Pf6N2E2_5488 at 6307.269 kb on + strand, within Pf6N2E2_5488 at 6307.270 kb on - strand, within Pf6N2E2_5488 at 6307.270 kb on - strand, within Pf6N2E2_5488 at 6307.270 kb on - strand, within Pf6N2E2_5488 at 6307.270 kb on - strand, within Pf6N2E2_5488 at 6307.342 kb on + strand at 6307.342 kb on + strand at 6307.456 kb on + strand at 6307.456 kb on + strand at 6307.457 kb on - strand at 6307.475 kb on + strand at 6307.481 kb on + strand at 6307.481 kb on + strand at 6307.481 kb on + strand at 6307.482 kb on - strand at 6307.482 kb on - strand at 6307.563 kb on + strand at 6307.563 kb on + strand at 6307.563 kb on + strand at 6307.564 kb on - strand at 6307.739 kb on + strand at 6307.739 kb on + strand at 6307.740 kb on - strand at 6309.298 kb on - strand at 6309.298 kb on - strand at 6309.342 kb on + strand, within Pf6N2E2_5490 at 6309.343 kb on - strand, within Pf6N2E2_5490 at 6309.343 kb on - strand, within Pf6N2E2_5490 at 6309.343 kb on - strand, within Pf6N2E2_5490 at 6309.343 kb on - strand, within Pf6N2E2_5490 at 6309.343 kb on - strand, within Pf6N2E2_5490 at 6309.343 kb on - strand, within Pf6N2E2_5490 at 6309.343 kb on - strand, within Pf6N2E2_5490 at 6309.345 kb on - strand, within Pf6N2E2_5490 at 6309.345 kb on - strand, within Pf6N2E2_5490 at 6309.391 kb on - strand, within Pf6N2E2_5490 at 6309.391 kb on - strand, within Pf6N2E2_5490 at 6309.391 kb on - strand, within Pf6N2E2_5490 at 6309.391 kb on - strand, within Pf6N2E2_5490 at 6309.391 kb on - strand, within Pf6N2E2_5490 at 6309.391 kb on - strand, within Pf6N2E2_5490 at 6309.391 kb on - strand, within Pf6N2E2_5490 at 6309.391 kb on - strand, within Pf6N2E2_5490 at 6309.391 kb on - strand, within Pf6N2E2_5490 at 6309.661 kb on - strand at 6309.671 kb on - strand at 6309.671 kb on - strand at 6309.695 kb on - strand at 6309.695 kb on - strand at 6309.695 kb on - strand at 6309.695 kb on - strand at 6309.747 kb on - strand at 6309.798 kb on + strand at 6309.798 kb on + strand at 6309.799 kb on - strand at 6309.799 kb on - strand at 6309.817 kb on + strand at 6309.817 kb on + strand at 6309.846 kb on + strand at 6309.846 kb on + strand at 6309.846 kb on + strand at 6309.846 kb on + strand at 6309.846 kb on + strand at 6309.847 kb on - strand at 6309.847 kb on - strand at 6309.847 kb on - strand at 6309.847 kb on - strand at 6309.847 kb on - strand at 6310.050 kb on + strand, within Pf6N2E2_5491 at 6310.050 kb on + strand, within Pf6N2E2_5491 at 6310.050 kb on + strand, within Pf6N2E2_5491 at 6310.050 kb on + strand, within Pf6N2E2_5491 at 6310.050 kb on + strand, within Pf6N2E2_5491 at 6310.050 kb on + strand, within Pf6N2E2_5491 at 6310.050 kb on + strand, within Pf6N2E2_5491 at 6310.051 kb on - strand, within Pf6N2E2_5491 at 6310.051 kb on - strand, within Pf6N2E2_5491 at 6310.051 kb on - strand, within Pf6N2E2_5491 at 6310.054 