Experiment: D-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5472 and Pf6N2E2_5473 are separated by 326 nucleotides Pf6N2E2_5473 and Pf6N2E2_5474 are separated by 198 nucleotides
Pf6N2E2_5472 - 5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase (EC 2.1.1.14), at 6,286,870 to 6,289,182
_5472
Pf6N2E2_5473 - hydrolase, alpha/beta fold family, at 6,289,509 to 6,290,528
_5473
Pf6N2E2_5474 - Glycine cleavage system transcriptional activator, at 6,290,727 to 6,291,608
_5474
Position (kb)
6289
6290
6291 Strain fitness (log2 ratio)
-1
0
1
2 at 6288.530 kb on - strand, within Pf6N2E2_5472 at 6288.763 kb on + strand, within Pf6N2E2_5472 at 6288.763 kb on + strand, within Pf6N2E2_5472 at 6288.763 kb on + strand, within Pf6N2E2_5472 at 6288.764 kb on - strand, within Pf6N2E2_5472 at 6288.764 kb on - strand, within Pf6N2E2_5472 at 6288.844 kb on + strand, within Pf6N2E2_5472 at 6288.845 kb on - strand, within Pf6N2E2_5472 at 6288.985 kb on + strand at 6289.049 kb on + strand at 6289.050 kb on - strand at 6289.167 kb on + strand at 6289.167 kb on + strand at 6289.275 kb on + strand at 6289.275 kb on + strand at 6289.276 kb on - strand at 6289.276 kb on - strand at 6289.276 kb on - strand at 6289.516 kb on + strand at 6289.517 kb on - strand at 6289.860 kb on + strand, within Pf6N2E2_5473 at 6289.860 kb on + strand, within Pf6N2E2_5473 at 6289.860 kb on + strand, within Pf6N2E2_5473 at 6289.861 kb on - strand, within Pf6N2E2_5473 at 6289.861 kb on - strand, within Pf6N2E2_5473 at 6289.862 kb on + strand, within Pf6N2E2_5473 at 6289.862 kb on + strand, within Pf6N2E2_5473 at 6289.862 kb on + strand, within Pf6N2E2_5473 at 6289.862 kb on + strand, within Pf6N2E2_5473 at 6289.862 kb on + strand, within Pf6N2E2_5473 at 6289.862 kb on + strand, within Pf6N2E2_5473 at 6289.862 kb on + strand, within Pf6N2E2_5473 at 6289.862 kb on + strand, within Pf6N2E2_5473 at 6289.862 kb on + strand, within Pf6N2E2_5473 at 6289.863 kb on - strand, within Pf6N2E2_5473 at 6289.863 kb on - strand, within Pf6N2E2_5473 at 6289.863 kb on - strand, within Pf6N2E2_5473 at 6289.863 kb on - strand, within Pf6N2E2_5473 at 6289.863 kb on - strand, within Pf6N2E2_5473 at 6289.863 kb on - strand, within Pf6N2E2_5473 at 6289.863 kb on - strand, within Pf6N2E2_5473 at 6289.930 kb on + strand, within Pf6N2E2_5473 at 6289.930 kb on + strand, within Pf6N2E2_5473 at 6289.930 kb on + strand, within Pf6N2E2_5473 at 6289.931 kb on - strand, within Pf6N2E2_5473 at 6290.196 kb on + strand, within Pf6N2E2_5473 at 6290.197 kb on - strand, within Pf6N2E2_5473 at 6290.197 kb on - strand, within Pf6N2E2_5473 at 6290.197 kb on - strand, within Pf6N2E2_5473 at 6290.224 kb on + strand, within Pf6N2E2_5473 at 6290.225 kb on - strand, within Pf6N2E2_5473 at 6290.225 kb on - strand, within Pf6N2E2_5473 at 6290.454 kb on + strand at 6290.455 kb on - strand at 6290.614 kb on + strand at 6291.144 kb on + strand, within Pf6N2E2_5474 at 6291.144 kb on + strand, within Pf6N2E2_5474 at 6291.144 kb on + strand, within Pf6N2E2_5474 at 6291.144 kb on + strand, within Pf6N2E2_5474 at 6291.144 kb on + strand, within Pf6N2E2_5474 at 6291.144 kb on + strand, within Pf6N2E2_5474 at 6291.144 kb on + strand, within Pf6N2E2_5474 at 6291.144 kb on + strand, within Pf6N2E2_5474 at 6291.144 kb on + strand, within Pf6N2E2_5474 at 6291.144 kb on + strand, within Pf6N2E2_5474 at 6291.144 kb on + strand, within Pf6N2E2_5474 at 6291.144 kb on + strand, within Pf6N2E2_5474 at 6291.145 kb on - strand, within Pf6N2E2_5474 at 6291.145 kb on - strand, within Pf6N2E2_5474 at 6291.145 kb on - strand, within Pf6N2E2_5474 at 6291.145 kb on - strand, within Pf6N2E2_5474 at 6291.145 kb on - strand, within Pf6N2E2_5474 at 6291.145 kb on - strand, within Pf6N2E2_5474 at 6291.145 kb on - strand, within Pf6N2E2_5474 at 6291.145 kb on - strand, within Pf6N2E2_5474 at 6291.145 kb on - strand, within Pf6N2E2_5474
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (C) remove 6,288,530 - Pf6N2E2_5472 0.72 -1.4 6,288,763 + Pf6N2E2_5472 0.82 -0.0 6,288,763 + Pf6N2E2_5472 0.82 -0.5 6,288,763 + Pf6N2E2_5472 0.82 +0.8 6,288,764 - Pf6N2E2_5472 0.82 +1.4 6,288,764 - Pf6N2E2_5472 0.82 +0.5 6,288,844 + Pf6N2E2_5472 0.85 -1.0 6,288,845 - Pf6N2E2_5472 0.85 +0.3 6,288,985 + +0.2 6,289,049 + -0.3 6,289,050 - -0.1 6,289,167 + -0.3 6,289,167 + +0.7 6,289,275 + -0.1 6,289,275 + +0.8 6,289,276 - +1.4 6,289,276 - +1.4 6,289,276 - -1.0 6,289,516 + -0.5 6,289,517 - -0.2 6,289,860 + Pf6N2E2_5473 0.34 +0.3 6,289,860 + Pf6N2E2_5473 0.34 -0.9 6,289,860 + Pf6N2E2_5473 0.34 +1.3 6,289,861 - Pf6N2E2_5473 0.35 -0.0 6,289,861 - Pf6N2E2_5473 0.35 +0.3 6,289,862 + Pf6N2E2_5473 0.35 +1.6 6,289,862 + Pf6N2E2_5473 0.35 +0.4 6,289,862 + Pf6N2E2_5473 0.35 +0.2 6,289,862 + Pf6N2E2_5473 0.35 +1.1 6,289,862 + Pf6N2E2_5473 0.35 +1.0 6,289,862 + Pf6N2E2_5473 0.35 +0.7 6,289,862 + Pf6N2E2_5473 0.35 -0.0 6,289,862 + Pf6N2E2_5473 0.35 +0.1 6,289,862 + Pf6N2E2_5473 0.35 +0.3 6,289,863 - Pf6N2E2_5473 0.35 +0.2 6,289,863 - Pf6N2E2_5473 0.35 -0.4 6,289,863 - Pf6N2E2_5473 0.35 +1.3 6,289,863 - Pf6N2E2_5473 0.35 +0.1 6,289,863 - Pf6N2E2_5473 0.35 +0.8 6,289,863 - Pf6N2E2_5473 0.35 -1.0 6,289,863 - Pf6N2E2_5473 0.35 -0.5 6,289,930 + Pf6N2E2_5473 0.41 +0.1 6,289,930 + Pf6N2E2_5473 0.41 +1.5 6,289,930 + Pf6N2E2_5473 0.41 -0.6 6,289,931 - Pf6N2E2_5473 0.41 -0.1 6,290,196 + Pf6N2E2_5473 0.67 +0.7 6,290,197 - Pf6N2E2_5473 0.67 +1.0 6,290,197 - Pf6N2E2_5473 0.67 +1.0 6,290,197 - Pf6N2E2_5473 0.67 -1.2 6,290,224 + Pf6N2E2_5473 0.70 +0.0 6,290,225 - Pf6N2E2_5473 0.70 +2.5 6,290,225 - Pf6N2E2_5473 0.70 +0.1 6,290,454 + -0.2 6,290,455 - -1.1 6,290,614 + -0.2 6,291,144 + Pf6N2E2_5474 0.47 +0.6 6,291,144 + Pf6N2E2_5474 0.47 -0.9 6,291,144 + Pf6N2E2_5474 0.47 +0.1 6,291,144 + Pf6N2E2_5474 0.47 -0.1 6,291,144 + Pf6N2E2_5474 0.47 +0.8 6,291,144 + Pf6N2E2_5474 0.47 -0.4 6,291,144 + Pf6N2E2_5474 0.47 -0.9 6,291,144 + Pf6N2E2_5474 0.47 +0.3 6,291,144 + Pf6N2E2_5474 0.47 +2.0 6,291,144 + Pf6N2E2_5474 0.47 +0.4 6,291,144 + Pf6N2E2_5474 0.47 +0.2 6,291,144 + Pf6N2E2_5474 0.47 -0.1 6,291,145 - Pf6N2E2_5474 0.47 +0.8 6,291,145 - Pf6N2E2_5474 0.47 +0.9 6,291,145 - Pf6N2E2_5474 0.47 +1.0 6,291,145 - Pf6N2E2_5474 0.47 +1.0 6,291,145 - Pf6N2E2_5474 0.47 -0.7 6,291,145 - Pf6N2E2_5474 0.47 +0.6 6,291,145 - Pf6N2E2_5474 0.47 +0.2 6,291,145 - Pf6N2E2_5474 0.47 +0.7 6,291,145 - Pf6N2E2_5474 0.47 -1.1
Or see this region's nucleotide sequence