kb on + strand, within Pf6N2E2_5491 at 6310.054 kb on + strand, within Pf6N2E2_5491 at 6310.055 kb on - strand, within Pf6N2E2_5491 at 6310.055 kb on - strand, within Pf6N2E2_5491 at 6310.055 kb on - strand, within Pf6N2E2_5491 at 6310.139 kb on + strand, within Pf6N2E2_5491 at 6310.140 kb on - strand, within Pf6N2E2_5491 at 6310.142 kb on + strand, within Pf6N2E2_5491 at 6310.142 kb on + strand, within Pf6N2E2_5491 at 6310.142 kb on + strand, within Pf6N2E2_5491 at 6310.143 kb on - strand, within Pf6N2E2_5491 at 6310.143 kb on - strand, within Pf6N2E2_5491 at 6310.158 kb on + strand, within Pf6N2E2_5491 at 6310.159 kb on - strand, within Pf6N2E2_5491 at 6310.249 kb on - strand, within Pf6N2E2_5491 at 6310.249 kb on - strand, within Pf6N2E2_5491 at 6310.249 kb on - strand, within Pf6N2E2_5491 at 6310.258 kb on + strand, within Pf6N2E2_5491 at 6310.258 kb on + strand, within Pf6N2E2_5491 at 6310.258 kb on + strand, within Pf6N2E2_5491 at 6310.259 kb on - strand, within Pf6N2E2_5491 at 6310.259 kb on - strand, within Pf6N2E2_5491 at 6310.259 kb on - strand, within Pf6N2E2_5491
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (C) remove 6,306,781 + Pf6N2E2_5488 0.60 -0.7 6,306,782 - Pf6N2E2_5488 0.60 -0.8 6,307,009 + Pf6N2E2_5488 0.73 +1.9 6,307,009 + Pf6N2E2_5488 0.73 -1.0 6,307,010 - Pf6N2E2_5488 0.73 +0.1 6,307,010 - Pf6N2E2_5488 0.73 +0.7 6,307,081 + Pf6N2E2_5488 0.77 +0.5 6,307,082 - Pf6N2E2_5488 0.77 +1.3 6,307,096 + Pf6N2E2_5488 0.77 -1.6 6,307,096 + Pf6N2E2_5488 0.77 +1.3 6,307,096 + Pf6N2E2_5488 0.77 +0.4 6,307,096 + Pf6N2E2_5488 0.77 +0.2 6,307,096 + Pf6N2E2_5488 0.77 -0.1 6,307,097 - Pf6N2E2_5488 0.78 -0.7 6,307,097 - Pf6N2E2_5488 0.78 +0.3 6,307,097 - Pf6N2E2_5488 0.78 +0.6 6,307,097 - Pf6N2E2_5488 0.78 -1.0 6,307,111 + Pf6N2E2_5488 0.78 +1.5 6,307,111 + Pf6N2E2_5488 0.78 +1.2 6,307,111 + Pf6N2E2_5488 0.78 -0.1 6,307,111 + Pf6N2E2_5488 0.78 -0.2 6,307,111 + Pf6N2E2_5488 0.78 +1.1 6,307,111 + Pf6N2E2_5488 0.78 -0.1 6,307,111 + Pf6N2E2_5488 0.78 -0.3 6,307,111 + Pf6N2E2_5488 0.78 +0.4 6,307,111 + Pf6N2E2_5488 0.78 -0.2 6,307,111 + Pf6N2E2_5488 0.78 +0.5 6,307,111 + Pf6N2E2_5488 0.78 -0.3 6,307,111 + Pf6N2E2_5488 0.78 +0.7 6,307,111 + Pf6N2E2_5488 0.78 -0.7 6,307,112 - Pf6N2E2_5488 0.78 -0.3 6,307,112 - Pf6N2E2_5488 0.78 -0.5 6,307,112 - Pf6N2E2_5488 0.78 +0.7 6,307,112 - Pf6N2E2_5488 0.78 -0.8 6,307,112 - Pf6N2E2_5488 0.78 -1.1 6,307,112 - Pf6N2E2_5488 0.78 +0.6 6,307,112 - Pf6N2E2_5488 0.78 +0.7 6,307,112 - Pf6N2E2_5488 0.78 +1.3 6,307,224 + Pf6N2E2_5488 0.85 +0.9 6,307,224 + Pf6N2E2_5488 0.85 -0.6 6,307,224 + Pf6N2E2_5488 0.85 -0.3 6,307,224 + Pf6N2E2_5488 0.85 +0.8 6,307,224 + Pf6N2E2_5488 0.85 +0.3 6,307,225 - Pf6N2E2_5488 0.85 -0.7 6,307,225 - Pf6N2E2_5488 0.85 -2.0 6,307,225 - Pf6N2E2_5488 0.85 -0.0 6,307,225 - Pf6N2E2_5488 0.85 -0.1 6,307,225 - Pf6N2E2_5488 0.85 -0.5 6,307,225 - Pf6N2E2_5488 0.85 +0.4 6,307,269 + Pf6N2E2_5488 0.87 +0.7 6,307,269 + Pf6N2E2_5488 0.87 +0.0 6,307,270 - Pf6N2E2_5488 0.87 -0.0 6,307,270 - Pf6N2E2_5488 0.87 +1.3 6,307,270 - Pf6N2E2_5488 0.87 -0.2 6,307,270 - Pf6N2E2_5488 0.87 +0.3 6,307,342 + -0.1 6,307,342 + +0.6 6,307,456 + +1.0 6,307,456 + +0.9 6,307,457 - -1.5 6,307,475 + +0.8 6,307,481 + +0.1 6,307,481 + +0.7 6,307,481 + +0.3 6,307,482 - -0.7 6,307,482 - -0.7 6,307,563 + -0.2 6,307,563 + +0.5 6,307,563 + +0.4 6,307,564 - +0.5 6,307,739 + +1.1 6,307,739 + +1.0 6,307,740 - -0.8 6,309,298 - +1.2 6,309,298 - +0.2 6,309,342 + Pf6N2E2_5490 0.20 -1.2 6,309,343 - Pf6N2E2_5490 0.21 +0.7 6,309,343 - Pf6N2E2_5490 0.21 +1.2 6,309,343 - Pf6N2E2_5490 0.21 -1.0 6,309,343 - Pf6N2E2_5490 0.21 -0.9 6,309,343 - Pf6N2E2_5490 0.21 +0.2 6,309,343 - Pf6N2E2_5490 0.21 +1.9 6,309,343 - Pf6N2E2_5490 0.21 -0.7 6,309,345 - Pf6N2E2_5490 0.22 -0.5 6,309,345 - Pf6N2E2_5490 0.22 +0.6 6,309,391 - Pf6N2E2_5490 0.45 +1.0 6,309,391 - Pf6N2E2_5490 0.45 -1.2 6,309,391 - Pf6N2E2_5490 0.45 -0.3 6,309,391 - Pf6N2E2_5490 0.45 +0.7 6,309,391 - Pf6N2E2_5490 0.45 +0.7 6,309,391 - Pf6N2E2_5490 0.45 +0.3 6,309,391 - Pf6N2E2_5490 0.45 -0.2 6,309,391 - Pf6N2E2_5490 0.45 -0.4 6,309,391 - Pf6N2E2_5490 0.45 -0.5 6,309,661 - +0.7 6,309,671 - +1.6 6,309,671 - +0.4 6,309,695 - -0.3 6,309,695 - +0.4 6,309,695 - -0.3 6,309,695 - +0.7 6,309,747 - +0.0 6,309,798 + +1.0 6,309,798 + -0.2 6,309,799 - +0.0 6,309,799 - +0.5 6,309,817 + -0.0 6,309,817 + +0.1 6,309,846 + +1.5 6,309,846 + -0.4 6,309,846 + +0.7 6,309,846 + +2.9 6,309,846 + +0.7 6,309,847 - +0.9 6,309,847 - +0.6 6,309,847 - +0.8 6,309,847 - +0.2 6,309,847 - -1.2 6,310,050 + Pf6N2E2_5491 0.28 -0.3 6,310,050 + Pf6N2E2_5491 0.28 +0.1 6,310,050 + Pf6N2E2_5491 0.28 +1.8 6,310,050 + Pf6N2E2_5491 0.28 -0.2 6,310,050 + Pf6N2E2_5491 0.28 +0.9 6,310,050 + Pf6N2E2_5491 0.28 +0.8 6,310,050 + Pf6N2E2_5491 0.28 -0.4 6,310,051 - Pf6N2E2_5491 0.28 +1.2 6,310,051 - Pf6N2E2_5491 0.28 -0.4 6,310,051 - Pf6N2E2_5491 0.28 -1.1 6,310,054 + Pf6N2E2_5491 0.29 +0.1 6,310,054 + Pf6N2E2_5491 0.29 -0.0 6,310,055 - Pf6N2E2_5491 0.29 +0.9 6,310,055 - Pf6N2E2_5491 0.29 +1.7 6,310,055 - Pf6N2E2_5491 0.29 +0.1 6,310,139 + Pf6N2E2_5491 0.36 +0.7 6,310,140 - Pf6N2E2_5491 0.36 +0.8 6,310,142 + Pf6N2E2_5491 0.36 +1.7 6,310,142 + Pf6N2E2_5491 0.36 +0.1 6,310,142 + Pf6N2E2_5491 0.36 +1.2 6,310,143 - Pf6N2E2_5491 0.37 -0.4 6,310,143 - Pf6N2E2_5491 0.37 +0.1 6,310,158 + Pf6N2E2_5491 0.38 +0.8 6,310,159 - Pf6N2E2_5491 0.38 -0.6 6,310,249 - Pf6N2E2_5491 0.46 +0.3 6,310,249 - Pf6N2E2_5491 0.46 -2.2 6,310,249 - Pf6N2E2_5491 0.46 +1.3 6,310,258 + Pf6N2E2_5491 0.47 -0.4 6,310,258 + Pf6N2E2_5491 0.47 +0.7 6,310,258 + Pf6N2E2_5491 0.47 +0.3 6,310,259 - Pf6N2E2_5491 0.47 -0.1 6,310,259 - Pf6N2E2_5491 0.47 -0.5 6,310,259 - Pf6N2E2_5491 0.47 +1.0
Or see this region's nucleotide sequence